Tags

Type your tag names separated by a space and hit enter

Antioxidant effect of silymarin on paraquat-induced human lung adenocarcinoma A549 cell line.
Food Chem Toxicol. 2012 Sep; 50(9):3206-14.FC

Abstract

Paraquat (PQ) is not only widely used as a potent herbicide but also causes severe fatality to humans around the world due to accidental or intentional ingestion. Silymarin is a well-known phytochemical whose multi-functional effects in humans include anti-oxidant, anti-inflammatory and anti-cancer activities. The efficacy of silymarin in protecting against PQ-induced cytotoxicity is unknown. This study investigated the potential role of silymarin against PQ-induced oxidative stress on human A549 adenocarcinoma cell line. Colorimetric-based viability assay, determination of reactive oxygen species, cell damage assay based on lactate dehydrogense retention, anti-oxidant enzyme assay, Western blot and quantitative reverse transcription-polymerase chain reaction analyses were done. Our data revealed that silymarin could dramatically prevent cell toxicity, and reduce the LDH retention induced by PQ on A549 cell line. Silymarin acted as a potent cytoprotective effector through the effective induction of anti-oxidant related genes, including Nrf2, NQO1 and HO-1, in the presence of PQ. The induction of the Nrf2, HO-1 and NQO1 genes was first evident after 3h. The data indicate the potential of silymarin in alleviating PQ intoxication.

Authors+Show Affiliations

Department of Microbiology, School of Medicine, Soonchunhyang University Cheonan, Chungnam 330-090, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22709784

Citation

Podder, Biswajit, et al. "Antioxidant Effect of Silymarin On Paraquat-induced Human Lung Adenocarcinoma A549 Cell Line." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 50, no. 9, 2012, pp. 3206-14.
Podder B, Kim YS, Zerin T, et al. Antioxidant effect of silymarin on paraquat-induced human lung adenocarcinoma A549 cell line. Food Chem Toxicol. 2012;50(9):3206-14.
Podder, B., Kim, Y. S., Zerin, T., & Song, H. Y. (2012). Antioxidant effect of silymarin on paraquat-induced human lung adenocarcinoma A549 cell line. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 50(9), 3206-14. https://doi.org/10.1016/j.fct.2012.06.007
Podder B, et al. Antioxidant Effect of Silymarin On Paraquat-induced Human Lung Adenocarcinoma A549 Cell Line. Food Chem Toxicol. 2012;50(9):3206-14. PubMed PMID: 22709784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant effect of silymarin on paraquat-induced human lung adenocarcinoma A549 cell line. AU - Podder,Biswajit, AU - Kim,Yong-Sik, AU - Zerin,Tamanna, AU - Song,Ho-Yeon, Y1 - 2012/06/16/ PY - 2012/01/30/received PY - 2012/06/04/revised PY - 2012/06/07/accepted PY - 2012/6/20/entrez PY - 2012/6/20/pubmed PY - 2013/1/16/medline SP - 3206 EP - 14 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 50 IS - 9 N2 - Paraquat (PQ) is not only widely used as a potent herbicide but also causes severe fatality to humans around the world due to accidental or intentional ingestion. Silymarin is a well-known phytochemical whose multi-functional effects in humans include anti-oxidant, anti-inflammatory and anti-cancer activities. The efficacy of silymarin in protecting against PQ-induced cytotoxicity is unknown. This study investigated the potential role of silymarin against PQ-induced oxidative stress on human A549 adenocarcinoma cell line. Colorimetric-based viability assay, determination of reactive oxygen species, cell damage assay based on lactate dehydrogense retention, anti-oxidant enzyme assay, Western blot and quantitative reverse transcription-polymerase chain reaction analyses were done. Our data revealed that silymarin could dramatically prevent cell toxicity, and reduce the LDH retention induced by PQ on A549 cell line. Silymarin acted as a potent cytoprotective effector through the effective induction of anti-oxidant related genes, including Nrf2, NQO1 and HO-1, in the presence of PQ. The induction of the Nrf2, HO-1 and NQO1 genes was first evident after 3h. The data indicate the potential of silymarin in alleviating PQ intoxication. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/22709784/Antioxidant_effect_of_silymarin_on_paraquat_induced_human_lung_adenocarcinoma_A549_cell_line_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(12)00421-8 DB - PRIME DP - Unbound Medicine ER -