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Pharmacological properties and protein binding capacity of phenolic extracts of some Venda medicinal plants used against cough and fever.
J Ethnopharmacol. 2012 Aug 30; 143(1):185-93.JE

Abstract

ETHNOPHARMACOLOGY RELEVANCE

Several ailments are caused by infectious bacteria and in other diseases; they act as co-infection which complicate human life by causing health hazards. In Venda (South Africa), many plants are used in traditional medicine to treat cough and fever.

AIM OF THE STUDY

This study was aimed at evaluating the antibacterial and antifungal properties, cyclooxygenases (COX), acetylcholinesterase (AChE) enzyme inhibitory effects and the phenolic composition as well as mutagenic properties of six medicinal plants used by the Venda people of Limpopo Province of South Africa against cough and fever.

MATERIALS AND METHODS

The petroleum ether (PE), dichloromethane (DCM), 80% ethanol (EtOH) and water extracts of six plants were tested against four infectious bacteria (Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus) and a fungus Candida albicans. The same extracts were evaluated for their ability to inhibit COX-1 and -2 enzymes. Methanolic and water extracts of the same plant were tested for acetylcholinesterase inhibitory effects. Total phenolics, flavonoids, gallotannins and condensed tannins were determined. The ability of the extracts to bind and precipitate proteins was also investigated. The extracts were investigated for genotoxicity with and without S9 (metabolic activation) against three Salmonella typhimurium tester strains TA98, TA100 and TA102.

RESULTS

The organic extracts of Rhus lancea leaves exhibited the best antibacterial activity with minimum inhibitory concentration (MIC) values ranging from 0.0061 to 0.049mg/ml. The best antifungal activity was observed from a DCM extract of Syzygium cordatum leaves with a MIC value of 0.195mg/ml. The methanolic and water extracts of the same plant exhibited high inhibitory effects towards AChE with IC(50) values of 0.22 and 0.26mg/ml, respectively. The highest levels of flavonoids and gallotannins were detected in Spirostachys africana bark; 11.57 and 48.88μg/g, respectively. The highest percentages (1.2%) of condensed tannins were detected in Uvaria caffra leaves. The high levels of phenolic compounds may have been responsible for high antimicrobial activities for extracts of S. africana bark and U. caffra leaves. S. cordatum leaves represented the highest affinity for protein binding with 93%. All the extracts were non-mutagenic towards the three tested strains with and without S9 metabolic activation.

CONCLUSION

The result obtained in this study goes a long way in validating the ethnobotanical usage of these medicinal plants in the treatment of cough and fever by the Venda people. However, more evidence obtainable from other assays not performed here are urgently required to confirm these results.

Authors+Show Affiliations

Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22732728

Citation

Mulaudzi, R B., et al. "Pharmacological Properties and Protein Binding Capacity of Phenolic Extracts of some Venda Medicinal Plants Used Against Cough and Fever." Journal of Ethnopharmacology, vol. 143, no. 1, 2012, pp. 185-93.
Mulaudzi RB, Ndhlala AR, Kulkarni MG, et al. Pharmacological properties and protein binding capacity of phenolic extracts of some Venda medicinal plants used against cough and fever. J Ethnopharmacol. 2012;143(1):185-93.
Mulaudzi, R. B., Ndhlala, A. R., Kulkarni, M. G., & Van Staden, J. (2012). Pharmacological properties and protein binding capacity of phenolic extracts of some Venda medicinal plants used against cough and fever. Journal of Ethnopharmacology, 143(1), 185-93. https://doi.org/10.1016/j.jep.2012.06.022
Mulaudzi RB, et al. Pharmacological Properties and Protein Binding Capacity of Phenolic Extracts of some Venda Medicinal Plants Used Against Cough and Fever. J Ethnopharmacol. 2012 Aug 30;143(1):185-93. PubMed PMID: 22732728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological properties and protein binding capacity of phenolic extracts of some Venda medicinal plants used against cough and fever. AU - Mulaudzi,R B, AU - Ndhlala,A R, AU - Kulkarni,M G, AU - Van Staden,J, Y1 - 2012/06/23/ PY - 2012/04/20/received PY - 2012/06/12/revised PY - 2012/06/12/accepted PY - 2012/6/27/entrez PY - 2012/6/27/pubmed PY - 2012/12/19/medline SP - 185 EP - 93 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 143 IS - 1 N2 - ETHNOPHARMACOLOGY RELEVANCE: Several ailments are caused by infectious bacteria and in other diseases; they act as co-infection which complicate human life by causing health hazards. In Venda (South Africa), many plants are used in traditional medicine to treat cough and fever. AIM OF THE STUDY: This study was aimed at evaluating the antibacterial and antifungal properties, cyclooxygenases (COX), acetylcholinesterase (AChE) enzyme inhibitory effects and the phenolic composition as well as mutagenic properties of six medicinal plants used by the Venda people of Limpopo Province of South Africa against cough and fever. MATERIALS AND METHODS: The petroleum ether (PE), dichloromethane (DCM), 80% ethanol (EtOH) and water extracts of six plants were tested against four infectious bacteria (Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus) and a fungus Candida albicans. The same extracts were evaluated for their ability to inhibit COX-1 and -2 enzymes. Methanolic and water extracts of the same plant were tested for acetylcholinesterase inhibitory effects. Total phenolics, flavonoids, gallotannins and condensed tannins were determined. The ability of the extracts to bind and precipitate proteins was also investigated. The extracts were investigated for genotoxicity with and without S9 (metabolic activation) against three Salmonella typhimurium tester strains TA98, TA100 and TA102. RESULTS: The organic extracts of Rhus lancea leaves exhibited the best antibacterial activity with minimum inhibitory concentration (MIC) values ranging from 0.0061 to 0.049mg/ml. The best antifungal activity was observed from a DCM extract of Syzygium cordatum leaves with a MIC value of 0.195mg/ml. The methanolic and water extracts of the same plant exhibited high inhibitory effects towards AChE with IC(50) values of 0.22 and 0.26mg/ml, respectively. The highest levels of flavonoids and gallotannins were detected in Spirostachys africana bark; 11.57 and 48.88μg/g, respectively. The highest percentages (1.2%) of condensed tannins were detected in Uvaria caffra leaves. The high levels of phenolic compounds may have been responsible for high antimicrobial activities for extracts of S. africana bark and U. caffra leaves. S. cordatum leaves represented the highest affinity for protein binding with 93%. All the extracts were non-mutagenic towards the three tested strains with and without S9 metabolic activation. CONCLUSION: The result obtained in this study goes a long way in validating the ethnobotanical usage of these medicinal plants in the treatment of cough and fever by the Venda people. However, more evidence obtainable from other assays not performed here are urgently required to confirm these results. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22732728/Pharmacological_properties_and_protein_binding_capacity_of_phenolic_extracts_of_some_Venda_medicinal_plants_used_against_cough_and_fever_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00421-7 DB - PRIME DP - Unbound Medicine ER -