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Downregulated miR-195 detected in preeclamptic placenta affects trophoblast cell invasion via modulating ActRIIA expression.
PLoS One 2012; 7(6):e38875Plos

Abstract

BACKGROUND

Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown.

METHODOLOGY/PRINCIPAL FINDINGS

Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195 and mature miR-195 expressions were down-regulated, whereas ActRIIA level appeared to be increased when compared with that in gestational-week-matched normal placentas.

CONCLUSIONS/SIGNIFICANCE

This is the first report on the function of miR-195 in human placental trophoblast cells which reveals an invasion-promoting effect of the small RNA via repressing ActRIIA. Aberrant expression of miR-195 may contribute to the occurrence of preeclampsia through interfering with Activin/Nodal signaling mediated by ActRIIA in human placenta.

Authors+Show Affiliations

State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22723898

Citation

Bai, Yang, et al. "Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion Via Modulating ActRIIA Expression." PloS One, vol. 7, no. 6, 2012, pp. e38875.
Bai Y, Yang W, Yang HX, et al. Downregulated miR-195 detected in preeclamptic placenta affects trophoblast cell invasion via modulating ActRIIA expression. PLoS ONE. 2012;7(6):e38875.
Bai, Y., Yang, W., Yang, H. X., Liao, Q., Ye, G., Fu, G., ... Wang, Y. L. (2012). Downregulated miR-195 detected in preeclamptic placenta affects trophoblast cell invasion via modulating ActRIIA expression. PloS One, 7(6), pp. e38875. doi:10.1371/journal.pone.0038875.
Bai Y, et al. Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion Via Modulating ActRIIA Expression. PLoS ONE. 2012;7(6):e38875. PubMed PMID: 22723898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulated miR-195 detected in preeclamptic placenta affects trophoblast cell invasion via modulating ActRIIA expression. AU - Bai,Yang, AU - Yang,Weiwei, AU - Yang,Hui-xia, AU - Liao,Qinping, AU - Ye,Gang, AU - Fu,Guodong, AU - Ji,Lei, AU - Xu,Peng, AU - Wang,Hao, AU - Li,Yu-xia, AU - Peng,Chun, AU - Wang,Yan-ling, Y1 - 2012/06/19/ PY - 2012/01/28/received PY - 2012/05/14/accepted PY - 2012/6/23/entrez PY - 2012/6/23/pubmed PY - 2012/12/13/medline SP - e38875 EP - e38875 JF - PloS one JO - PLoS ONE VL - 7 IS - 6 N2 - BACKGROUND: Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195 and mature miR-195 expressions were down-regulated, whereas ActRIIA level appeared to be increased when compared with that in gestational-week-matched normal placentas. CONCLUSIONS/SIGNIFICANCE: This is the first report on the function of miR-195 in human placental trophoblast cells which reveals an invasion-promoting effect of the small RNA via repressing ActRIIA. Aberrant expression of miR-195 may contribute to the occurrence of preeclampsia through interfering with Activin/Nodal signaling mediated by ActRIIA in human placenta. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22723898/Downregulated_miR_195_detected_in_preeclamptic_placenta_affects_trophoblast_cell_invasion_via_modulating_ActRIIA_expression_ L2 - http://dx.plos.org/10.1371/journal.pone.0038875 DB - PRIME DP - Unbound Medicine ER -