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Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans.
PLoS One. 2012; 7(6):e39515.Plos

Abstract

BACKGROUND

Monascus-fermented products are mentioned in an ancient Chinese pharmacopoeia of medicinal food and herbs. Monascus-fermented products offer valuable therapeutic benefits and have been extensively used in East Asia for several centuries. Several biological activities of Monascus-fermented products were recently described, and the extract of Monascus-fermented products showed strong antioxidant activity of scavenging DPPH radicals. To evaluate whether Monascus-fermented dioscorea products have potential as nutritional supplements, Monascus-fermented dioscorea's modulation of oxidative-stress resistance and associated regulatory mechanisms in Caenorhabditis elegans were investigated.

PRINCIPAL FINDINGS

We examined oxidative stress resistance of the ethanol extract of red mold dioscorea (RMDE) in C. elegans, and found that RMDE-treated wild-type C. elegans showed an increased survival during juglone-induced oxidative stress compared to untreated controls, whereas the antioxidant phenotype was absent from a daf-16 mutant. In addition, the RMDE reduced the level of intracellular reactive oxygen species in C. elegans. Finally, the RMDE affected the subcellular distribution of the FOXO transcription factor, DAF-16, in C. elegans and induced the expression of the sod-3 antioxidative gene.

CONCLUSIONS

These findings suggest that the RMDE acts as an antioxidative stress agent and thus may have potential as a nutritional supplement. Further studies in C. elegans suggest that the antioxidant effect of RMDE is mediated via regulation of the DAF-16/FOXO-dependent pathway.

Authors+Show Affiliations

Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22745774

Citation

Shi, Yeu-Ching, et al. "Monascus-fermented Dioscorea Enhances Oxidative Stress Resistance Via DAF-16/FOXO in Caenorhabditis Elegans." PloS One, vol. 7, no. 6, 2012, pp. e39515.
Shi YC, Yu CW, Liao VH, et al. Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PLoS ONE. 2012;7(6):e39515.
Shi, Y. C., Yu, C. W., Liao, V. H., & Pan, T. M. (2012). Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. PloS One, 7(6), e39515. https://doi.org/10.1371/journal.pone.0039515
Shi YC, et al. Monascus-fermented Dioscorea Enhances Oxidative Stress Resistance Via DAF-16/FOXO in Caenorhabditis Elegans. PLoS ONE. 2012;7(6):e39515. PubMed PMID: 22745774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans. AU - Shi,Yeu-Ching, AU - Yu,Chan-Wei, AU - Liao,Vivian Hsiu-Chuan, AU - Pan,Tzu-Ming, Y1 - 2012/06/22/ PY - 2011/12/31/received PY - 2012/05/27/accepted PY - 2012/6/30/entrez PY - 2012/6/30/pubmed PY - 2012/12/10/medline SP - e39515 EP - e39515 JF - PloS one JO - PLoS ONE VL - 7 IS - 6 N2 - BACKGROUND: Monascus-fermented products are mentioned in an ancient Chinese pharmacopoeia of medicinal food and herbs. Monascus-fermented products offer valuable therapeutic benefits and have been extensively used in East Asia for several centuries. Several biological activities of Monascus-fermented products were recently described, and the extract of Monascus-fermented products showed strong antioxidant activity of scavenging DPPH radicals. To evaluate whether Monascus-fermented dioscorea products have potential as nutritional supplements, Monascus-fermented dioscorea's modulation of oxidative-stress resistance and associated regulatory mechanisms in Caenorhabditis elegans were investigated. PRINCIPAL FINDINGS: We examined oxidative stress resistance of the ethanol extract of red mold dioscorea (RMDE) in C. elegans, and found that RMDE-treated wild-type C. elegans showed an increased survival during juglone-induced oxidative stress compared to untreated controls, whereas the antioxidant phenotype was absent from a daf-16 mutant. In addition, the RMDE reduced the level of intracellular reactive oxygen species in C. elegans. Finally, the RMDE affected the subcellular distribution of the FOXO transcription factor, DAF-16, in C. elegans and induced the expression of the sod-3 antioxidative gene. CONCLUSIONS: These findings suggest that the RMDE acts as an antioxidative stress agent and thus may have potential as a nutritional supplement. Further studies in C. elegans suggest that the antioxidant effect of RMDE is mediated via regulation of the DAF-16/FOXO-dependent pathway. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22745774/Monascus_fermented_dioscorea_enhances_oxidative_stress_resistance_via_DAF_16/FOXO_in_Caenorhabditis_elegans_ L2 - http://dx.plos.org/10.1371/journal.pone.0039515 DB - PRIME DP - Unbound Medicine ER -