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Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells.
J Ethnopharmacol. 2012 Jul 13; 142(2):337-45.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Eucommia ulmoides Oliv. Bark. (EUE), has commonly been used to fortify the muscles and lungs, lower blood pressure, prevent miscarriage, improve the tone of liver and kidneys, and promote longevity the traditional tonic medicines of Korea, China, and Japan.

AIM OF THE STUDY

In this study, we investigated that the neuroprotective activities and possible mechanisms of EUE aqueous extract in hydrogen peroxide (H(2)O(2))-induced neuronal cell death in human SH-SY5Y neuroblastoma cells.

MATERIAL AND METHOD

We examined the effects of EUE against H(2)O(2)-induced cytotoxicity, DNA condensation, the production of reactive oxygen species (ROS), loss of mitochondria membrane potential (MMP), the proteolysis of cleaved poly-ADP-ribose polymerase (PARP), and the expression of Bcl-2, Bcl-xL, cleaved caspase-3, and release of cytochrome c. Moreover, we attempted to determine whether EUE suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1/2 (ERK 1/2), and phosphoinositide 3-kinase (PI3K)/Akt.

RESULTS

Pretreatment with EUE increased cell viability and inhibited cytotoxicity and DNA condensation. EUE also attenuated the increase in ROS production and MMP reduction. Western blot data revealed that EUE inhibited H(2)O(2)-induced up- or down-regulation of cleaved PARP, cleaved caspase-3, Bcl-2, and Bcl-xL. The EUE inhibited release of cytochrome c from mitochondria to the cytosol, and significantly attenuated H(2)O(2)-induced phosphorylation of JNK, p38 MAPK, ERK 1/2, and PI3K/Akt.

CONCLUSION

The potent neuroprotective capacity of EUE, shown in these experiments, may potentially be applied in the prevention or treatment of neurodegenerative diseases such as Alzheimer's disease (AD).

Authors+Show Affiliations

Department of Pharmacology, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22735663

Citation

Kwon, Seung-Hwan, et al. "Eucommia Ulmoides Oliv. Bark. Protects Against Hydrogen Peroxide-induced Neuronal Cell Death in SH-SY5Y Cells." Journal of Ethnopharmacology, vol. 142, no. 2, 2012, pp. 337-45.
Kwon SH, Kim MJ, Ma SX, et al. Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells. J Ethnopharmacol. 2012;142(2):337-45.
Kwon, S. H., Kim, M. J., Ma, S. X., You, I. J., Hwang, J. Y., Oh, J. H., Kim, S. Y., Kim, H. C., Lee, S. Y., & Jang, C. G. (2012). Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells. Journal of Ethnopharmacology, 142(2), 337-45. https://doi.org/10.1016/j.jep.2012.04.010
Kwon SH, et al. Eucommia Ulmoides Oliv. Bark. Protects Against Hydrogen Peroxide-induced Neuronal Cell Death in SH-SY5Y Cells. J Ethnopharmacol. 2012 Jul 13;142(2):337-45. PubMed PMID: 22735663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells. AU - Kwon,Seung-Hwan, AU - Kim,Min-Jung, AU - Ma,Shi-Xun, AU - You,In-Jee, AU - Hwang,Ji-Young, AU - Oh,Ji-Hwan, AU - Kim,Sun-Yeou, AU - Kim,Hyoung-Chun, AU - Lee,Seok-Yong, AU - Jang,Choon-Gon, Y1 - 2012/05/14/ PY - 2011/10/10/received PY - 2012/03/20/revised PY - 2012/04/08/accepted PY - 2012/6/28/entrez PY - 2012/6/28/pubmed PY - 2012/10/24/medline SP - 337 EP - 45 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Eucommia ulmoides Oliv. Bark. (EUE), has commonly been used to fortify the muscles and lungs, lower blood pressure, prevent miscarriage, improve the tone of liver and kidneys, and promote longevity the traditional tonic medicines of Korea, China, and Japan. AIM OF THE STUDY: In this study, we investigated that the neuroprotective activities and possible mechanisms of EUE aqueous extract in hydrogen peroxide (H(2)O(2))-induced neuronal cell death in human SH-SY5Y neuroblastoma cells. MATERIAL AND METHOD: We examined the effects of EUE against H(2)O(2)-induced cytotoxicity, DNA condensation, the production of reactive oxygen species (ROS), loss of mitochondria membrane potential (MMP), the proteolysis of cleaved poly-ADP-ribose polymerase (PARP), and the expression of Bcl-2, Bcl-xL, cleaved caspase-3, and release of cytochrome c. Moreover, we attempted to determine whether EUE suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1/2 (ERK 1/2), and phosphoinositide 3-kinase (PI3K)/Akt. RESULTS: Pretreatment with EUE increased cell viability and inhibited cytotoxicity and DNA condensation. EUE also attenuated the increase in ROS production and MMP reduction. Western blot data revealed that EUE inhibited H(2)O(2)-induced up- or down-regulation of cleaved PARP, cleaved caspase-3, Bcl-2, and Bcl-xL. The EUE inhibited release of cytochrome c from mitochondria to the cytosol, and significantly attenuated H(2)O(2)-induced phosphorylation of JNK, p38 MAPK, ERK 1/2, and PI3K/Akt. CONCLUSION: The potent neuroprotective capacity of EUE, shown in these experiments, may potentially be applied in the prevention or treatment of neurodegenerative diseases such as Alzheimer's disease (AD). SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22735663/Eucommia_ulmoides_Oliv__Bark__protects_against_hydrogen_peroxide_induced_neuronal_cell_death_in_SH_SY5Y_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00237-1 DB - PRIME DP - Unbound Medicine ER -