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[Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells].
Wei Sheng Wu Xue Bao. 2012 Mar 04; 52(3):318-25.WS

Abstract

OBJECTIVE

To investigate whether the recombinant baculovirus BV-T7 hybrid expression system can be effectively transduced into chicken cells in vitro, and whether it can express the foreign genes (eGFP).

METHOD

We established a hybrid baculovirus-T7 RNA polymerase system for transient expression in mammalian cells and chicken cells. Two recombinant baculoviruses were constructed, one carrying cDNA of bacteriophage T7 RNA polymerase, with a nuclear localization signal, under the control of a mammalian promoter and the other expressing eGFP gene under the control of T7 promoter. The constructed recombinant baculoviruses co-infected mammalian oligodendrocyte cells, as well as chicken embryo fibroblasts cells and chicken embryo skeletal muscle cells.

RESULTS

The eGFP activity was detected in mammalian cell lines and embryo fibroblasts cells and chicken embryo skeletal muscle cells. The recombinant baculovirus transduction efficiency of oligodendrocyte cells was 59.5%, and in CEF cells and myoblast cells the transduction efficiencies were 23.2% and 33.1%.

CONCLUSION

BV-T7 hybrid expression could be expressed T7 RNAP in mammalian cells and avian cells.

Authors+Show Affiliations

Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China. gejingping@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22712402

Citation

Ge, Jingping, et al. "[Construction of BV-T7 Hybrid Expression System Based On Baculovirus to Express Target Gene eGFP in Mammalian and Chicken Cells]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 52, no. 3, 2012, pp. 318-25.
Ge J, Tang X, Gao D, et al. [Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells]. Wei Sheng Wu Xue Bao. 2012;52(3):318-25.
Ge, J., Tang, X., Gao, D., Song, S., Lu, S., Lou, Z., & Ping, W. (2012). [Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 52(3), 318-25.
Ge J, et al. [Construction of BV-T7 Hybrid Expression System Based On Baculovirus to Express Target Gene eGFP in Mammalian and Chicken Cells]. Wei Sheng Wu Xue Bao. 2012 Mar 4;52(3):318-25. PubMed PMID: 22712402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells]. AU - Ge,Jingping, AU - Tang,Xiaoyan, AU - Gao,Dongni, AU - Song,Shanshan, AU - Lu,Shan, AU - Lou,Zhuangwei, AU - Ping,Wenxiang, PY - 2012/6/21/entrez PY - 2012/6/21/pubmed PY - 2012/7/18/medline SP - 318 EP - 25 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 52 IS - 3 N2 - OBJECTIVE: To investigate whether the recombinant baculovirus BV-T7 hybrid expression system can be effectively transduced into chicken cells in vitro, and whether it can express the foreign genes (eGFP). METHOD: We established a hybrid baculovirus-T7 RNA polymerase system for transient expression in mammalian cells and chicken cells. Two recombinant baculoviruses were constructed, one carrying cDNA of bacteriophage T7 RNA polymerase, with a nuclear localization signal, under the control of a mammalian promoter and the other expressing eGFP gene under the control of T7 promoter. The constructed recombinant baculoviruses co-infected mammalian oligodendrocyte cells, as well as chicken embryo fibroblasts cells and chicken embryo skeletal muscle cells. RESULTS: The eGFP activity was detected in mammalian cell lines and embryo fibroblasts cells and chicken embryo skeletal muscle cells. The recombinant baculovirus transduction efficiency of oligodendrocyte cells was 59.5%, and in CEF cells and myoblast cells the transduction efficiencies were 23.2% and 33.1%. CONCLUSION: BV-T7 hybrid expression could be expressed T7 RNAP in mammalian cells and avian cells. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/22712402/[Construction_of_BV_T7_hybrid_expression_system_based_on_baculovirus_to_express_target_gene_eGFP_in_mammalian_and_chicken_cells]_ DB - PRIME DP - Unbound Medicine ER -