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Neuroprotective effect of saponin rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain.
J Ethnopharmacol. 2012 Aug 01; 142(3):723-31.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Traditionally, Acorus calamus has been used for the treatment and management of headache, migraine, body ache and severe inflammatory pain in the Unani, Ayurveda and Indian system of medicine.

AIM OF THE STUDY

Present study focuses on the evaluation of saponin rich extract of Acorus calamus (SRE-AC) in chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain and neuronal functional changes in rats.

MATERIALS AND METHODS

The pain sensitive tests, i.e., thermal and mechanical hyperalgesia and sciatic functional index test, were performed on the different days, i.e., days 0, 1, 7, 14, and 21. The motor and sensory nerve conduction velocity was also measured on the 21st day. Tissue total protein, superoxide anion generation, total calcium, myeloperoxidase and TNF-α levels were estimated to assess biochemical changes. Histopathological evaluations were also performed. SRE-AC (20 and 40 mg/kg) and pregabalin (10mg/kg, serving as a positive control) were administered orally for 14 consecutive days from the day of surgery.

RESULTS

CCI produced significant (P<0.05) increase in thermal and mechanical hyperalgesia, rise in sciatic functional index, decrease in nerve conduction velocity, along with biochemical and histopathological changes. Oral administration of SRE-AC and pregabalin significantly (P<0.05) ameliorated CCI-induced nociceptive pain threshold, sciatic functional and electrophysiological changes in a dose dependent manner. Further, tissue biochemical and histopathological changes were also attenuated.

CONCLUSION

SRE-AC has shown ameliorative effect in CCI-induced neuropathic pain which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory and neuroprotective actions.

Authors+Show Affiliations

Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22706151

Citation

Muthuraman, Arunachalam, and Nirmal Singh. "Neuroprotective Effect of Saponin Rich Extract of Acorus Calamus L. in Rat Model of Chronic Constriction Injury (CCI) of Sciatic Nerve-induced Neuropathic Pain." Journal of Ethnopharmacology, vol. 142, no. 3, 2012, pp. 723-31.
Muthuraman A, Singh N. Neuroprotective effect of saponin rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain. J Ethnopharmacol. 2012;142(3):723-31.
Muthuraman, A., & Singh, N. (2012). Neuroprotective effect of saponin rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain. Journal of Ethnopharmacology, 142(3), 723-31. https://doi.org/10.1016/j.jep.2012.05.049
Muthuraman A, Singh N. Neuroprotective Effect of Saponin Rich Extract of Acorus Calamus L. in Rat Model of Chronic Constriction Injury (CCI) of Sciatic Nerve-induced Neuropathic Pain. J Ethnopharmacol. 2012 Aug 1;142(3):723-31. PubMed PMID: 22706151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of saponin rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain. AU - Muthuraman,Arunachalam, AU - Singh,Nirmal, Y1 - 2012/06/15/ PY - 2012/01/18/received PY - 2012/05/06/revised PY - 2012/05/25/accepted PY - 2012/6/19/entrez PY - 2012/6/19/pubmed PY - 2012/12/15/medline SP - 723 EP - 31 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Traditionally, Acorus calamus has been used for the treatment and management of headache, migraine, body ache and severe inflammatory pain in the Unani, Ayurveda and Indian system of medicine. AIM OF THE STUDY: Present study focuses on the evaluation of saponin rich extract of Acorus calamus (SRE-AC) in chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain and neuronal functional changes in rats. MATERIALS AND METHODS: The pain sensitive tests, i.e., thermal and mechanical hyperalgesia and sciatic functional index test, were performed on the different days, i.e., days 0, 1, 7, 14, and 21. The motor and sensory nerve conduction velocity was also measured on the 21st day. Tissue total protein, superoxide anion generation, total calcium, myeloperoxidase and TNF-α levels were estimated to assess biochemical changes. Histopathological evaluations were also performed. SRE-AC (20 and 40 mg/kg) and pregabalin (10mg/kg, serving as a positive control) were administered orally for 14 consecutive days from the day of surgery. RESULTS: CCI produced significant (P<0.05) increase in thermal and mechanical hyperalgesia, rise in sciatic functional index, decrease in nerve conduction velocity, along with biochemical and histopathological changes. Oral administration of SRE-AC and pregabalin significantly (P<0.05) ameliorated CCI-induced nociceptive pain threshold, sciatic functional and electrophysiological changes in a dose dependent manner. Further, tissue biochemical and histopathological changes were also attenuated. CONCLUSION: SRE-AC has shown ameliorative effect in CCI-induced neuropathic pain which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory and neuroprotective actions. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22706151/Neuroprotective_effect_of_saponin_rich_extract_of_Acorus_calamus_L__in_rat_model_of_chronic_constriction_injury__CCI__of_sciatic_nerve_induced_neuropathic_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00371-6 DB - PRIME DP - Unbound Medicine ER -