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Biochemical and genotoxic effect of triclosan on earthworms (Eisenia fetida) using contact and soil tests.
Environ Toxicol. 2012 Jul; 27(7):385-92.ET

Abstract

Triclosan (TCS) is a broad-spectrum bactericide that is used for a variety of antimicrobial functions. TCS is frequently detected in the terrestrial environment due to application of sewage sludge to agricultural land. In the present study, 48-h paper contact and 28-day spiked soil tests were conducted to examine the toxic effects of TCS on the antioxidative and genetic indices of earthworms (Eisenia fetida). The activity of antioxidative enzymes (superoxide dismutase, SOD; catalase, CAT) and the content of the lipid peroxidation product (malondialdehyde, MDA) were determined as biomarkers of oxidative stress in E. fetida. Moreover, single cell gel electrophoresis (SCGE) was used as a biomarker of genotoxicity. The results showed that triclosan induced a significant increase (P < 0.05) in antioxidative enzyme activities and MDA content. Of all of the biomarkers examined, CAT activity was most sensitive to TCS, and the CAT activity increased significantly (P < 0.05) at bactericidal concentrations of 7.86 ng cm⁻² in the contact test and 10 mg kg⁻¹ in the spiked soil test. The comet assay showed that TCS treatments significantly induced (P < 0.05) DNA damage in E. fetida, and that 78.6 ng cm⁻² caused significant genotoxic effects in the acute test (48 h). Clear dose-dependent DNA damage to E. fetida was observed both in contact and spiked soil tests. These results imply that TCS may have potential biochemical and genetic toxicity toward earthworms (E. fetida). A battery of biomarkers covering multiple molecular targets of acute toxicity can be combined to better understand the impacts of TCS on E. fetida.

Authors+Show Affiliations

Key Laboratory of Pollution Process and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22707219

Citation

Lin, Dasong, et al. "Biochemical and Genotoxic Effect of Triclosan On Earthworms (Eisenia Fetida) Using Contact and Soil Tests." Environmental Toxicology, vol. 27, no. 7, 2012, pp. 385-92.
Lin D, Xie X, Zhou Q, et al. Biochemical and genotoxic effect of triclosan on earthworms (Eisenia fetida) using contact and soil tests. Environ Toxicol. 2012;27(7):385-92.
Lin, D., Xie, X., Zhou, Q., & Liu, Y. (2012). Biochemical and genotoxic effect of triclosan on earthworms (Eisenia fetida) using contact and soil tests. Environmental Toxicology, 27(7), 385-92. https://doi.org/10.1002/tox.20651
Lin D, et al. Biochemical and Genotoxic Effect of Triclosan On Earthworms (Eisenia Fetida) Using Contact and Soil Tests. Environ Toxicol. 2012;27(7):385-92. PubMed PMID: 22707219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical and genotoxic effect of triclosan on earthworms (Eisenia fetida) using contact and soil tests. AU - Lin,Dasong, AU - Xie,Xiujie, AU - Zhou,Qixing, AU - Liu,Yao, Y1 - 2010/12/08/ PY - 2010/04/25/received PY - 2010/08/04/revised PY - 2010/08/10/accepted PY - 2012/6/19/entrez PY - 2012/6/19/pubmed PY - 2013/2/26/medline SP - 385 EP - 92 JF - Environmental toxicology JO - Environ Toxicol VL - 27 IS - 7 N2 - Triclosan (TCS) is a broad-spectrum bactericide that is used for a variety of antimicrobial functions. TCS is frequently detected in the terrestrial environment due to application of sewage sludge to agricultural land. In the present study, 48-h paper contact and 28-day spiked soil tests were conducted to examine the toxic effects of TCS on the antioxidative and genetic indices of earthworms (Eisenia fetida). The activity of antioxidative enzymes (superoxide dismutase, SOD; catalase, CAT) and the content of the lipid peroxidation product (malondialdehyde, MDA) were determined as biomarkers of oxidative stress in E. fetida. Moreover, single cell gel electrophoresis (SCGE) was used as a biomarker of genotoxicity. The results showed that triclosan induced a significant increase (P < 0.05) in antioxidative enzyme activities and MDA content. Of all of the biomarkers examined, CAT activity was most sensitive to TCS, and the CAT activity increased significantly (P < 0.05) at bactericidal concentrations of 7.86 ng cm⁻² in the contact test and 10 mg kg⁻¹ in the spiked soil test. The comet assay showed that TCS treatments significantly induced (P < 0.05) DNA damage in E. fetida, and that 78.6 ng cm⁻² caused significant genotoxic effects in the acute test (48 h). Clear dose-dependent DNA damage to E. fetida was observed both in contact and spiked soil tests. These results imply that TCS may have potential biochemical and genetic toxicity toward earthworms (E. fetida). A battery of biomarkers covering multiple molecular targets of acute toxicity can be combined to better understand the impacts of TCS on E. fetida. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/22707219/Biochemical_and_genotoxic_effect_of_triclosan_on_earthworms__Eisenia_fetida__using_contact_and_soil_tests_ L2 - https://doi.org/10.1002/tox.20651 DB - PRIME DP - Unbound Medicine ER -