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Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak.
New Phytol. 2012 Sep; 195(4):883-93.NP

Abstract

Xanthomonas transcription activator-like (TAL) effectors promote disease in plants by binding to and activating host susceptibility genes. Plants counter with TAL effector-activated executor resistance genes, which cause host cell death and block disease progression. We asked whether the functional specificity of an executor gene could be broadened by adding different TAL effector binding elements (EBEs) to it. We added six EBEs to the rice Xa27 gene, which confers resistance to strains of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo) that deliver the TAL effector AvrXa27. The EBEs correspond to three other effectors from Xoo strain PXO99(A) and three from strain BLS256 of the bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola (Xoc). Stable integration into rice produced healthy lines exhibiting gene activation by each TAL effector, and resistance to PXO99(A) , a PXO99(A) derivative lacking AvrXa27, and BLS256, as well as two other Xoo and 10 Xoc strains virulent toward wildtype Xa27 plants. Transcripts initiated primarily at a common site. Sequences in the EBEs were found to occur nonrandomly in rice promoters, suggesting an overlap with endogenous regulatory sequences. Thus, executor gene specificity can be broadened by adding EBEs, but caution is warranted because of the possible coincident introduction of endogenous regulatory elements.

Authors+Show Affiliations

Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22747776

Citation

Hummel, Aaron W., et al. "Addition of Transcription Activator-like Effector Binding Sites to a Pathogen Strain-specific Rice Bacterial Blight Resistance Gene Makes It Effective Against Additional Strains and Against Bacterial Leaf Streak." The New Phytologist, vol. 195, no. 4, 2012, pp. 883-93.
Hummel AW, Doyle EL, Bogdanove AJ. Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak. New Phytol. 2012;195(4):883-93.
Hummel, A. W., Doyle, E. L., & Bogdanove, A. J. (2012). Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak. The New Phytologist, 195(4), 883-93. https://doi.org/10.1111/j.1469-8137.2012.04216.x
Hummel AW, Doyle EL, Bogdanove AJ. Addition of Transcription Activator-like Effector Binding Sites to a Pathogen Strain-specific Rice Bacterial Blight Resistance Gene Makes It Effective Against Additional Strains and Against Bacterial Leaf Streak. New Phytol. 2012;195(4):883-93. PubMed PMID: 22747776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak. AU - Hummel,Aaron W, AU - Doyle,Erin L, AU - Bogdanove,Adam J, Y1 - 2012/07/02/ PY - 2012/7/4/entrez PY - 2012/7/4/pubmed PY - 2012/12/10/medline SP - 883 EP - 93 JF - The New phytologist JO - New Phytol. VL - 195 IS - 4 N2 - Xanthomonas transcription activator-like (TAL) effectors promote disease in plants by binding to and activating host susceptibility genes. Plants counter with TAL effector-activated executor resistance genes, which cause host cell death and block disease progression. We asked whether the functional specificity of an executor gene could be broadened by adding different TAL effector binding elements (EBEs) to it. We added six EBEs to the rice Xa27 gene, which confers resistance to strains of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo) that deliver the TAL effector AvrXa27. The EBEs correspond to three other effectors from Xoo strain PXO99(A) and three from strain BLS256 of the bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola (Xoc). Stable integration into rice produced healthy lines exhibiting gene activation by each TAL effector, and resistance to PXO99(A) , a PXO99(A) derivative lacking AvrXa27, and BLS256, as well as two other Xoo and 10 Xoc strains virulent toward wildtype Xa27 plants. Transcripts initiated primarily at a common site. Sequences in the EBEs were found to occur nonrandomly in rice promoters, suggesting an overlap with endogenous regulatory sequences. Thus, executor gene specificity can be broadened by adding EBEs, but caution is warranted because of the possible coincident introduction of endogenous regulatory elements. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/22747776/Addition_of_transcription_activator_like_effector_binding_sites_to_a_pathogen_strain_specific_rice_bacterial_blight_resistance_gene_makes_it_effective_against_additional_strains_and_against_bacterial_leaf_streak_ L2 - https://doi.org/10.1111/j.1469-8137.2012.04216.x DB - PRIME DP - Unbound Medicine ER -