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Quantitative NMR: an applicable method for quantitative analysis of medicinal plant extracts and herbal products.
Phytochem Anal. 2012 Nov-Dec; 23(6):689-96.PA

Abstract

INTRODUCTION

Quantitative analysis and standardisation of plant extracts or herbal products is a tedious process requiring time-consuming sample preparation and analytical method development for the resolution of analyte peaks from the complex natural extract. Quantitative analysis by HPLC requires a pure authentic standard of the compound being quantified. We report here a quantitative NMR (qNMR) method for quantitative analysis of three medicinal plant extracts and their herbal products without the need of authentic standards. Quantitation can be done by using any commercially available pure sample as an internal reference standard.

OBJECTIVE

To develop a reliable method for standardisation and quantitative analysis of extracts from medicinal plants Eugenia jambolana, Withania somnifera and Aegle marmelos and their herbal products using qNMR.

METHODOLOGY

The (1) H-NMR spectra of known amounts of crude plant extracts with internal standards were recorded in deuterated solvents and quantitation was performed by calculating the relative ratio of the peak area of selected proton signals of the target compounds and the internal reference standard. Anthocyanins [delphinidin-3,5-diglucoside (1), petunidin-3,5-diglucoside (2) and malvidin-3,5-diglucoside (3)] for E. jambolana fruit extract and imperatorin (4) for A. marmelos fruit extract were selected as marker constituents for quantitation and 1,3,5-trimethoxybenzene (TMB) was used as an internal reference standard. Total withanolide content was determined for W. somnifera using 2,4-diformyl phloroglucinol as an internal reference standard.

RESULTS

The (1) H-NMR gave a linear response for the marker constituents, anthocyanins, withaferin A and imperatorin. Using the described method, the amount of anthocyanins in Amberlite(R) XAD7HP and Sephadex enriched extracts of E. jambolana was 3.77% and 9.57% (delphinidin-3,5-diglucoside), 4.72% and 12.0% (petunidin-3,5-diglucoside), 6.55% and 15.70% (malvidin-3,5-diglucoside), respectively. The imperatorin content was 0.424% in A. marmelos fruit and 0.090 % and 0.114% in sharbat and candies. Total withanolides content was 0.191% in the chloroform extract and 0.234% in the capsule extract. These values are in accordance with HPLC results.

CONCLUSION

This qNMR technique could be used for NMR fingerprinting and quantitation for the purpose of quality control and standardisation of many plant-based herbal products and medicines and has certain advantages over HPLC.

Authors+Show Affiliations

Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Punjab -, 160062, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22707000

Citation

Chauthe, Siddheshwar K., et al. "Quantitative NMR: an Applicable Method for Quantitative Analysis of Medicinal Plant Extracts and Herbal Products." Phytochemical Analysis : PCA, vol. 23, no. 6, 2012, pp. 689-96.
Chauthe SK, Sharma RJ, Aqil F, et al. Quantitative NMR: an applicable method for quantitative analysis of medicinal plant extracts and herbal products. Phytochem Anal. 2012;23(6):689-96.
Chauthe, S. K., Sharma, R. J., Aqil, F., Gupta, R. C., & Singh, I. P. (2012). Quantitative NMR: an applicable method for quantitative analysis of medicinal plant extracts and herbal products. Phytochemical Analysis : PCA, 23(6), 689-96. https://doi.org/10.1002/pca.2375
Chauthe SK, et al. Quantitative NMR: an Applicable Method for Quantitative Analysis of Medicinal Plant Extracts and Herbal Products. Phytochem Anal. 2012 Nov-Dec;23(6):689-96. PubMed PMID: 22707000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative NMR: an applicable method for quantitative analysis of medicinal plant extracts and herbal products. AU - Chauthe,Siddheshwar K, AU - Sharma,Ram Jee, AU - Aqil,Farrukh, AU - Gupta,Ramesh C, AU - Singh,Inder Pal, Y1 - 2012/06/15/ PY - 2012/02/28/received PY - 2012/04/11/revised PY - 2012/04/24/accepted PY - 2012/6/19/entrez PY - 2012/6/19/pubmed PY - 2013/4/27/medline SP - 689 EP - 96 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 23 IS - 6 N2 - INTRODUCTION: Quantitative analysis and standardisation of plant extracts or herbal products is a tedious process requiring time-consuming sample preparation and analytical method development for the resolution of analyte peaks from the complex natural extract. Quantitative analysis by HPLC requires a pure authentic standard of the compound being quantified. We report here a quantitative NMR (qNMR) method for quantitative analysis of three medicinal plant extracts and their herbal products without the need of authentic standards. Quantitation can be done by using any commercially available pure sample as an internal reference standard. OBJECTIVE: To develop a reliable method for standardisation and quantitative analysis of extracts from medicinal plants Eugenia jambolana, Withania somnifera and Aegle marmelos and their herbal products using qNMR. METHODOLOGY: The (1) H-NMR spectra of known amounts of crude plant extracts with internal standards were recorded in deuterated solvents and quantitation was performed by calculating the relative ratio of the peak area of selected proton signals of the target compounds and the internal reference standard. Anthocyanins [delphinidin-3,5-diglucoside (1), petunidin-3,5-diglucoside (2) and malvidin-3,5-diglucoside (3)] for E. jambolana fruit extract and imperatorin (4) for A. marmelos fruit extract were selected as marker constituents for quantitation and 1,3,5-trimethoxybenzene (TMB) was used as an internal reference standard. Total withanolide content was determined for W. somnifera using 2,4-diformyl phloroglucinol as an internal reference standard. RESULTS: The (1) H-NMR gave a linear response for the marker constituents, anthocyanins, withaferin A and imperatorin. Using the described method, the amount of anthocyanins in Amberlite(R) XAD7HP and Sephadex enriched extracts of E. jambolana was 3.77% and 9.57% (delphinidin-3,5-diglucoside), 4.72% and 12.0% (petunidin-3,5-diglucoside), 6.55% and 15.70% (malvidin-3,5-diglucoside), respectively. The imperatorin content was 0.424% in A. marmelos fruit and 0.090 % and 0.114% in sharbat and candies. Total withanolides content was 0.191% in the chloroform extract and 0.234% in the capsule extract. These values are in accordance with HPLC results. CONCLUSION: This qNMR technique could be used for NMR fingerprinting and quantitation for the purpose of quality control and standardisation of many plant-based herbal products and medicines and has certain advantages over HPLC. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/22707000/Quantitative_NMR:_an_applicable_method_for_quantitative_analysis_of_medicinal_plant_extracts_and_herbal_products_ L2 - https://doi.org/10.1002/pca.2375 DB - PRIME DP - Unbound Medicine ER -