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Sub-toxic effects of CuO nanoparticles on bacteria: kinetics, role of Cu ions and possible mechanisms of action.
Environ Pollut. 2012 Oct; 169:81-9.EP

Abstract

The sub-toxic effects of CuO nanoparticles (nano-CuO) were evaluated using three recombinant luminescent Escherichia coli bacteria responding specifically to (i) reactive oxygen species (ROS), (ii) single-stranded DNA breaks and (iii) bioavailable Cu ions. Using these sensors we showed that nano-CuO induces the formation of superoxide anions, hydrogen peroxide and single-stranded DNA already at very low sub-toxic levels (0.1 mg Cu/L). The maximal sub-toxic response of all biosensors to nominal concentrations of nano-CuO, micro-CuO (size control) and CuSO(4) (solubility control) occurred at ~6, ~600 and ~0.6 mg Cu/L, respectively. According to the chemical analysis all the latter concentrations yielded ~0.6 mg of soluble Cu/L, indicating that dissolution of CuO particles was the key factor triggering the ROS and DNA damage responses in bacteria. Cu-ions chelation studies also showed that CuO particles were not involved in these stress responses. The solubilization results were confirmed by Pseudomonas fluorescens Cu-ion sensor.

Authors+Show Affiliations

Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia. olesja.bondarenko@kbfi.eeNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22694973

Citation

Bondarenko, Olesja, et al. "Sub-toxic Effects of CuO Nanoparticles On Bacteria: Kinetics, Role of Cu Ions and Possible Mechanisms of Action." Environmental Pollution (Barking, Essex : 1987), vol. 169, 2012, pp. 81-9.
Bondarenko O, Ivask A, Käkinen A, et al. Sub-toxic effects of CuO nanoparticles on bacteria: kinetics, role of Cu ions and possible mechanisms of action. Environ Pollut. 2012;169:81-9.
Bondarenko, O., Ivask, A., Käkinen, A., & Kahru, A. (2012). Sub-toxic effects of CuO nanoparticles on bacteria: kinetics, role of Cu ions and possible mechanisms of action. Environmental Pollution (Barking, Essex : 1987), 169, 81-9. https://doi.org/10.1016/j.envpol.2012.05.009
Bondarenko O, et al. Sub-toxic Effects of CuO Nanoparticles On Bacteria: Kinetics, Role of Cu Ions and Possible Mechanisms of Action. Environ Pollut. 2012;169:81-9. PubMed PMID: 22694973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sub-toxic effects of CuO nanoparticles on bacteria: kinetics, role of Cu ions and possible mechanisms of action. AU - Bondarenko,Olesja, AU - Ivask,Angela, AU - Käkinen,Aleksandr, AU - Kahru,Anne, Y1 - 2012/06/12/ PY - 2012/03/21/received PY - 2012/04/25/revised PY - 2012/05/08/accepted PY - 2012/6/15/entrez PY - 2012/6/15/pubmed PY - 2012/11/14/medline SP - 81 EP - 9 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 169 N2 - The sub-toxic effects of CuO nanoparticles (nano-CuO) were evaluated using three recombinant luminescent Escherichia coli bacteria responding specifically to (i) reactive oxygen species (ROS), (ii) single-stranded DNA breaks and (iii) bioavailable Cu ions. Using these sensors we showed that nano-CuO induces the formation of superoxide anions, hydrogen peroxide and single-stranded DNA already at very low sub-toxic levels (0.1 mg Cu/L). The maximal sub-toxic response of all biosensors to nominal concentrations of nano-CuO, micro-CuO (size control) and CuSO(4) (solubility control) occurred at ~6, ~600 and ~0.6 mg Cu/L, respectively. According to the chemical analysis all the latter concentrations yielded ~0.6 mg of soluble Cu/L, indicating that dissolution of CuO particles was the key factor triggering the ROS and DNA damage responses in bacteria. Cu-ions chelation studies also showed that CuO particles were not involved in these stress responses. The solubilization results were confirmed by Pseudomonas fluorescens Cu-ion sensor. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/22694973/Sub_toxic_effects_of_CuO_nanoparticles_on_bacteria:_kinetics_role_of_Cu_ions_and_possible_mechanisms_of_action_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(12)00248-5 DB - PRIME DP - Unbound Medicine ER -