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The application of pseudo template molecularly imprinted polymer to the solid-phase extraction of cyromazine and its metabolic melamine from egg and milk.
J Sep Sci. 2012 Jun; 35(12):1432-8.JS

Abstract

The combination of molecularly imprinted polymer with high performance liquid chromatography has been developed to determine cyromazine and its metabolic melamine in some samples. However, the potential risk of template leakage used in molecularly imprinted polymer is a major disadvantage. To solve this problem, 2-(4,6-diamino-1,3,5-triazin-2-ylamino) ethanethiol disulfide, a molecule that shares the similar imprinting sites with cyromazine and melamine, was selected as pseudo template to prepare molecularly imprinted polymer. Methacrylic acid, ethylene glycol dimethyl acrylate and toluene were selected as functional monomer, crosslinker and porogen, respectively. The molecular recognition property and binding capability of cyromazine and melamine were evaluated by adsorption test and Scatchard analysis. The results showed that the molecularly imprinted polymer based on pseudo template had more excellent affinity and selectivity for cyromazine and melamine. The resulting molecularly imprinted polymer was used as a solid-phase extraction material to enrich cyromazine and melamine in egg and milk samples for high performance liquid chromatography analysis. The solid-phase extraction process was carefully optimized. It was found that when different concentration of cyromazine and melamine standards were spiked into samples, satisfactory recovery rate of cyromazine and melamine were obtained as 85.6-98.8% with relative standard deviation <5.5%.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Wang X
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
Fang Q
No affiliation info available
Liu S
No affiliation info available
Chen L
No affiliation info available

MeSH

AdsorptionAnimalsCattleChickensChromatography, High Pressure LiquidEggsFood ContaminationMilkMolecular ImprintingPolymersSolid Phase ExtractionTriazines

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22740253

Citation

Wang, Xianhua, et al. "The Application of Pseudo Template Molecularly Imprinted Polymer to the Solid-phase Extraction of Cyromazine and Its Metabolic Melamine From Egg and Milk." Journal of Separation Science, vol. 35, no. 12, 2012, pp. 1432-8.
Wang X, Fang Q, Liu S, et al. The application of pseudo template molecularly imprinted polymer to the solid-phase extraction of cyromazine and its metabolic melamine from egg and milk. J Sep Sci. 2012;35(12):1432-8.
Wang, X., Fang, Q., Liu, S., & Chen, L. (2012). The application of pseudo template molecularly imprinted polymer to the solid-phase extraction of cyromazine and its metabolic melamine from egg and milk. Journal of Separation Science, 35(12), 1432-8. https://doi.org/10.1002/jssc.201200096
Wang X, et al. The Application of Pseudo Template Molecularly Imprinted Polymer to the Solid-phase Extraction of Cyromazine and Its Metabolic Melamine From Egg and Milk. J Sep Sci. 2012;35(12):1432-8. PubMed PMID: 22740253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The application of pseudo template molecularly imprinted polymer to the solid-phase extraction of cyromazine and its metabolic melamine from egg and milk. AU - Wang,Xianhua, AU - Fang,Qiuxue, AU - Liu,Shipeng, AU - Chen,Lei, PY - 2012/6/29/entrez PY - 2012/6/29/pubmed PY - 2012/9/11/medline SP - 1432 EP - 8 JF - Journal of separation science JO - J Sep Sci VL - 35 IS - 12 N2 - The combination of molecularly imprinted polymer with high performance liquid chromatography has been developed to determine cyromazine and its metabolic melamine in some samples. However, the potential risk of template leakage used in molecularly imprinted polymer is a major disadvantage. To solve this problem, 2-(4,6-diamino-1,3,5-triazin-2-ylamino) ethanethiol disulfide, a molecule that shares the similar imprinting sites with cyromazine and melamine, was selected as pseudo template to prepare molecularly imprinted polymer. Methacrylic acid, ethylene glycol dimethyl acrylate and toluene were selected as functional monomer, crosslinker and porogen, respectively. The molecular recognition property and binding capability of cyromazine and melamine were evaluated by adsorption test and Scatchard analysis. The results showed that the molecularly imprinted polymer based on pseudo template had more excellent affinity and selectivity for cyromazine and melamine. The resulting molecularly imprinted polymer was used as a solid-phase extraction material to enrich cyromazine and melamine in egg and milk samples for high performance liquid chromatography analysis. The solid-phase extraction process was carefully optimized. It was found that when different concentration of cyromazine and melamine standards were spiked into samples, satisfactory recovery rate of cyromazine and melamine were obtained as 85.6-98.8% with relative standard deviation <5.5%. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/22740253/The_application_of_pseudo_template_molecularly_imprinted_polymer_to_the_solid_phase_extraction_of_cyromazine_and_its_metabolic_melamine_from_egg_and_milk_ L2 - https://doi.org/10.1002/jssc.201200096 DB - PRIME DP - Unbound Medicine ER -