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Protective effects of Dunaliella salina - a carotenoids-rich alga - against ultraviolet B-induced corneal oxidative damage in mice.
Mol Vis. 2012; 18:1540-7.MV

Abstract

PURPOSE

Ultraviolet B (UVB) radiation from sunlight is known to be a risk factor for human corneal damage. The purpose of this study was to investigate the protective effects of Dunaliella salina (D. salina) on UVB radiation-induced corneal oxidative damage in male imprinting control region (ICR) mice.

METHODS

Corneal oxidative damage was induced by exposure to UVB radiation at 560 μW/cm(2). Animals were orally administered (gavage) D. salina at doses of 0, 123, and 615 mg/kg bodyweight/day for eight days. Corneal surface damages were graded according to smoothness and the extent of lissamine green staining. Corneal glutathione (GSH) and malondialdehyde (MDA) levels, as well as the activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), and glutathione reductase (GSH-Rd) in cornea were measured to monitor corneal injury.

RESULTS

UVB irradiation caused significant damage to the corneas, including apparent corneal ulcer and severe epithelial exfoliation, leading to decrease in the activities of SOD, catalase, GSH-Px, GSH-Rd, and GSH content in cornea, whereas there was increased corneal MDA content as compared with the control group. Treatment with D. salina could significantly (p<0.05) ameliorate corneal damage and increase the activities of SOD, catalase, GSH-Px, GSH-Rd, and GSH content, and decrease the MDA content in corneas when compared with the UVB-treated group.

CONCLUSIONS

The studies demonstrate that D. salina exhibits potent protective effects on UVB radiation-induced corneal oxidative damage in mice, likely due to both the increase of antioxidant enzyme activity and the inhibition of lipid peroxidation.

Authors+Show Affiliations

Department of Biotechnology, TransWorld University, Douliu City, Taiwan, ROC.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22736944

Citation

Tsai, Chia-Fang, et al. "Protective Effects of Dunaliella Salina - a Carotenoids-rich Alga - Against Ultraviolet B-induced Corneal Oxidative Damage in Mice." Molecular Vision, vol. 18, 2012, pp. 1540-7.
Tsai CF, Lu FJ, Hsu YW. Protective effects of Dunaliella salina - a carotenoids-rich alga - against ultraviolet B-induced corneal oxidative damage in mice. Mol Vis. 2012;18:1540-7.
Tsai, C. F., Lu, F. J., & Hsu, Y. W. (2012). Protective effects of Dunaliella salina - a carotenoids-rich alga - against ultraviolet B-induced corneal oxidative damage in mice. Molecular Vision, 18, 1540-7.
Tsai CF, Lu FJ, Hsu YW. Protective Effects of Dunaliella Salina - a Carotenoids-rich Alga - Against Ultraviolet B-induced Corneal Oxidative Damage in Mice. Mol Vis. 2012;18:1540-7. PubMed PMID: 22736944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of Dunaliella salina - a carotenoids-rich alga - against ultraviolet B-induced corneal oxidative damage in mice. AU - Tsai,Chia-Fang, AU - Lu,Fung-Jou, AU - Hsu,Yu-Wen, Y1 - 2012/06/13/ PY - 2012/02/16/received PY - 2012/06/10/accepted PY - 2012/6/28/entrez PY - 2012/6/28/pubmed PY - 2012/11/14/medline SP - 1540 EP - 7 JF - Molecular vision JO - Mol Vis VL - 18 N2 - PURPOSE: Ultraviolet B (UVB) radiation from sunlight is known to be a risk factor for human corneal damage. The purpose of this study was to investigate the protective effects of Dunaliella salina (D. salina) on UVB radiation-induced corneal oxidative damage in male imprinting control region (ICR) mice. METHODS: Corneal oxidative damage was induced by exposure to UVB radiation at 560 μW/cm(2). Animals were orally administered (gavage) D. salina at doses of 0, 123, and 615 mg/kg bodyweight/day for eight days. Corneal surface damages were graded according to smoothness and the extent of lissamine green staining. Corneal glutathione (GSH) and malondialdehyde (MDA) levels, as well as the activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), and glutathione reductase (GSH-Rd) in cornea were measured to monitor corneal injury. RESULTS: UVB irradiation caused significant damage to the corneas, including apparent corneal ulcer and severe epithelial exfoliation, leading to decrease in the activities of SOD, catalase, GSH-Px, GSH-Rd, and GSH content in cornea, whereas there was increased corneal MDA content as compared with the control group. Treatment with D. salina could significantly (p<0.05) ameliorate corneal damage and increase the activities of SOD, catalase, GSH-Px, GSH-Rd, and GSH content, and decrease the MDA content in corneas when compared with the UVB-treated group. CONCLUSIONS: The studies demonstrate that D. salina exhibits potent protective effects on UVB radiation-induced corneal oxidative damage in mice, likely due to both the increase of antioxidant enzyme activity and the inhibition of lipid peroxidation. SN - 1090-0535 UR - https://www.unboundmedicine.com/medline/citation/22736944/Protective_effects_of_Dunaliella_salina___a_carotenoids_rich_alga___against_ultraviolet_B_induced_corneal_oxidative_damage_in_mice_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22736944/ DB - PRIME DP - Unbound Medicine ER -