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β-Casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial-mesenchymal transition of renal tubular epithelial cells.
Peptides. 2012 Aug; 36(2):186-91.P

Abstract

This study was designed to investigate the putative protective effect of β-casomorphin-7 on diabetic nephropathy in a rat model, and to explore the possible mechanism of this effect. SD rats were randomly divided into the following three groups: control group, diabetes group and β-casomorphin-7-treatment group. All rats were euthanized after 30 days with or without β-casomorphin-7 treatment. Biochemical parameters including blood glucose and renal function were quantified. The concentration of plasma TGF-β1 was measured by ELISA. Histopathological changes to the kidney were studied by Masson and Sirius red staining. Expressions of α-smooth muscle actin (α-SMA), E-cadherin, vimentin, cytokeratin19 and TGF-β1 mRNA in rat renal cortices were analyzed by real-time PCR. Changes in α-SMA and E-cadherin protein expression in rat renal cortices were quantified by Western blot. β-Casomorphin-7 treatment of diabetic rats reduced urinary glucose, urinary protein, serum creatinine, blood urinary nitrogen, plasma TGF-β1 and the ratio of kidney: body weight. Masson and Sirius red staining showed that β-casomorphin-7 treatment attenuated renal interstitial fibrosis in diabetic rats. Compared to the control rats, diabetic rats had elevated expressions of α-SMA, vimentin and TGF-β1 mRNA and α -SMA protein and decreased expression of E-cadherin and cytokeratin19 mRNA, and E-cadherin protein. β-Casomorphin-7 treatment of diabetic rats partially normalized these changes. Our results suggest that administration of β-casomorphin-7 attenuates renal interstitial fibrosis caused by diabetes. This protective effect may be associated, in part, with down regulation of epithelial-mesenchymal transition of renal tubular epithelial cells.

Authors+Show Affiliations

Nanjing Agriculture University, Nanjing, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22687367

Citation

Zhang, Wei, et al. "Β-Casomorphin-7 Attenuates the Development of Nephropathy in Type I Diabetes Via Inhibition of Epithelial-mesenchymal Transition of Renal Tubular Epithelial Cells." Peptides, vol. 36, no. 2, 2012, pp. 186-91.
Zhang W, Miao J, Ma C, et al. Β-Casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial-mesenchymal transition of renal tubular epithelial cells. Peptides. 2012;36(2):186-91.
Zhang, W., Miao, J., Ma, C., Han, D., & Zhang, Y. (2012). Β-Casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial-mesenchymal transition of renal tubular epithelial cells. Peptides, 36(2), 186-91. https://doi.org/10.1016/j.peptides.2012.05.022
Zhang W, et al. Β-Casomorphin-7 Attenuates the Development of Nephropathy in Type I Diabetes Via Inhibition of Epithelial-mesenchymal Transition of Renal Tubular Epithelial Cells. Peptides. 2012;36(2):186-91. PubMed PMID: 22687367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - β-Casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial-mesenchymal transition of renal tubular epithelial cells. AU - Zhang,Wei, AU - Miao,Jinfeng, AU - Ma,Chang, AU - Han,Dongning, AU - Zhang,Yuanshu, Y1 - 2012/06/09/ PY - 2012/04/04/received PY - 2012/05/31/revised PY - 2012/05/31/accepted PY - 2012/6/13/entrez PY - 2012/6/13/pubmed PY - 2012/12/29/medline SP - 186 EP - 91 JF - Peptides JO - Peptides VL - 36 IS - 2 N2 - This study was designed to investigate the putative protective effect of β-casomorphin-7 on diabetic nephropathy in a rat model, and to explore the possible mechanism of this effect. SD rats were randomly divided into the following three groups: control group, diabetes group and β-casomorphin-7-treatment group. All rats were euthanized after 30 days with or without β-casomorphin-7 treatment. Biochemical parameters including blood glucose and renal function were quantified. The concentration of plasma TGF-β1 was measured by ELISA. Histopathological changes to the kidney were studied by Masson and Sirius red staining. Expressions of α-smooth muscle actin (α-SMA), E-cadherin, vimentin, cytokeratin19 and TGF-β1 mRNA in rat renal cortices were analyzed by real-time PCR. Changes in α-SMA and E-cadherin protein expression in rat renal cortices were quantified by Western blot. β-Casomorphin-7 treatment of diabetic rats reduced urinary glucose, urinary protein, serum creatinine, blood urinary nitrogen, plasma TGF-β1 and the ratio of kidney: body weight. Masson and Sirius red staining showed that β-casomorphin-7 treatment attenuated renal interstitial fibrosis in diabetic rats. Compared to the control rats, diabetic rats had elevated expressions of α-SMA, vimentin and TGF-β1 mRNA and α -SMA protein and decreased expression of E-cadherin and cytokeratin19 mRNA, and E-cadherin protein. β-Casomorphin-7 treatment of diabetic rats partially normalized these changes. Our results suggest that administration of β-casomorphin-7 attenuates renal interstitial fibrosis caused by diabetes. This protective effect may be associated, in part, with down regulation of epithelial-mesenchymal transition of renal tubular epithelial cells. SN - 1873-5169 UR - https://www.unboundmedicine.com/medline/citation/22687367/β_Casomorphin_7_attenuates_the_development_of_nephropathy_in_type_I_diabetes_via_inhibition_of_epithelial_mesenchymal_transition_of_renal_tubular_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(12)00256-2 DB - PRIME DP - Unbound Medicine ER -