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Association mapping in sunflower for Sclerotinia Head Rot resistance.
BMC Plant Biol. 2012 Jun 18; 12:93.BP

Abstract

BACKGROUND

Sclerotinia Head Rot (SHR) is one of the most damaging diseases of sunflower in Europe, Argentina, and USA, causing average yield reductions of 10 to 20 %, but leading to total production loss under favorable environmental conditions for the pathogen. Association Mapping (AM) is a promising choice for Quantitative Trait Locus (QTL) mapping, as it detects relationships between phenotypic variation and gene polymorphisms in existing germplasm without development of mapping populations. This article reports the identification of QTL for resistance to SHR based on candidate gene AM.

RESULTS

A collection of 94 sunflower inbred lines were tested for SHR under field conditions using assisted inoculation with the fungal pathogen Sclerotinia sclerotiorum. Given that no biological mechanisms or biochemical pathways have been clearly identified for SHR, 43 candidate genes were selected based on previous transcript profiling studies in sunflower and Brassica napus infected with S. sclerotiorum. Associations among SHR incidence and haplotype polymorphisms in 16 candidate genes were tested using Mixed Linear Models (MLM) that account for population structure and kinship relationships. This approach allowed detection of a significant association between the candidate gene HaRIC_B and SHR incidence (P < 0.01), accounting for a SHR incidence reduction of about 20 %.

CONCLUSIONS

These results suggest that AM will be useful in dissecting other complex traits in sunflower, thus providing a valuable tool to assist in crop breeding.

Authors+Show Affiliations

Instituto de Biotecnología, Centro Investigación en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22708963

Citation

Fusari, Corina M., et al. "Association Mapping in Sunflower for Sclerotinia Head Rot Resistance." BMC Plant Biology, vol. 12, 2012, p. 93.
Fusari CM, Di Rienzo JA, Troglia C, et al. Association mapping in sunflower for Sclerotinia Head Rot resistance. BMC Plant Biol. 2012;12:93.
Fusari, C. M., Di Rienzo, J. A., Troglia, C., Nishinakamasu, V., Moreno, M. V., Maringolo, C., Quiroz, F., Alvarez, D., Escande, A., Hopp, E., Heinz, R., Lia, V. V., & Paniego, N. B. (2012). Association mapping in sunflower for Sclerotinia Head Rot resistance. BMC Plant Biology, 12, 93. https://doi.org/10.1186/1471-2229-12-93
Fusari CM, et al. Association Mapping in Sunflower for Sclerotinia Head Rot Resistance. BMC Plant Biol. 2012 Jun 18;12:93. PubMed PMID: 22708963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association mapping in sunflower for Sclerotinia Head Rot resistance. AU - Fusari,Corina M, AU - Di Rienzo,Julio A, AU - Troglia,Carolina, AU - Nishinakamasu,Verónica, AU - Moreno,María Valeria, AU - Maringolo,Carla, AU - Quiroz,Facundo, AU - Alvarez,Daniel, AU - Escande,Alberto, AU - Hopp,Esteban, AU - Heinz,Ruth, AU - Lia,Verónica V, AU - Paniego,Norma B, Y1 - 2012/06/18/ PY - 2011/11/30/received PY - 2012/05/21/accepted PY - 2012/6/20/entrez PY - 2012/6/20/pubmed PY - 2014/8/27/medline SP - 93 EP - 93 JF - BMC plant biology JO - BMC Plant Biol. VL - 12 N2 - BACKGROUND: Sclerotinia Head Rot (SHR) is one of the most damaging diseases of sunflower in Europe, Argentina, and USA, causing average yield reductions of 10 to 20 %, but leading to total production loss under favorable environmental conditions for the pathogen. Association Mapping (AM) is a promising choice for Quantitative Trait Locus (QTL) mapping, as it detects relationships between phenotypic variation and gene polymorphisms in existing germplasm without development of mapping populations. This article reports the identification of QTL for resistance to SHR based on candidate gene AM. RESULTS: A collection of 94 sunflower inbred lines were tested for SHR under field conditions using assisted inoculation with the fungal pathogen Sclerotinia sclerotiorum. Given that no biological mechanisms or biochemical pathways have been clearly identified for SHR, 43 candidate genes were selected based on previous transcript profiling studies in sunflower and Brassica napus infected with S. sclerotiorum. Associations among SHR incidence and haplotype polymorphisms in 16 candidate genes were tested using Mixed Linear Models (MLM) that account for population structure and kinship relationships. This approach allowed detection of a significant association between the candidate gene HaRIC_B and SHR incidence (P < 0.01), accounting for a SHR incidence reduction of about 20 %. CONCLUSIONS: These results suggest that AM will be useful in dissecting other complex traits in sunflower, thus providing a valuable tool to assist in crop breeding. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/22708963/Association_mapping_in_sunflower_for_Sclerotinia_Head_Rot_resistance_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-12-93 DB - PRIME DP - Unbound Medicine ER -