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Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing.
Appl Microbiol Biotechnol. 2012 Sep; 95(5):1359-68.AM

Abstract

Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 °C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L(-1)) at 40 °C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L(-1), which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 °C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hu N
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, 266109, China.
Yuan B
No affiliation info available
Sun J
No affiliation info available
Wang SA
No affiliation info available
Li FL
No affiliation info available

MeSH

BiotechnologyDNA, FungalDNA, RibosomalEthanolFermentationGenes, rRNAGlycoside HydrolasesHelianthusInulinKluyveromycesMolecular Sequence DataRNA, FungalRNA, RibosomalSaccharomyces cerevisiaeSequence Analysis, DNATemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22760784

Citation

Hu, Nan, et al. "Thermotolerant Kluyveromyces Marxianus and Saccharomyces Cerevisiae Strains Representing Potentials for Bioethanol Production From Jerusalem Artichoke By Consolidated Bioprocessing." Applied Microbiology and Biotechnology, vol. 95, no. 5, 2012, pp. 1359-68.
Hu N, Yuan B, Sun J, et al. Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing. Appl Microbiol Biotechnol. 2012;95(5):1359-68.
Hu, N., Yuan, B., Sun, J., Wang, S. A., & Li, F. L. (2012). Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing. Applied Microbiology and Biotechnology, 95(5), 1359-68. https://doi.org/10.1007/s00253-012-4240-8
Hu N, et al. Thermotolerant Kluyveromyces Marxianus and Saccharomyces Cerevisiae Strains Representing Potentials for Bioethanol Production From Jerusalem Artichoke By Consolidated Bioprocessing. Appl Microbiol Biotechnol. 2012;95(5):1359-68. PubMed PMID: 22760784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing. AU - Hu,Nan, AU - Yuan,Bo, AU - Sun,Juan, AU - Wang,Shi-An, AU - Li,Fu-Li, Y1 - 2012/07/04/ PY - 2012/02/24/received PY - 2012/06/14/accepted PY - 2012/06/12/revised PY - 2012/7/5/entrez PY - 2012/7/5/pubmed PY - 2013/4/5/medline SP - 1359 EP - 68 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 95 IS - 5 N2 - Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 °C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L(-1)) at 40 °C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L(-1), which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 °C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/22760784/Thermotolerant_Kluyveromyces_marxianus_and_Saccharomyces_cerevisiae_strains_representing_potentials_for_bioethanol_production_from_Jerusalem_artichoke_by_consolidated_bioprocessing_ L2 - https://dx.doi.org/10.1007/s00253-012-4240-8 DB - PRIME DP - Unbound Medicine ER -