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Evaluation of antitumor activity and in vivo antioxidant status of Anthocephalus cadamba on Ehrlich ascites carcinoma treated mice.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Anthocephalus cadamba (Roxb.) Miq. (Family: Rubiaceae) is commonly known as "Kadamba" in Sanskrit and Hindi in India. Various parts of this plant have been used as a folk medicine for the treatment of tumor, wound healing, inflammation and as a hypoglycemic agent.

AIM OF STUDY

The purpose of this investigation was to evaluate the antitumor activity and antioxidant status of defatted methanol extract of A. cadamba (MEAC) on Ehrlich ascites carcinoma (EAC) treated mice.

MATERIALS AND METHODS

In vitro cytotoxicity assay has been evaluated by using the trypan blue method. The determination of in vivo antitumor activity was performed by using different EAC cells (2 × 10(6) cells, i.p.) inoculated mice groups (n=12). The groups were treated for 9 consecutive days with MEAC at the doses of 200 and 400 mg/kg b.w. respectively. After 24h of last dose and 18 h of fasting, half of the mice were sacrificed and the rest were kept alive for assessment of increase in life span. The antitumor potential of MEAC was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating liver and kidney tissue enzymes.

RESULTS

MEAC showed direct cytotoxicity on EAC cell line in a dose dependant manner. MEAC exhibited significant (P<0.01) decrease in the tumor volume, viable cell count, tumor weight and elevated the life span of EAC tumor bearing mice. The hematological profile, biochemical estimations and tissue antioxidant assay were reverted to normal level in MEAC treated mice.

CONCLUSION

Experimental results revealed that MEAC possesses potent antitumor and antioxidant properties. Further research is going on to find out the active principle(s) of MEAC for better understanding of mechanism of its antitumor and antioxidant activity.

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  • Authors+Show Affiliations

    ,

    Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, WB, India.

    , , , ,

    Source

    Journal of ethnopharmacology 142:3 2012 Aug 1 pg 865-70

    MeSH

    Animals
    Antineoplastic Agents, Phytogenic
    Antioxidants
    Carcinoma, Ehrlich Tumor
    Catalase
    Cell Line, Tumor
    Glutathione
    Mice
    Phytotherapy
    Plant Bark
    Plant Extracts
    Rubiaceae
    Superoxide Dismutase
    Tumor Burden

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22705911