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Sesquiterpene lactones and the diterpene 5-epi-icetexone affect the intracellular and extracellular stages of Trypanosoma cruzi.
Parasitol Int. 2012 Dec; 61(4):628-33.PI

Abstract

Chagas disease is a major health problem in Latin America and is caused by the parasitic protozoan Trypanosoma cruzi. Although many drugs have been used to alleviate the disease, these have been ineffective in the chronic phase and have also presented numerous side effects on patients. In this study we tested the effect of three sesquiterpene lactones (dehydroleucodine, helenalin and mexicanin) and a diterpene (5-epi-icetexone) on parasites (Y-strain) grown in host cells. At 48 h of treatment, the number of amastigotes inside the cells was lower than in the controls. This effect was observable at concentrations of 1.5-3.8 μM, which are of low cytotoxicity to host cells. In addition, the compounds caused a decrease in the percentage of infected cells. The treatments also reduced the presence of trypomastigotes in the extracellular medium. In all cases, helenalin was the most potent. The number of parasites per cell at 24h indicates the occurrence of multiple infection, which would also be affected by the compounds. However, we should not discard an effect on the proliferation and survival of parasites within the host cells. On the other hand, an additional effect on the differentiation of parasites and/or the survival of extracellular trypomastigotes might be possible. We conclude that these compounds are very effective against T. cruzi possibly by multiple mechanisms.

Authors+Show Affiliations

Laboratorio de Biología y Fisiología Celular, Dr. Francisco Bertini, Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad de Cuyo, Mendoza, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22735296

Citation

Lozano, Esteban, et al. "Sesquiterpene Lactones and the Diterpene 5-epi-icetexone Affect the Intracellular and Extracellular Stages of Trypanosoma Cruzi." Parasitology International, vol. 61, no. 4, 2012, pp. 628-33.
Lozano E, Barrera P, Salinas R, et al. Sesquiterpene lactones and the diterpene 5-epi-icetexone affect the intracellular and extracellular stages of Trypanosoma cruzi. Parasitol Int. 2012;61(4):628-33.
Lozano, E., Barrera, P., Salinas, R., Vega, I., Nieto, M., Tonn, C., Kemmerling, U., Mortara, R. A., & Sosa, M. A. (2012). Sesquiterpene lactones and the diterpene 5-epi-icetexone affect the intracellular and extracellular stages of Trypanosoma cruzi. Parasitology International, 61(4), 628-33. https://doi.org/10.1016/j.parint.2012.06.005
Lozano E, et al. Sesquiterpene Lactones and the Diterpene 5-epi-icetexone Affect the Intracellular and Extracellular Stages of Trypanosoma Cruzi. Parasitol Int. 2012;61(4):628-33. PubMed PMID: 22735296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sesquiterpene lactones and the diterpene 5-epi-icetexone affect the intracellular and extracellular stages of Trypanosoma cruzi. AU - Lozano,Esteban, AU - Barrera,Patricia, AU - Salinas,Romina, AU - Vega,Israel, AU - Nieto,Matías, AU - Tonn,Carlos, AU - Kemmerling,Ulrike, AU - Mortara,Renato A, AU - Sosa,Miguel A, Y1 - 2012/06/23/ PY - 2011/12/14/received PY - 2012/06/05/revised PY - 2012/06/18/accepted PY - 2012/6/28/entrez PY - 2012/6/28/pubmed PY - 2013/2/6/medline SP - 628 EP - 33 JF - Parasitology international JO - Parasitol Int VL - 61 IS - 4 N2 - Chagas disease is a major health problem in Latin America and is caused by the parasitic protozoan Trypanosoma cruzi. Although many drugs have been used to alleviate the disease, these have been ineffective in the chronic phase and have also presented numerous side effects on patients. In this study we tested the effect of three sesquiterpene lactones (dehydroleucodine, helenalin and mexicanin) and a diterpene (5-epi-icetexone) on parasites (Y-strain) grown in host cells. At 48 h of treatment, the number of amastigotes inside the cells was lower than in the controls. This effect was observable at concentrations of 1.5-3.8 μM, which are of low cytotoxicity to host cells. In addition, the compounds caused a decrease in the percentage of infected cells. The treatments also reduced the presence of trypomastigotes in the extracellular medium. In all cases, helenalin was the most potent. The number of parasites per cell at 24h indicates the occurrence of multiple infection, which would also be affected by the compounds. However, we should not discard an effect on the proliferation and survival of parasites within the host cells. On the other hand, an additional effect on the differentiation of parasites and/or the survival of extracellular trypomastigotes might be possible. We conclude that these compounds are very effective against T. cruzi possibly by multiple mechanisms. SN - 1873-0329 UR - https://www.unboundmedicine.com/medline/citation/22735296/Sesquiterpene_lactones_and_the_diterpene_5_epi_icetexone_affect_the_intracellular_and_extracellular_stages_of_Trypanosoma_cruzi_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5769(12)00090-6 DB - PRIME DP - Unbound Medicine ER -