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Effect of acid and base catalyzed hydrolysis on the yield of phenolics and antioxidant activity of extracts from germinated brown rice (GBR).
Molecules. 2012 Jun 19; 17(6):7584-94.M

Abstract

The influence of both acidic and basic hydrolysis on the yield, total phenolic content and antioxidative capacity of methanolic extract of germinated brown rice (GBR) was studied. Total phenolic content (TPC), total flavonoid content (TFC), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging, and ferric reducing antioxidant power (FRAP) tests were used for the measurement of antioxidant ability. There was a significant difference p < 0.05) in the TPC and DPPH radical scavenging assay results when comparing neutral with acidic and basic catalysed hydrolysis. The yield of the crude extract was slightly higher in acidic hydrolysis than in basic hydrolysis p > 0.05). The TPC and TFC were highest in acidic hydrolysis. A significant correlation was observed between ABTS radical cation scavenging and FRAP. The antioxidant activity measured using DPPH radical scavenging assay showed high activity in acidic hydrolysis, while the ABTS radical cationscavenging activity and FRAP showed the highest values in basic hydrolysis. The samples were further evaluated using HPLC to determine the individual phenolic concentrations in different hydrolytic media contributing to the antioxidant effects. This study revealed that acidic and basic hydrolysis can improve the yield, phenolic content, and antioxidant activity of germinated brown rice.

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Authors+Show Affiliations

Sani IM
Laboratory of Molecular Biomedicine, Institute of Bioscience, UniversitiPutra Malaysia, Serdang 43400, Selangor, Malaysia.
Iqbal S
No affiliation info available
Chan KW
No affiliation info available
Ismail M
No affiliation info available

MeSH

AcidsAntioxidantsCatalysisFlavonoidsFree Radical ScavengersGerminationHydrolysisOryzaPhenolsPlant Extracts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22713349

Citation

Sani, Ismaila Muhammad, et al. "Effect of Acid and Base Catalyzed Hydrolysis On the Yield of Phenolics and Antioxidant Activity of Extracts From Germinated Brown Rice (GBR)." Molecules (Basel, Switzerland), vol. 17, no. 6, 2012, pp. 7584-94.
Sani IM, Iqbal S, Chan KW, et al. Effect of acid and base catalyzed hydrolysis on the yield of phenolics and antioxidant activity of extracts from germinated brown rice (GBR). Molecules. 2012;17(6):7584-94.
Sani, I. M., Iqbal, S., Chan, K. W., & Ismail, M. (2012). Effect of acid and base catalyzed hydrolysis on the yield of phenolics and antioxidant activity of extracts from germinated brown rice (GBR). Molecules (Basel, Switzerland), 17(6), 7584-94. https://doi.org/10.3390/molecules17067584
Sani IM, et al. Effect of Acid and Base Catalyzed Hydrolysis On the Yield of Phenolics and Antioxidant Activity of Extracts From Germinated Brown Rice (GBR). Molecules. 2012 Jun 19;17(6):7584-94. PubMed PMID: 22713349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of acid and base catalyzed hydrolysis on the yield of phenolics and antioxidant activity of extracts from germinated brown rice (GBR). AU - Sani,Ismaila Muhammad, AU - Iqbal,Shahid, AU - Chan,Kim Wei, AU - Ismail,Maznah, Y1 - 2012/06/19/ PY - 2012/05/01/received PY - 2012/05/21/revised PY - 2012/05/25/accepted PY - 2012/6/21/entrez PY - 2012/6/21/pubmed PY - 2012/10/10/medline SP - 7584 EP - 94 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 17 IS - 6 N2 - The influence of both acidic and basic hydrolysis on the yield, total phenolic content and antioxidative capacity of methanolic extract of germinated brown rice (GBR) was studied. Total phenolic content (TPC), total flavonoid content (TFC), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging, and ferric reducing antioxidant power (FRAP) tests were used for the measurement of antioxidant ability. There was a significant difference p < 0.05) in the TPC and DPPH radical scavenging assay results when comparing neutral with acidic and basic catalysed hydrolysis. The yield of the crude extract was slightly higher in acidic hydrolysis than in basic hydrolysis p > 0.05). The TPC and TFC were highest in acidic hydrolysis. A significant correlation was observed between ABTS radical cation scavenging and FRAP. The antioxidant activity measured using DPPH radical scavenging assay showed high activity in acidic hydrolysis, while the ABTS radical cationscavenging activity and FRAP showed the highest values in basic hydrolysis. The samples were further evaluated using HPLC to determine the individual phenolic concentrations in different hydrolytic media contributing to the antioxidant effects. This study revealed that acidic and basic hydrolysis can improve the yield, phenolic content, and antioxidant activity of germinated brown rice. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/22713349/Effect_of_acid_and_base_catalyzed_hydrolysis_on_the_yield_of_phenolics_and_antioxidant_activity_of_extracts_from_germinated_brown_rice__GBR__ DB - PRIME DP - Unbound Medicine ER -