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Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis.
Diagn Microbiol Infect Dis. 2012 Sep; 74(1):49-53.DM

Abstract

Trichomonas vaginalis accounts for nearly half of all curable sexually transmitted diseases worldwide with serious health consequences. Effort to increase the sensitivity of its diagnosis is critical to both control measures and epidemiologic studies. This study was conducted to evaluate the OSOM® Trichomonas Rapid Test (Sekisui Diagnostics, Framingham, MA, USA), a qualitative antigen-detection immunochromatographic (IC) assay in the diagnosis of vaginal trichomoniasis comparable to the conventional methods. The study enrolled 258 females aged 18-50 years classified into symptomatic (185) and asymptomatic (73) groups. Vaginal swab specimens were obtained for wet mount, stained preparation (Giemsa, acridine-orange), culture (InPouch TV™, modified Diamond's), and for rapid OSOM testing. Trichomonas vaginalis was detected in 67, 66, 71, 99, 96, and 97 using wet mount, acridine-orange stain, Giemsa stain, modified Diamond's, InPouch media, and OSOM test, respectively. In comparison to a composite reference standard (CRS) of wet mount microscopy and culture, OSOM test reported 97.98%, 99.37%, 98.98%, 98.75%, and 98.84% for sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy, respectively. The OSOM test proved to be a simple and objective test. This rapid point-of-care-test will contribute significantly in the diagnosis of vaginal trichomoniasis and will increase the understanding of its still vague epidemiology.

Authors+Show Affiliations

Department of Medical Parasitology, Faculty of Medicine, Mansoura University, Egypt.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

22727836

Citation

Hegazy, Mamdouh M., et al. "Performance of Rapid Immunochromatographic Assay in the Diagnosis of Trichomoniasis Vaginalis." Diagnostic Microbiology and Infectious Disease, vol. 74, no. 1, 2012, pp. 49-53.
Hegazy MM, El-Tantawy NL, Soliman MM, et al. Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis. Diagn Microbiol Infect Dis. 2012;74(1):49-53.
Hegazy, M. M., El-Tantawy, N. L., Soliman, M. M., El-Sadeek, E. S., & El-Nagar, H. S. (2012). Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis. Diagnostic Microbiology and Infectious Disease, 74(1), 49-53. https://doi.org/10.1016/j.diagmicrobio.2012.05.003
Hegazy MM, et al. Performance of Rapid Immunochromatographic Assay in the Diagnosis of Trichomoniasis Vaginalis. Diagn Microbiol Infect Dis. 2012;74(1):49-53. PubMed PMID: 22727836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis. AU - Hegazy,Mamdouh M, AU - El-Tantawy,Nora L, AU - Soliman,Mohamed M, AU - El-Sadeek,Ehaab S, AU - El-Nagar,Hassan S, Y1 - 2012/06/21/ PY - 2012/01/31/received PY - 2012/05/01/revised PY - 2012/05/07/accepted PY - 2012/6/26/entrez PY - 2012/6/26/pubmed PY - 2013/1/9/medline SP - 49 EP - 53 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 74 IS - 1 N2 - Trichomonas vaginalis accounts for nearly half of all curable sexually transmitted diseases worldwide with serious health consequences. Effort to increase the sensitivity of its diagnosis is critical to both control measures and epidemiologic studies. This study was conducted to evaluate the OSOM® Trichomonas Rapid Test (Sekisui Diagnostics, Framingham, MA, USA), a qualitative antigen-detection immunochromatographic (IC) assay in the diagnosis of vaginal trichomoniasis comparable to the conventional methods. The study enrolled 258 females aged 18-50 years classified into symptomatic (185) and asymptomatic (73) groups. Vaginal swab specimens were obtained for wet mount, stained preparation (Giemsa, acridine-orange), culture (InPouch TV™, modified Diamond's), and for rapid OSOM testing. Trichomonas vaginalis was detected in 67, 66, 71, 99, 96, and 97 using wet mount, acridine-orange stain, Giemsa stain, modified Diamond's, InPouch media, and OSOM test, respectively. In comparison to a composite reference standard (CRS) of wet mount microscopy and culture, OSOM test reported 97.98%, 99.37%, 98.98%, 98.75%, and 98.84% for sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy, respectively. The OSOM test proved to be a simple and objective test. This rapid point-of-care-test will contribute significantly in the diagnosis of vaginal trichomoniasis and will increase the understanding of its still vague epidemiology. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/22727836/Performance_of_rapid_immunochromatographic_assay_in_the_diagnosis_of_Trichomoniasis_vaginalis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(12)00170-8 DB - PRIME DP - Unbound Medicine ER -