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Hedyotis diffusa Willd inhibits colorectal cancer growth in vivo via inhibition of STAT3 signaling pathway.
Int J Mol Sci 2012; 13(5):6117-28IJ

Abstract

Signal Transducer and Activator of Transcription 3 (STAT3), a common oncogenic mediator, is constitutively activated in many types of human cancers; therefore it is a major focus in the development of novel anti-cancer agents. Hedyotis diffusa Willd has been used as a major component in several Chinese medicine formulas for the clinical treatment of colorectal cancer (CRC). However, the precise mechanism of its anti-tumor activity remains largely unclear. Using a CRC mouse xenograft model, in the present study we evaluated the effect of the ethanol extract of Hedyotis diffusa Willd (EEHDW) on tumor growth in vivo and investigated the underlying molecular mechanisms. We found that EEHDW reduced tumor volume and tumor weight, but had no effect on body weight gain in CRC mice, demonstrating that EEHDW can inhibit CRC growth in vivo without apparent adverse effect. In addition, EEHDW treatment suppressed STAT3 phosphorylation in tumor tissues, which in turn resulted in the promotion of cancer cell apoptosis and inhibition of proliferation. Moreover, EEHDW treatment altered the expression pattern of several important target genes of the STAT3 signaling pathway, i.e., decreased expression of Cyclin D1, CDK4 and Bcl-2 as well as up-regulated p21 and Bax. These results suggest that suppression of the STAT3 pathway might be one of the mechanisms by which EEHDW treats colorectal cancer.

Authors+Show Affiliations

Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, 1 Huatuo Road, Minhou Shangjie, Fuzhou 350108, China; E-Mails: caiqiaoyan@fjtcm.edu.cn (Q.C.); linjiumao@gmail.com (J.L.); 282117657@qq.com (L.W.); 391118966@qq.com (L.Z.); 987131517@qq.com (L.W.); 691306289@qq.com (Y.Z.); zjwtcm@qq.com (J.Z.); 277708930@qq.com (A.S.); zfhong1953@163.com (Z.H.).No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22754353

Citation

Cai, Qiaoyan, et al. "Hedyotis Diffusa Willd Inhibits Colorectal Cancer Growth in Vivo Via Inhibition of STAT3 Signaling Pathway." International Journal of Molecular Sciences, vol. 13, no. 5, 2012, pp. 6117-28.
Cai Q, Lin J, Wei L, et al. Hedyotis diffusa Willd inhibits colorectal cancer growth in vivo via inhibition of STAT3 signaling pathway. Int J Mol Sci. 2012;13(5):6117-28.
Cai, Q., Lin, J., Wei, L., Zhang, L., Wang, L., Zhan, Y., ... Peng, J. (2012). Hedyotis diffusa Willd inhibits colorectal cancer growth in vivo via inhibition of STAT3 signaling pathway. International Journal of Molecular Sciences, 13(5), pp. 6117-28. doi:10.3390/ijms13056117.
Cai Q, et al. Hedyotis Diffusa Willd Inhibits Colorectal Cancer Growth in Vivo Via Inhibition of STAT3 Signaling Pathway. Int J Mol Sci. 2012;13(5):6117-28. PubMed PMID: 22754353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hedyotis diffusa Willd inhibits colorectal cancer growth in vivo via inhibition of STAT3 signaling pathway. AU - Cai,Qiaoyan, AU - Lin,Jiumao, AU - Wei,Lihui, AU - Zhang,Ling, AU - Wang,Lili, AU - Zhan,Youzhi, AU - Zeng,Jianwei, AU - Xu,Wei, AU - Shen,Aling, AU - Hong,Zhenfeng, AU - Peng,Jun, Y1 - 2012/05/18/ PY - 2012/03/28/received PY - 2012/05/03/revised PY - 2012/05/10/accepted PY - 2012/7/4/entrez PY - 2012/7/4/pubmed PY - 2012/7/4/medline KW - Chinese medicine KW - Hedyotis diffusa Willd KW - STAT3 pathway KW - apoptosis KW - colorectal cancer KW - proliferation SP - 6117 EP - 28 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 13 IS - 5 N2 - Signal Transducer and Activator of Transcription 3 (STAT3), a common oncogenic mediator, is constitutively activated in many types of human cancers; therefore it is a major focus in the development of novel anti-cancer agents. Hedyotis diffusa Willd has been used as a major component in several Chinese medicine formulas for the clinical treatment of colorectal cancer (CRC). However, the precise mechanism of its anti-tumor activity remains largely unclear. Using a CRC mouse xenograft model, in the present study we evaluated the effect of the ethanol extract of Hedyotis diffusa Willd (EEHDW) on tumor growth in vivo and investigated the underlying molecular mechanisms. We found that EEHDW reduced tumor volume and tumor weight, but had no effect on body weight gain in CRC mice, demonstrating that EEHDW can inhibit CRC growth in vivo without apparent adverse effect. In addition, EEHDW treatment suppressed STAT3 phosphorylation in tumor tissues, which in turn resulted in the promotion of cancer cell apoptosis and inhibition of proliferation. Moreover, EEHDW treatment altered the expression pattern of several important target genes of the STAT3 signaling pathway, i.e., decreased expression of Cyclin D1, CDK4 and Bcl-2 as well as up-regulated p21 and Bax. These results suggest that suppression of the STAT3 pathway might be one of the mechanisms by which EEHDW treats colorectal cancer. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/22754353/Hedyotis_diffusa_Willd_inhibits_colorectal_cancer_growth_in_vivo_via_inhibition_of_STAT3_signaling_pathway_ L2 - http://www.mdpi.com/resolver?pii=ijms-13-06117 DB - PRIME DP - Unbound Medicine ER -