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Component-resolved diagnosis with commercially available D. pteronyssinus Der p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy.
Clin Exp Allergy. 2012 Sep; 42(9):1406-15).CE

Abstract

OBJECTIVE

To establish the prevalence and serum levels of IgE to commercial Der p 1, Der p 2, Der p 10 and the carbohydrate MUXF3 in house dust-mite allergic patients. To compare individual vs. allergen microarray methods.

METHODS

Prevalence and serum levels of IgE to Dermatophagoides pteronyssinus extract and components Der p 1, Der p 2, Der p 10 and MUXF3, specific IgG4 to D. pteronyssinus, total serum IgE levels, and clinical features (age, asthma, rhinitis and atopic dermatitis) were determined in 123 patients (64 children) with the ImmunoCAP® method. ImmunoCAP ISAC® was performed in 24 patients.

RESULTS

All patients had serum IgE to D. pteronyssinus. Prevalences of serum IgE to commercial components were Der p 1 93%, Der p 2 77% (Der p 1 or Der p 2 94%), Der p 10 28% and MUXF3 25%. Levels of D. pteronyssinus IgE strongly correlated with Der p 1 and Der p 2 IgE (r = 0.89 and 0.85 respectively), but not Der p 10 and MUXF3. ImmunoCAP® and ImmunoCAP ISAC® were concordant, but the quantitative correlation was poor. No clinical implication for the prevalence, levels, or molecular IgE reactivity profile to house dust mite components was found.

CONCLUSIONS AND CLINICAL RELEVANCE

Commercially available Der p 1 and Der p 2 strongly correlate with IgE D. pteronyssinus. The lack of Der p 1 and Der p 2 IgE may help with differential diagnosis. Der p 10 serum IgE prevalence and levels suggest different patterns in food and mite-related tropomyosin sensitization. Serum IgE to carbohydrate MUXF3, although unexpectedly prevalent, were low and did not modify D. pteronyssinus IgE levels. Follow-up may be best carried out with individual rather than microarrayed components.

Authors+Show Affiliations

Laboratoire d’Immunologie, Hôpital Conception 147 bd Baille F-13005 Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22747483

Citation

Bronnert, M, et al. "Component-resolved Diagnosis With Commercially Available D. Pteronyssinus Der P 1, Der P 2 and Der P 10: Relevant Markers for House Dust Mite Allergy." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 42, no. 9, 2012, 1406-15).
Bronnert M, Mancini J, Birnbaum J, et al. Component-resolved diagnosis with commercially available D. pteronyssinus Der p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy. Clin Exp Allergy. 2012;42(9):1406-15).
Bronnert, M., Mancini, J., Birnbaum, J., Agabriel, C., Liabeuf, V., Porri, F., Cleach, I., Fabre, A., Deneux, I., Grandné, V., Grob, J. J., Berbis, P., Charpin, D., Bongrand, P., & Vitte, J. (2012). Component-resolved diagnosis with commercially available D. pteronyssinus Der p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 42(9), 1406-15).
Bronnert M, et al. Component-resolved Diagnosis With Commercially Available D. Pteronyssinus Der P 1, Der P 2 and Der P 10: Relevant Markers for House Dust Mite Allergy. Clin Exp Allergy. 2012;42(9):1406-15). PubMed PMID: 22747483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Component-resolved diagnosis with commercially available D. pteronyssinus Der p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy. AU - Bronnert,M, AU - Mancini,J, AU - Birnbaum,J, AU - Agabriel,C, AU - Liabeuf,V, AU - Porri,F, AU - Cleach,I, AU - Fabre,A, AU - Deneux,I, AU - Grandné,V, AU - Grob,J-J, AU - Berbis,P, AU - Charpin,D, AU - Bongrand,P, AU - Vitte,J, PY - 2012/7/4/entrez PY - 2012/7/4/pubmed PY - 2013/1/11/medline SP - 1406 EP - 15) JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 42 IS - 9 N2 - OBJECTIVE: To establish the prevalence and serum levels of IgE to commercial Der p 1, Der p 2, Der p 10 and the carbohydrate MUXF3 in house dust-mite allergic patients. To compare individual vs. allergen microarray methods. METHODS: Prevalence and serum levels of IgE to Dermatophagoides pteronyssinus extract and components Der p 1, Der p 2, Der p 10 and MUXF3, specific IgG4 to D. pteronyssinus, total serum IgE levels, and clinical features (age, asthma, rhinitis and atopic dermatitis) were determined in 123 patients (64 children) with the ImmunoCAP® method. ImmunoCAP ISAC® was performed in 24 patients. RESULTS: All patients had serum IgE to D. pteronyssinus. Prevalences of serum IgE to commercial components were Der p 1 93%, Der p 2 77% (Der p 1 or Der p 2 94%), Der p 10 28% and MUXF3 25%. Levels of D. pteronyssinus IgE strongly correlated with Der p 1 and Der p 2 IgE (r = 0.89 and 0.85 respectively), but not Der p 10 and MUXF3. ImmunoCAP® and ImmunoCAP ISAC® were concordant, but the quantitative correlation was poor. No clinical implication for the prevalence, levels, or molecular IgE reactivity profile to house dust mite components was found. CONCLUSIONS AND CLINICAL RELEVANCE: Commercially available Der p 1 and Der p 2 strongly correlate with IgE D. pteronyssinus. The lack of Der p 1 and Der p 2 IgE may help with differential diagnosis. Der p 10 serum IgE prevalence and levels suggest different patterns in food and mite-related tropomyosin sensitization. Serum IgE to carbohydrate MUXF3, although unexpectedly prevalent, were low and did not modify D. pteronyssinus IgE levels. Follow-up may be best carried out with individual rather than microarrayed components. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/22747483/Component_resolved_diagnosis_with_commercially_available_D__pteronyssinus_Der_p_1_Der_p_2_and_Der_p_10:_relevant_markers_for_house_dust_mite_allergy_ L2 - https://doi.org/10.1111/j.1365-2222.2012.04035.x DB - PRIME DP - Unbound Medicine ER -