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Enzyme inhibitory and antioxidant activities of traditional medicinal plants: potential application in the management of hyperglycemia.
BMC Complement Altern Med. 2012 Jun 19; 12:77.BC

Abstract

BACKGROUND

Traditional Indian and Australian medicinal plant extracts were investigated to determine their therapeutic potential to inhibit key enzymes in carbohydrate metabolism, which has relevance to the management of hyperglycemia and type 2 diabetes. The antioxidant activities were also assessed.

METHODS

The evaluation of enzyme inhibitory activity of seven Australian aboriginal medicinal plants and five Indian Ayurvedic plants was carried out against α-amylase and α-glucosidase. Antioxidant activity was determined by measuring (i) the scavenging effect of plant extracts against 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) and (ii) ferric reducing power. Total phenolic and total flavonoid contents were also determined.

RESULTS

Of the twelve plant extracts evaluated, the highest inhibitory activity against both α-amylase and α-glucosidase enzymes was exerted by Santalum spicatum and Pterocarpus marsupium with IC50 values of 5.43 μg/ml and 0.9 μg/ml, respectively, and 5.16 μg/ml and 1.06 μg/ml, respectively. However, the extracts of Acacia ligulata (IC50 = 1.01 μg/ml), Beyeria leshnaultii (0.39 μg/ml), Mucuna pruriens (0.8 μg/ml) and Boerhaavia diffusa (1.72 μg/ml) exhibited considerable activity against α-glucosidase enzyme only. The free radical scavenging activity was found to be prominent in extracts of Acacia kempeana, Acacia ligulata followed by Euphorbia drummondii against both DPPH and ABTS. The reducing power was more pronounced in Euphorbia drummondii and Pterocarpus marsupium extracts. The phenolic and flavonoid contents ranged from 0.42 to 30.27 μg/mg equivalent of gallic acid and 0.51 to 32.94 μg/mg equivalent of quercetin, respectively, in all plant extracts. Pearson's correlation coefficient between total flavonoids and total phenolics was 0.796.

CONCLUSION

The results obtained in this study showed that most of the plant extracts have good potential for the management of hyperglycemia, diabetes and the related condition of oxidative stress.

Authors+Show Affiliations

Environment and Biotechnology Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122 VIC, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22713130

Citation

Gulati, Vandana, et al. "Enzyme Inhibitory and Antioxidant Activities of Traditional Medicinal Plants: Potential Application in the Management of Hyperglycemia." BMC Complementary and Alternative Medicine, vol. 12, 2012, p. 77.
Gulati V, Harding IH, Palombo EA. Enzyme inhibitory and antioxidant activities of traditional medicinal plants: potential application in the management of hyperglycemia. BMC Complement Altern Med. 2012;12:77.
Gulati, V., Harding, I. H., & Palombo, E. A. (2012). Enzyme inhibitory and antioxidant activities of traditional medicinal plants: potential application in the management of hyperglycemia. BMC Complementary and Alternative Medicine, 12, 77. https://doi.org/10.1186/1472-6882-12-77
Gulati V, Harding IH, Palombo EA. Enzyme Inhibitory and Antioxidant Activities of Traditional Medicinal Plants: Potential Application in the Management of Hyperglycemia. BMC Complement Altern Med. 2012 Jun 19;12:77. PubMed PMID: 22713130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme inhibitory and antioxidant activities of traditional medicinal plants: potential application in the management of hyperglycemia. AU - Gulati,Vandana, AU - Harding,Ian H, AU - Palombo,Enzo A, Y1 - 2012/06/19/ PY - 2011/08/15/received PY - 2012/06/07/accepted PY - 2012/6/21/entrez PY - 2012/6/21/pubmed PY - 2013/5/23/medline SP - 77 EP - 77 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 12 N2 - BACKGROUND: Traditional Indian and Australian medicinal plant extracts were investigated to determine their therapeutic potential to inhibit key enzymes in carbohydrate metabolism, which has relevance to the management of hyperglycemia and type 2 diabetes. The antioxidant activities were also assessed. METHODS: The evaluation of enzyme inhibitory activity of seven Australian aboriginal medicinal plants and five Indian Ayurvedic plants was carried out against α-amylase and α-glucosidase. Antioxidant activity was determined by measuring (i) the scavenging effect of plant extracts against 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) and (ii) ferric reducing power. Total phenolic and total flavonoid contents were also determined. RESULTS: Of the twelve plant extracts evaluated, the highest inhibitory activity against both α-amylase and α-glucosidase enzymes was exerted by Santalum spicatum and Pterocarpus marsupium with IC50 values of 5.43 μg/ml and 0.9 μg/ml, respectively, and 5.16 μg/ml and 1.06 μg/ml, respectively. However, the extracts of Acacia ligulata (IC50 = 1.01 μg/ml), Beyeria leshnaultii (0.39 μg/ml), Mucuna pruriens (0.8 μg/ml) and Boerhaavia diffusa (1.72 μg/ml) exhibited considerable activity against α-glucosidase enzyme only. The free radical scavenging activity was found to be prominent in extracts of Acacia kempeana, Acacia ligulata followed by Euphorbia drummondii against both DPPH and ABTS. The reducing power was more pronounced in Euphorbia drummondii and Pterocarpus marsupium extracts. The phenolic and flavonoid contents ranged from 0.42 to 30.27 μg/mg equivalent of gallic acid and 0.51 to 32.94 μg/mg equivalent of quercetin, respectively, in all plant extracts. Pearson's correlation coefficient between total flavonoids and total phenolics was 0.796. CONCLUSION: The results obtained in this study showed that most of the plant extracts have good potential for the management of hyperglycemia, diabetes and the related condition of oxidative stress. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/22713130/Enzyme_inhibitory_and_antioxidant_activities_of_traditional_medicinal_plants:_potential_application_in_the_management_of_hyperglycemia_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-12-77 DB - PRIME DP - Unbound Medicine ER -