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Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.).
Acta Sci Pol Technol Aliment. 2012 Jul-Sep; 11(3):293-301.AS

Abstract

BACKGROUND

Plant origin food ingredients are the main source of very potent antioxidants. Tocopherols, the main oilseeds natural antioxidants are very potent and when implemented into cell membranes are able to scavenge large number of free radicals. Among plant antioxidants are mainly phenolics, large and diversified group of chemical compounds with different radical scavenging potential.

MATERIAL AND METHODS

Defatted flaxseed meals were extracted with pure alcohols and its mixture with water. Acquired extracts were analysed for the content of phenolics and flavonoids using colorimetric procedures. Antioxidative capacity was assessed by utilizing: DPPH stable free radicals; inhibition of linoleic acid oxidation and reducing power of components.

RESULTS

Investigation was conducted on two different batches of flaxseed, assessing antioxidant capacity of compounds extracted with different polarity solvents and extracts were tested for antioxidant activity with different methods. The highest yield of extraction was achieved with 80% methanol but the extract did not contain the highest amount of phenolics and flavonoids. When 80% ethanol was used for extraction the highest amount of flavonoids was detected and also the best antioxidant capacity.

CONCLUSIONS

The results clearly showed that utilization of polar solvent enable extraction of significant amounts of phenolics and flavonoids. Those components were the most potent antioxidants present in those extracts. Content of these compounds correlated well with results from applied methods for antioxidant assessment.

Authors+Show Affiliations

Department of Chemistry, University of Sargodha, Sargodha 4010, Pakistan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22744950

Citation

Anwar, Farooq, and Roman Przybylski. "Effect of Solvents Extraction On Total Phenolics and Antioxidant Activity of Extracts From Flaxseed (Linum Usitatissimum L.)." Acta Scientiarum Polonorum. Technologia Alimentaria, vol. 11, no. 3, 2012, pp. 293-301.
Anwar F, Przybylski R. Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). Acta Sci Pol Technol Aliment. 2012;11(3):293-301.
Anwar, F., & Przybylski, R. (2012). Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). Acta Scientiarum Polonorum. Technologia Alimentaria, 11(3), 293-301.
Anwar F, Przybylski R. Effect of Solvents Extraction On Total Phenolics and Antioxidant Activity of Extracts From Flaxseed (Linum Usitatissimum L.). Acta Sci Pol Technol Aliment. 2012 Jul-Sep;11(3):293-301. PubMed PMID: 22744950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). AU - Anwar,Farooq, AU - Przybylski,Roman, PY - 2012/6/30/entrez PY - 2012/6/30/pubmed PY - 2012/10/27/medline SP - 293 EP - 301 JF - Acta scientiarum polonorum. Technologia alimentaria JO - Acta Sci Pol Technol Aliment VL - 11 IS - 3 N2 - BACKGROUND: Plant origin food ingredients are the main source of very potent antioxidants. Tocopherols, the main oilseeds natural antioxidants are very potent and when implemented into cell membranes are able to scavenge large number of free radicals. Among plant antioxidants are mainly phenolics, large and diversified group of chemical compounds with different radical scavenging potential. MATERIAL AND METHODS: Defatted flaxseed meals were extracted with pure alcohols and its mixture with water. Acquired extracts were analysed for the content of phenolics and flavonoids using colorimetric procedures. Antioxidative capacity was assessed by utilizing: DPPH stable free radicals; inhibition of linoleic acid oxidation and reducing power of components. RESULTS: Investigation was conducted on two different batches of flaxseed, assessing antioxidant capacity of compounds extracted with different polarity solvents and extracts were tested for antioxidant activity with different methods. The highest yield of extraction was achieved with 80% methanol but the extract did not contain the highest amount of phenolics and flavonoids. When 80% ethanol was used for extraction the highest amount of flavonoids was detected and also the best antioxidant capacity. CONCLUSIONS: The results clearly showed that utilization of polar solvent enable extraction of significant amounts of phenolics and flavonoids. Those components were the most potent antioxidants present in those extracts. Content of these compounds correlated well with results from applied methods for antioxidant assessment. SN - 1898-9594 UR - https://www.unboundmedicine.com/medline/citation/22744950/Effect_of_solvents_extraction_on_total_phenolics_and_antioxidant_activity_of_extracts_from_flaxseed__Linum_usitatissimum_L___ L2 - https://medlineplus.gov/antioxidants.html DB - PRIME DP - Unbound Medicine ER -