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[Effect of ammonium ions on the uptake of L-leucine in Saccharomyces cerevisiae. Repression and inhibition of transport systems].
Rev Argent Microbiol. 1990 Jan-Mar; 22(1):7-16.RA

Abstract

L-leucine entrance into Saccharomyces cerevisiae is mediated by the general amino acid permease, GAP and two transport systems, S1 and S2, kinetically characterized. S1 is a high-affinity, low-velocity transport system, operating at lower L-leucine external concentration (0.05-0.1 mM), while S2 is a low-affinity, high-velocity transport system, operating at higher L-leucine external concentration (1.0 mM). In cells grown in minimal medium containing ammonium as sole nitrogen source the values of L-leucine entrance and uptake are smaller than those in cells grown in L-proline containing medium. When GAP is repressed by ammonium, L-leucine entrance is mediate by systems S1 and S2. Both systems are inhibited by ammonium. When GAP is derepressed, in cells grown in L-proline medium, L-leucine is transported by systems S1 and GAP (lower L-leucine external concentration), and mainly by S2 (higher L-leucine external concentration). GAP is the largest system inhibited by ammonium.

Authors+Show Affiliations

Kotliar N
Departamento de Bioquímica, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Stella CA
No affiliation info available
Ramos EH
No affiliation info available

MeSH

Amino Acid Transport SystemsAmmonium SulfateBiological TransportCitrullineFungal ProteinsKineticsLeucineMembrane Transport ModulatorsMembrane Transport ProteinsProlineSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

2274663

Citation

Kotliar, N, et al. "[Effect of Ammonium Ions On the Uptake of L-leucine in Saccharomyces Cerevisiae. Repression and Inhibition of Transport Systems]." Revista Argentina De Microbiologia, vol. 22, no. 1, 1990, pp. 7-16.
Kotliar N, Stella CA, Ramos EH. [Effect of ammonium ions on the uptake of L-leucine in Saccharomyces cerevisiae. Repression and inhibition of transport systems]. Rev Argent Microbiol. 1990;22(1):7-16.
Kotliar, N., Stella, C. A., & Ramos, E. H. (1990). [Effect of ammonium ions on the uptake of L-leucine in Saccharomyces cerevisiae. Repression and inhibition of transport systems]. Revista Argentina De Microbiologia, 22(1), 7-16.
Kotliar N, Stella CA, Ramos EH. [Effect of Ammonium Ions On the Uptake of L-leucine in Saccharomyces Cerevisiae. Repression and Inhibition of Transport Systems]. Rev Argent Microbiol. 1990 Jan-Mar;22(1):7-16. PubMed PMID: 2274663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of ammonium ions on the uptake of L-leucine in Saccharomyces cerevisiae. Repression and inhibition of transport systems]. AU - Kotliar,N, AU - Stella,C A, AU - Ramos,E H, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 7 EP - 16 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 22 IS - 1 N2 - L-leucine entrance into Saccharomyces cerevisiae is mediated by the general amino acid permease, GAP and two transport systems, S1 and S2, kinetically characterized. S1 is a high-affinity, low-velocity transport system, operating at lower L-leucine external concentration (0.05-0.1 mM), while S2 is a low-affinity, high-velocity transport system, operating at higher L-leucine external concentration (1.0 mM). In cells grown in minimal medium containing ammonium as sole nitrogen source the values of L-leucine entrance and uptake are smaller than those in cells grown in L-proline containing medium. When GAP is repressed by ammonium, L-leucine entrance is mediate by systems S1 and S2. Both systems are inhibited by ammonium. When GAP is derepressed, in cells grown in L-proline medium, L-leucine is transported by systems S1 and GAP (lower L-leucine external concentration), and mainly by S2 (higher L-leucine external concentration). GAP is the largest system inhibited by ammonium. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/2274663/[Effect_of_ammonium_ions_on_the_uptake_of_L_leucine_in_Saccharomyces_cerevisiae__Repression_and_inhibition_of_transport_systems]_ DB - PRIME DP - Unbound Medicine ER -