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Characterization of the n-3 polyunsaturated fatty acid enrichment in laying hens fed an extruded flax enrichment source.
Poult Sci. 2012 Jul; 91(7):1720-32.PS

Abstract

The time required to reach a plateau of n-3 polyunsaturated fatty acid (PUFA) concentration in plasma and egg yolk and dynamics of the enrichment process were examined in laying hens. A group of 75 Lohmann White Leghorn layers (65 wk) were fed one of 3 diets: control, moderate, or high n-3 PUFA-enriched diet for 18 d. Diets provided similar ME and CP and contained 0, 7.5%, or 15% LinPRO (source of n-3 PUFA), respectively. Prior to dietary treatment, baseline values were established for the BW, fatty acid composition in egg yolk on a whole-egg basis, and in plasma. These measurements were repeated at 6, 12, and 18 d of feeding. Enzymatic conversion rates of linolenic acid (LNA) to long-chain fatty acids were calculated. Data were analyzed with Proc Mixed of SAS, and broken stick analysis was used to determine n-3 PUFA plateau using the NLIN procedure of SAS (P < 0.05). The total egg yolk n-3 PUFA reached a plateau of 343.7 mg/egg and 272.9 mg/egg after 6.6 and 5.9 d on the high and moderate diets, respectively. In blood plasma, the n-3 PUFA concentrations reached saturation in 7.2 d with 0.93 mg/mL and 0.67 mg/mL on high and moderate diets, respectively. The transfer efficiency of total n-3 PUFA from the diet to the egg yolk was calculated as 55.6% in control birds, 30.5% in moderate birds, and 22.2% in high birds, demonstrating reduced transfer efficiency of n-3 PUFA as inclusion in the feed increases. Final egg yolk n-3 PUFA concentrations had a CV of 16.5% compared with 28.5% for plasma. After 12 d, the long-chain n-3 PUFA [eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA)] were significantly higher in egg yolk from hens on the moderate and high enriched diets compared with those from hens fed the control diet, whereas in plasma values did not differ. Broken stick analysis of egg enrichment indicated that high birds reached the target threshold of 300 mg of total n-3 PUFA/egg in 5 d. A significant increase in EPA, DPA, and DHA and reduction in arachidonic acid content in egg yolks from hens fed enriched diets compared with the control diet confirms competition for enzymes during postabsorptive modification of these fatty acids. This work contributes to the understanding of individual hen effects on n-3 PUFA absorption and the effect of level of dietary enrichment with an extruded flax product on final yolk n-3 PUFA concentration.

Authors+Show Affiliations

Department of Agriculture, University of Alberta, Edmonton, AB, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22700520

Citation

Nain, S, et al. "Characterization of the N-3 Polyunsaturated Fatty Acid Enrichment in Laying Hens Fed an Extruded Flax Enrichment Source." Poultry Science, vol. 91, no. 7, 2012, pp. 1720-32.
Nain S, Renema RA, Korver DR, et al. Characterization of the n-3 polyunsaturated fatty acid enrichment in laying hens fed an extruded flax enrichment source. Poult Sci. 2012;91(7):1720-32.
Nain, S., Renema, R. A., Korver, D. R., & Zuidhof, M. J. (2012). Characterization of the n-3 polyunsaturated fatty acid enrichment in laying hens fed an extruded flax enrichment source. Poultry Science, 91(7), 1720-32. https://doi.org/10.3382/ps.2011-02048
Nain S, et al. Characterization of the N-3 Polyunsaturated Fatty Acid Enrichment in Laying Hens Fed an Extruded Flax Enrichment Source. Poult Sci. 2012;91(7):1720-32. PubMed PMID: 22700520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the n-3 polyunsaturated fatty acid enrichment in laying hens fed an extruded flax enrichment source. AU - Nain,S, AU - Renema,R A, AU - Korver,D R, AU - Zuidhof,M J, PY - 2012/6/16/entrez PY - 2012/6/16/pubmed PY - 2012/8/24/medline SP - 1720 EP - 32 JF - Poultry science JO - Poult. Sci. VL - 91 IS - 7 N2 - The time required to reach a plateau of n-3 polyunsaturated fatty acid (PUFA) concentration in plasma and egg yolk and dynamics of the enrichment process were examined in laying hens. A group of 75 Lohmann White Leghorn layers (65 wk) were fed one of 3 diets: control, moderate, or high n-3 PUFA-enriched diet for 18 d. Diets provided similar ME and CP and contained 0, 7.5%, or 15% LinPRO (source of n-3 PUFA), respectively. Prior to dietary treatment, baseline values were established for the BW, fatty acid composition in egg yolk on a whole-egg basis, and in plasma. These measurements were repeated at 6, 12, and 18 d of feeding. Enzymatic conversion rates of linolenic acid (LNA) to long-chain fatty acids were calculated. Data were analyzed with Proc Mixed of SAS, and broken stick analysis was used to determine n-3 PUFA plateau using the NLIN procedure of SAS (P < 0.05). The total egg yolk n-3 PUFA reached a plateau of 343.7 mg/egg and 272.9 mg/egg after 6.6 and 5.9 d on the high and moderate diets, respectively. In blood plasma, the n-3 PUFA concentrations reached saturation in 7.2 d with 0.93 mg/mL and 0.67 mg/mL on high and moderate diets, respectively. The transfer efficiency of total n-3 PUFA from the diet to the egg yolk was calculated as 55.6% in control birds, 30.5% in moderate birds, and 22.2% in high birds, demonstrating reduced transfer efficiency of n-3 PUFA as inclusion in the feed increases. Final egg yolk n-3 PUFA concentrations had a CV of 16.5% compared with 28.5% for plasma. After 12 d, the long-chain n-3 PUFA [eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA)] were significantly higher in egg yolk from hens on the moderate and high enriched diets compared with those from hens fed the control diet, whereas in plasma values did not differ. Broken stick analysis of egg enrichment indicated that high birds reached the target threshold of 300 mg of total n-3 PUFA/egg in 5 d. A significant increase in EPA, DPA, and DHA and reduction in arachidonic acid content in egg yolks from hens fed enriched diets compared with the control diet confirms competition for enzymes during postabsorptive modification of these fatty acids. This work contributes to the understanding of individual hen effects on n-3 PUFA absorption and the effect of level of dietary enrichment with an extruded flax product on final yolk n-3 PUFA concentration. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/22700520/Characterization_of_the_n_3_polyunsaturated_fatty_acid_enrichment_in_laying_hens_fed_an_extruded_flax_enrichment_source_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)40302-7 DB - PRIME DP - Unbound Medicine ER -