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Distribution of glycinergic neurons in the brain of glycine transporter-2 transgenic Tg(glyt2:Gfp) adult zebrafish: relationship to brain-spinal descending systems.
J Comp Neurol. 2013 Feb 01; 521(2):389-425.JC

Abstract

We used a Tg(glyt2:gfp) transgenic zebrafish expressing the green fluorescent protein (GFP) under control of the glycine transporter 2 (GLYT2) regulatory sequences to study for the first time the glycinergic neurons in the brain of an adult teleost. We also performed in situ hybridization using a GLYT2 probe and glycine immunohistochemistry. This study was combined with biocytin tract tracing from the spinal cord to reveal descending glycinergic pathways. A few groups of GFP(+) /GLYT2(-) cells were observed in the midbrain and forebrain, including numerous pinealocytes. Conversely, a small nucleus of the midbrain tegmentum was GLYT2(+) but GFP(-) . Most of the GFP(+) and GLYT2(+) neurons were observed in the rhombencephalon and spinal cord, and a portion of these cells showed double GLYT2/GFP labeling. In the hindbrain, GFP/GLYT2(+) populations were observed in the medial octavolateral nucleus; the secondary, magnocellular, and descending octaval nuclei; the viscerosensory lobes; and reticular populations distributed from trigeminal to vagal levels. No glycinergic cells were observed in the cerebellum. Tract tracing revealed three conspicuous pairs of GFP/GLYT2(+) reticular neurons projecting to the spinal cord. In the spinal cord, GFP/GLYT2(+) cells were observed in the dorsal and ventral horns. GFP(+) fibers were observed from the olfactory bulbs to the spinal cord, although their density varied among regions. The Mauthner neurons received very rich GFP(+) innervation, mainly around the axon cap. Comparison of the zebrafish glycinergic system with the glycinergic systems of other adult vertebrates reveals shared patterns but also divergent traits in the evolution of this system.

Authors+Show Affiliations

Department of Cell Biology and Ecology, CIBUS, University of Santiago de Compostela, Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22736487

Citation

Barreiro-Iglesias, Antón, et al. "Distribution of Glycinergic Neurons in the Brain of Glycine Transporter-2 Transgenic Tg(glyt2:Gfp) Adult Zebrafish: Relationship to Brain-spinal Descending Systems." The Journal of Comparative Neurology, vol. 521, no. 2, 2013, pp. 389-425.
Barreiro-Iglesias A, Mysiak KS, Adrio F, et al. Distribution of glycinergic neurons in the brain of glycine transporter-2 transgenic Tg(glyt2:Gfp) adult zebrafish: relationship to brain-spinal descending systems. J Comp Neurol. 2013;521(2):389-425.
Barreiro-Iglesias, A., Mysiak, K. S., Adrio, F., Rodicio, M. C., Becker, C. G., Becker, T., & Anadón, R. (2013). Distribution of glycinergic neurons in the brain of glycine transporter-2 transgenic Tg(glyt2:Gfp) adult zebrafish: relationship to brain-spinal descending systems. The Journal of Comparative Neurology, 521(2), 389-425. https://doi.org/10.1002/cne.23179
Barreiro-Iglesias A, et al. Distribution of Glycinergic Neurons in the Brain of Glycine Transporter-2 Transgenic Tg(glyt2:Gfp) Adult Zebrafish: Relationship to Brain-spinal Descending Systems. J Comp Neurol. 2013 Feb 1;521(2):389-425. PubMed PMID: 22736487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of glycinergic neurons in the brain of glycine transporter-2 transgenic Tg(glyt2:Gfp) adult zebrafish: relationship to brain-spinal descending systems. AU - Barreiro-Iglesias,Antón, AU - Mysiak,Karolina Sandra, AU - Adrio,Fátima, AU - Rodicio,María Celina, AU - Becker,Catherina G, AU - Becker,Thomas, AU - Anadón,Ramón, PY - 2011/07/06/received PY - 2012/01/25/revised PY - 2012/06/21/accepted PY - 2012/6/28/entrez PY - 2012/6/28/pubmed PY - 2013/5/23/medline SP - 389 EP - 425 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 521 IS - 2 N2 - We used a Tg(glyt2:gfp) transgenic zebrafish expressing the green fluorescent protein (GFP) under control of the glycine transporter 2 (GLYT2) regulatory sequences to study for the first time the glycinergic neurons in the brain of an adult teleost. We also performed in situ hybridization using a GLYT2 probe and glycine immunohistochemistry. This study was combined with biocytin tract tracing from the spinal cord to reveal descending glycinergic pathways. A few groups of GFP(+) /GLYT2(-) cells were observed in the midbrain and forebrain, including numerous pinealocytes. Conversely, a small nucleus of the midbrain tegmentum was GLYT2(+) but GFP(-) . Most of the GFP(+) and GLYT2(+) neurons were observed in the rhombencephalon and spinal cord, and a portion of these cells showed double GLYT2/GFP labeling. In the hindbrain, GFP/GLYT2(+) populations were observed in the medial octavolateral nucleus; the secondary, magnocellular, and descending octaval nuclei; the viscerosensory lobes; and reticular populations distributed from trigeminal to vagal levels. No glycinergic cells were observed in the cerebellum. Tract tracing revealed three conspicuous pairs of GFP/GLYT2(+) reticular neurons projecting to the spinal cord. In the spinal cord, GFP/GLYT2(+) cells were observed in the dorsal and ventral horns. GFP(+) fibers were observed from the olfactory bulbs to the spinal cord, although their density varied among regions. The Mauthner neurons received very rich GFP(+) innervation, mainly around the axon cap. Comparison of the zebrafish glycinergic system with the glycinergic systems of other adult vertebrates reveals shared patterns but also divergent traits in the evolution of this system. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/22736487/Distribution_of_glycinergic_neurons_in_the_brain_of_glycine_transporter_2_transgenic_Tg_glyt2:Gfp__adult_zebrafish:_relationship_to_brain_spinal_descending_systems_ L2 - https://doi.org/10.1002/cne.23179 DB - PRIME DP - Unbound Medicine ER -