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Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil.
J Hazard Mater. 2013 Nov 15; 262:1245-58.JH

Abstract

Many studies demonstrate the potential application of arbuscular mycorrhizal fungi (AMF) for remediation purposes, but little is known on AMF potential to enhance plant tolerance to arsenic (As) and the mechanisms involved in this process. We carried anatomical and ultrastructural studies to examine this symbiotic association and the characteristics of shoots and roots of Leucaena leucocephala in As-amended soils (35 and 75 mg As dm(-3)). The experiment used 3 AMF isolates from uncontaminated soils: Acaulospora morrowiae, Glomus clarum, and Gigaspora albida; a mixed inoculum derived from combining these 3 isolates (named Mix AMF); and, 3 AMF isolates from As-contaminated areas: A. morrowiae, G. clarum and Paraglomus occultum. Phytotoxicity symptoms due to arsenic contamination appeared during plant growth, especially in treatments without AMF application. Inoculation with G. clarum and the mixture of species (A. morrowiae, G. albida, and G. clarum) resulted in better growth of L. leucocephala in soils with high As concentrations, as well as significant As removal from the soil, showing a potential for using AMF in phytoextraction. Light microscopy (LS), transmission (TEM) and scanning electron microscopies (SEM) studies showed the colonization of the AMF in plant tissues and damage in all treatments, with ultrastructural changes being observed in leaves and roots of L. leucocephala, especially with the addition of 75 mg dm(-3) of As.

Authors+Show Affiliations

Departamento de Ciência do Solo, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, Minas Gerais, 37200-000, Brazil. Electronic address: jerusaschneider@hotmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22704769

Citation

Schneider, Jerusa, et al. "Anatomy and Ultrastructure Alterations of Leucaena Leucocephala (Lam.) Inoculated With Mycorrhizal Fungi in Response to Arsenic-contaminated Soil." Journal of Hazardous Materials, vol. 262, 2013, pp. 1245-58.
Schneider J, Labory CR, Rangel WM, et al. Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil. J Hazard Mater. 2013;262:1245-58.
Schneider, J., Labory, C. R., Rangel, W. M., Alves, E., & Guilherme, L. R. (2013). Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil. Journal of Hazardous Materials, 262, 1245-58. https://doi.org/10.1016/j.jhazmat.2012.05.091
Schneider J, et al. Anatomy and Ultrastructure Alterations of Leucaena Leucocephala (Lam.) Inoculated With Mycorrhizal Fungi in Response to Arsenic-contaminated Soil. J Hazard Mater. 2013 Nov 15;262:1245-58. PubMed PMID: 22704769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil. AU - Schneider,Jerusa, AU - Labory,Claudia Regina Gontijo, AU - Rangel,Wesley Melo, AU - Alves,Eduardo, AU - Guilherme,Luiz Roberto Guimarães, Y1 - 2012/06/05/ PY - 2011/12/07/received PY - 2012/05/05/revised PY - 2012/05/25/accepted PY - 2012/6/19/entrez PY - 2012/6/19/pubmed PY - 2014/7/16/medline KW - Electron microscopy KW - Light microscopy KW - Mycorrhizae KW - Phytoprotection SP - 1245 EP - 58 JF - Journal of hazardous materials JO - J Hazard Mater VL - 262 N2 - Many studies demonstrate the potential application of arbuscular mycorrhizal fungi (AMF) for remediation purposes, but little is known on AMF potential to enhance plant tolerance to arsenic (As) and the mechanisms involved in this process. We carried anatomical and ultrastructural studies to examine this symbiotic association and the characteristics of shoots and roots of Leucaena leucocephala in As-amended soils (35 and 75 mg As dm(-3)). The experiment used 3 AMF isolates from uncontaminated soils: Acaulospora morrowiae, Glomus clarum, and Gigaspora albida; a mixed inoculum derived from combining these 3 isolates (named Mix AMF); and, 3 AMF isolates from As-contaminated areas: A. morrowiae, G. clarum and Paraglomus occultum. Phytotoxicity symptoms due to arsenic contamination appeared during plant growth, especially in treatments without AMF application. Inoculation with G. clarum and the mixture of species (A. morrowiae, G. albida, and G. clarum) resulted in better growth of L. leucocephala in soils with high As concentrations, as well as significant As removal from the soil, showing a potential for using AMF in phytoextraction. Light microscopy (LS), transmission (TEM) and scanning electron microscopies (SEM) studies showed the colonization of the AMF in plant tissues and damage in all treatments, with ultrastructural changes being observed in leaves and roots of L. leucocephala, especially with the addition of 75 mg dm(-3) of As. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/22704769/Anatomy_and_ultrastructure_alterations_of_Leucaena_leucocephala__Lam___inoculated_with_mycorrhizal_fungi_in_response_to_arsenic_contaminated_soil_ DB - PRIME DP - Unbound Medicine ER -