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Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.
Appl Biochem Biotechnol. 2012 Sep; 168(2):234-46.AB

Abstract

The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.

Authors+Show Affiliations

Federal University of Sergipe, Av. Marechal Rondon s/n, 49100-000 Aracaju, SE, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22736274

Citation

Vilanova Neta, Jaci Lima, et al. "Bromelain Enzyme From Pineapple: in Vitro Activity Study Under Different Micropropagation Conditions." Applied Biochemistry and Biotechnology, vol. 168, no. 2, 2012, pp. 234-46.
Vilanova Neta JL, da Silva Lédo A, Lima AA, et al. Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions. Appl Biochem Biotechnol. 2012;168(2):234-46.
Vilanova Neta, J. L., da Silva Lédo, A., Lima, A. A., Santana, J. C., Leite, N. S., Ruzene, D. S., Silva, D. P., & de Souza, R. R. (2012). Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions. Applied Biochemistry and Biotechnology, 168(2), 234-46.
Vilanova Neta JL, et al. Bromelain Enzyme From Pineapple: in Vitro Activity Study Under Different Micropropagation Conditions. Appl Biochem Biotechnol. 2012;168(2):234-46. PubMed PMID: 22736274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions. AU - Vilanova Neta,Jaci Lima, AU - da Silva Lédo,Ana, AU - Lima,Aloisio André Bonfim, AU - Santana,José Carlos Curvelo, AU - Leite,Nadjma Souza, AU - Ruzene,Denise Santos, AU - Silva,Daniel Pereira, AU - de Souza,Roberto Rodrigues, Y1 - 2012/06/27/ PY - 2012/02/10/received PY - 2012/05/29/accepted PY - 2012/6/28/entrez PY - 2012/6/28/pubmed PY - 2013/2/12/medline SP - 234 EP - 46 JF - Applied biochemistry and biotechnology JO - Appl. Biochem. Biotechnol. VL - 168 IS - 2 N2 - The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/22736274/Bromelain_enzyme_from_pineapple:_in_vitro_activity_study_under_different_micropropagation_conditions_ L2 - https://dx.doi.org/10.1007/s12010-012-9753-1 DB - PRIME DP - Unbound Medicine ER -