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Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development.
Dev Biol. 2012 Sep 01; 369(1):133-49.DB

Abstract

Distinct groups of dopaminergic neurons develop at defined anatomical sites in the brain to modulate function of a large diversity of local and far-ranging circuits. However, the molecular identity as judged from transcription factor expression has not been determined for all dopaminergic groups. Here, we analyze regional expression of transcription factors in the larval zebrafish brain to determine co-expression with the Tyrosine hydroxylase marker in dopaminergic neurons. We define sets of transcription factors that clearly identify each dopaminergic group. These data confirm postulated relations to dopaminergic groups defined for mammalian systems. We focus our functional analysis on prethalamic dopaminergic neurons, which co-express the transcription factors Arx and Isl1. Morpholino-based knockdown reveals that both Arx and Isl1 are strictly required for prethalamic dopaminergic neuron development and appear to act in parallel. We further show that Arx contributes to patterning in the prethalamic region, while Isl1 is required for differentiation of prethalamic dopaminergic neurons.

Authors+Show Affiliations

Developmental Biology, Institute Biology 1, University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22728160

Citation

Filippi, Alida, et al. "Analysis of Transcriptional Codes for Zebrafish Dopaminergic Neurons Reveals Essential Functions of Arx and Isl1 in Prethalamic Dopaminergic Neuron Development." Developmental Biology, vol. 369, no. 1, 2012, pp. 133-49.
Filippi A, Jainok C, Driever W. Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development. Dev Biol. 2012;369(1):133-49.
Filippi, A., Jainok, C., & Driever, W. (2012). Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development. Developmental Biology, 369(1), 133-49. https://doi.org/10.1016/j.ydbio.2012.06.010
Filippi A, Jainok C, Driever W. Analysis of Transcriptional Codes for Zebrafish Dopaminergic Neurons Reveals Essential Functions of Arx and Isl1 in Prethalamic Dopaminergic Neuron Development. Dev Biol. 2012 Sep 1;369(1):133-49. PubMed PMID: 22728160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development. AU - Filippi,Alida, AU - Jainok,Chamaiphorn, AU - Driever,Wolfgang, Y1 - 2012/06/20/ PY - 2012/01/19/received PY - 2012/05/16/revised PY - 2012/06/12/accepted PY - 2012/6/26/entrez PY - 2012/6/26/pubmed PY - 2012/10/17/medline SP - 133 EP - 49 JF - Developmental biology JO - Dev. Biol. VL - 369 IS - 1 N2 - Distinct groups of dopaminergic neurons develop at defined anatomical sites in the brain to modulate function of a large diversity of local and far-ranging circuits. However, the molecular identity as judged from transcription factor expression has not been determined for all dopaminergic groups. Here, we analyze regional expression of transcription factors in the larval zebrafish brain to determine co-expression with the Tyrosine hydroxylase marker in dopaminergic neurons. We define sets of transcription factors that clearly identify each dopaminergic group. These data confirm postulated relations to dopaminergic groups defined for mammalian systems. We focus our functional analysis on prethalamic dopaminergic neurons, which co-express the transcription factors Arx and Isl1. Morpholino-based knockdown reveals that both Arx and Isl1 are strictly required for prethalamic dopaminergic neuron development and appear to act in parallel. We further show that Arx contributes to patterning in the prethalamic region, while Isl1 is required for differentiation of prethalamic dopaminergic neurons. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/22728160/Analysis_of_transcriptional_codes_for_zebrafish_dopaminergic_neurons_reveals_essential_functions_of_Arx_and_Isl1_in_prethalamic_dopaminergic_neuron_development_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(12)00319-3 DB - PRIME DP - Unbound Medicine ER -