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Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes.
Proc Natl Acad Sci U S A. 2012 Jul 31; 109(31):E2101-9.PN

Abstract

Mammalian pigmentation is driven by the intercellular transfer of pigment-containing melanosomes from the tips of melanocyte dendrites to surrounding keratinocytes. Tip accumulation of melanosomes requires myosin Va, because melanosomes concentrate in the center of melanocytes from myosin Va-null (dilute) mice. This distribution defect results in inefficient melanosome transfer and a dilution of coat color. Dilute mice that simultaneously lack melanoregulin, the product of the dilute suppressor locus, exhibit a nearly complete restoration of coat color, but, surprisingly, melanosomes remain concentrated in the center of their melanocytes. Here we show that dilute/dsu melanocytes, but not dilute melanocytes, readily transfer the melanosomes concentrated in their center to surrounding keratinocytes in situ. Using time-lapse imaging of WT melanocyte/keratinocyte cocultures in which the plasma membranes of the two cells are marked with different colors, we define an intercellular melanosome transfer pathway that involves the shedding by the melanocyte of melanosome-rich packages, which subsequently are phagocytosed by the keratinocyte. Shedding, which occurs primarily at dendritic tips but also from more central regions, involves adhesion to the keratinocyte, thinning behind the forming package, and apparent self-abscission. Finally, we show that shedding from the cell center is sixfold more frequent in cultured dilute/dsu melanocytes than in dilute melanocytes, consistent with the in situ data. Together, these results explain how dsu restores the coat color of dilute mice without restoring intracellular melanosome distribution, indicate that melanoregulin is a negative regulator of melanosome transfer, and provide insight into the mechanism of intercellular melanosome transfer.

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Authors+Show Affiliations

Wu XS
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. wux@nhlbi.nih.gov
Masedunskas A
No affiliation info available
Weigert R
No affiliation info available
Copeland NG
No affiliation info available
Jenkins NA
No affiliation info available
Hammer JA
No affiliation info available

MeSH

Adaptor Proteins, Vesicular TransportAnimalsBiological Transport, ActiveCarrier ProteinsCells, CulturedCoculture TechniquesIntracellular Signaling Peptides and ProteinsKeratinocytesMelanocytesMelanosomesMiceMice, Mutant StrainsMyosin Heavy ChainsMyosin Type VSkin Pigmentation

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22753477

Citation

Wu, Xufeng S., et al. "Melanoregulin Regulates a Shedding Mechanism That Drives Melanosome Transfer From Melanocytes to Keratinocytes." Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 31, 2012, pp. E2101-9.
Wu XS, Masedunskas A, Weigert R, et al. Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes. Proc Natl Acad Sci U S A. 2012;109(31):E2101-9.
Wu, X. S., Masedunskas, A., Weigert, R., Copeland, N. G., Jenkins, N. A., & Hammer, J. A. (2012). Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes. Proceedings of the National Academy of Sciences of the United States of America, 109(31), E2101-9. https://doi.org/10.1073/pnas.1209397109
Wu XS, et al. Melanoregulin Regulates a Shedding Mechanism That Drives Melanosome Transfer From Melanocytes to Keratinocytes. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):E2101-9. PubMed PMID: 22753477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes. AU - Wu,Xufeng S, AU - Masedunskas,Andreas, AU - Weigert,Roberto, AU - Copeland,Neal G, AU - Jenkins,Nancy A, AU - Hammer,John A, Y1 - 2012/07/02/ PY - 2012/7/4/entrez PY - 2012/7/4/pubmed PY - 2012/10/27/medline SP - E2101 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 109 IS - 31 N2 - Mammalian pigmentation is driven by the intercellular transfer of pigment-containing melanosomes from the tips of melanocyte dendrites to surrounding keratinocytes. Tip accumulation of melanosomes requires myosin Va, because melanosomes concentrate in the center of melanocytes from myosin Va-null (dilute) mice. This distribution defect results in inefficient melanosome transfer and a dilution of coat color. Dilute mice that simultaneously lack melanoregulin, the product of the dilute suppressor locus, exhibit a nearly complete restoration of coat color, but, surprisingly, melanosomes remain concentrated in the center of their melanocytes. Here we show that dilute/dsu melanocytes, but not dilute melanocytes, readily transfer the melanosomes concentrated in their center to surrounding keratinocytes in situ. Using time-lapse imaging of WT melanocyte/keratinocyte cocultures in which the plasma membranes of the two cells are marked with different colors, we define an intercellular melanosome transfer pathway that involves the shedding by the melanocyte of melanosome-rich packages, which subsequently are phagocytosed by the keratinocyte. Shedding, which occurs primarily at dendritic tips but also from more central regions, involves adhesion to the keratinocyte, thinning behind the forming package, and apparent self-abscission. Finally, we show that shedding from the cell center is sixfold more frequent in cultured dilute/dsu melanocytes than in dilute melanocytes, consistent with the in situ data. Together, these results explain how dsu restores the coat color of dilute mice without restoring intracellular melanosome distribution, indicate that melanoregulin is a negative regulator of melanosome transfer, and provide insight into the mechanism of intercellular melanosome transfer. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/22753477/Melanoregulin_regulates_a_shedding_mechanism_that_drives_melanosome_transfer_from_melanocytes_to_keratinocytes_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=22753477 DB - PRIME DP - Unbound Medicine ER -