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Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens.
Clin Exp Ophthalmol. 2013 Mar; 41(2):172-9.CE

Abstract

BACKGROUND

To investigate the preventive effect of danshensu on the selenite-induced opacification of cultured rat lenses.

METHODS

Isolated lens were divided into three groups with eight lenses in each group. Group I: lenses were incubated with M199 medium alone; Group II: incubated in M199 containing 200 µmol/L sodium selenite; Group III: incubated in M199 containing 200 µmol/L sodium selenite and 500 µmol/L danshensu. Selenite was administered on the third day, and danshensu treatment was from the second to the fifth day. Cataracts development was observed using an inverted microscope, and the lenses were analysed for total anti-oxidative capabilities, mean activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase; levels of reduced glutathione; malondialdehyde; and total sulfhydryl content.

RESULTS

All lenses in Group I were clear, whereas all lenses in Group II developed dense vacuolization and opacification. In Group III, 25% lenses revealed minimal vacuolization, and 75% showed no opacification or vacuolization. Total anti-oxidative capabilities and the mean activities of anti-oxidant enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase; levels of glutathione; and total sulfhydryl content were elevated, and the level of malondialdehyde was decreased following treatment with danshensu compared with Group II.

CONCLUSION

The anti-oxidative properties of danshensu may play a major role in its contribution to the anticataract effect.

Authors+Show Affiliations

Departments of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22712555

Citation

Qi, Hui-Ping, et al. "Preventive Effect of Danshensu On Selenite-induced Cataractogenesis in Cultured Rat Lens." Clinical & Experimental Ophthalmology, vol. 41, no. 2, 2013, pp. 172-9.
Qi HP, Wei SQ, Zhang LQ, et al. Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens. Clin Exp Ophthalmol. 2013;41(2):172-9.
Qi, H. P., Wei, S. Q., Zhang, L. Q., Gao, X. C., Yu, N. N., Bi, S., & Cui, H. (2013). Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens. Clinical & Experimental Ophthalmology, 41(2), 172-9. https://doi.org/10.1111/j.1442-9071.2012.02837.x
Qi HP, et al. Preventive Effect of Danshensu On Selenite-induced Cataractogenesis in Cultured Rat Lens. Clin Exp Ophthalmol. 2013;41(2):172-9. PubMed PMID: 22712555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens. AU - Qi,Hui-Ping, AU - Wei,Shu-Qin, AU - Zhang,Li-Qiong, AU - Gao,Xiang-Chun, AU - Yu,Nan-Nan, AU - Bi,Sheng, AU - Cui,Hao, Y1 - 2012/09/04/ PY - 2012/6/21/entrez PY - 2012/6/21/pubmed PY - 2013/9/10/medline SP - 172 EP - 9 JF - Clinical & experimental ophthalmology JO - Clin Exp Ophthalmol VL - 41 IS - 2 N2 - BACKGROUND: To investigate the preventive effect of danshensu on the selenite-induced opacification of cultured rat lenses. METHODS: Isolated lens were divided into three groups with eight lenses in each group. Group I: lenses were incubated with M199 medium alone; Group II: incubated in M199 containing 200 µmol/L sodium selenite; Group III: incubated in M199 containing 200 µmol/L sodium selenite and 500 µmol/L danshensu. Selenite was administered on the third day, and danshensu treatment was from the second to the fifth day. Cataracts development was observed using an inverted microscope, and the lenses were analysed for total anti-oxidative capabilities, mean activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase; levels of reduced glutathione; malondialdehyde; and total sulfhydryl content. RESULTS: All lenses in Group I were clear, whereas all lenses in Group II developed dense vacuolization and opacification. In Group III, 25% lenses revealed minimal vacuolization, and 75% showed no opacification or vacuolization. Total anti-oxidative capabilities and the mean activities of anti-oxidant enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase; levels of glutathione; and total sulfhydryl content were elevated, and the level of malondialdehyde was decreased following treatment with danshensu compared with Group II. CONCLUSION: The anti-oxidative properties of danshensu may play a major role in its contribution to the anticataract effect. SN - 1442-9071 UR - https://www.unboundmedicine.com/medline/citation/22712555/Preventive_effect_of_danshensu_on_selenite_induced_cataractogenesis_in_cultured_rat_lens_ DB - PRIME DP - Unbound Medicine ER -