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Maturational regression of glomeruli determines the nephron population in normal mice.
Pediatr Res 2012; 72(3):241-8PR

Abstract

BACKGROUND

Regression is an important process in the normal development of many organs. In this study, we investigated whether glomerular regression occurs after normal glomerulogenesis and determined the time course for this process.

METHODS

Glomerular number was analyzed in normal mouse kidneys at postnatal day (P)7, P10, P14, P18, P21, P25, and P28 by the gold standard fractionator/dissector method, which involves exhausting the kidney tissue. Vascular regression markers, angiopoietin 2 (ANGPT2), and thrombospondin 1 (THBS1), were examined by immunohistochemistry.

RESULTS

The maximum glomerular number was reached at P7 with 14,051 glomeruli per kidney (95% confidence interval: 12,084-16,018). This peak was followed by a progressive reduction, with a nadir of 11,060 (10,393-11,727) occurring at P18 (P < 0.05 as compared with P7). Thereafter, glomerular number remained constant. Complementary immunohistochemical examination of vascular regression markers showed peak expression of glomerular ANGPT2 and THBS1 at P14.

CONCLUSION

Our study reveals that the tissue- and time-saving Weibel-Gomez method commonly used to assess glomerular number is valid only after P18. The data indicate that regulation of glomerular number by regression occurs in normally maturing mouse kidneys. These findings suggest that the process of glomerular regression could be therapeutically targeted to prevent oligonephronia, which otherwise predisposes to chronic kidney disease.

Authors+Show Affiliations

Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22717689

Citation

Zhong, Jianyong, et al. "Maturational Regression of Glomeruli Determines the Nephron Population in Normal Mice." Pediatric Research, vol. 72, no. 3, 2012, pp. 241-8.
Zhong J, Perrien DS, Yang HC, et al. Maturational regression of glomeruli determines the nephron population in normal mice. Pediatr Res. 2012;72(3):241-8.
Zhong, J., Perrien, D. S., Yang, H. C., Kon, V., Fogo, A. B., Ichikawa, I., & Ma, J. (2012). Maturational regression of glomeruli determines the nephron population in normal mice. Pediatric Research, 72(3), pp. 241-8. doi:10.1038/pr.2012.81.
Zhong J, et al. Maturational Regression of Glomeruli Determines the Nephron Population in Normal Mice. Pediatr Res. 2012;72(3):241-8. PubMed PMID: 22717689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maturational regression of glomeruli determines the nephron population in normal mice. AU - Zhong,Jianyong, AU - Perrien,Daniel Scott, AU - Yang,Hai-Chun, AU - Kon,Valentina, AU - Fogo,Agnes B, AU - Ichikawa,Iekuni, AU - Ma,Ji, Y1 - 2012/06/20/ PY - 2012/6/22/entrez PY - 2012/6/22/pubmed PY - 2012/12/29/medline SP - 241 EP - 8 JF - Pediatric research JO - Pediatr. Res. VL - 72 IS - 3 N2 - BACKGROUND: Regression is an important process in the normal development of many organs. In this study, we investigated whether glomerular regression occurs after normal glomerulogenesis and determined the time course for this process. METHODS: Glomerular number was analyzed in normal mouse kidneys at postnatal day (P)7, P10, P14, P18, P21, P25, and P28 by the gold standard fractionator/dissector method, which involves exhausting the kidney tissue. Vascular regression markers, angiopoietin 2 (ANGPT2), and thrombospondin 1 (THBS1), were examined by immunohistochemistry. RESULTS: The maximum glomerular number was reached at P7 with 14,051 glomeruli per kidney (95% confidence interval: 12,084-16,018). This peak was followed by a progressive reduction, with a nadir of 11,060 (10,393-11,727) occurring at P18 (P < 0.05 as compared with P7). Thereafter, glomerular number remained constant. Complementary immunohistochemical examination of vascular regression markers showed peak expression of glomerular ANGPT2 and THBS1 at P14. CONCLUSION: Our study reveals that the tissue- and time-saving Weibel-Gomez method commonly used to assess glomerular number is valid only after P18. The data indicate that regulation of glomerular number by regression occurs in normally maturing mouse kidneys. These findings suggest that the process of glomerular regression could be therapeutically targeted to prevent oligonephronia, which otherwise predisposes to chronic kidney disease. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/22717689/Maturational_regression_of_glomeruli_determines_the_nephron_population_in_normal_mice L2 - http://dx.doi.org/10.1038/pr.2012.81 DB - PRIME DP - Unbound Medicine ER -