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ω6/ω3 Polyunsaturated fatty acid supplementations in renal cell model lead to a particular regulation through lipidome for preserved ω6/ω3 ratios.
Cell Mol Biol (Noisy-le-grand). 2012 Jun 30; 58 Suppl:OL1715-9.CM

Abstract

Polyunsaturated fatty acids (PUFA) supplementations modify cell lipid composition leading to a change in cell function. However, the effect of PUFA supplementations in renal model cell on the kidney epithelial cells membrane fatty acid profile is not known. Therefore, the purpose of this study was to determine the effects of PUFAs with different ω6/ω3 ratios supplementations in the kidney epithelial cells and the type of supplementation that can be used as cellular protection during kidney transplantation. For that, we used as model the LLCPK1 cell and determined their membrane fatty acid (FA) composition after supplementation with three different commercial food supplements. These supplements consist of S1: docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) with ω6/ω3 ratio = 0.1, S2: DHA, EPA, linoleic acid (LA) and γ-linoleic acid (GLA) with ω6/ω3 ratio = 2.5, or S3: α-linolenic acid (ALA) and LA with ω6/ω3 ratio near 1. Cells were incubated for 24 hr with 30 μM of ω3 fatty acids from each supplement. Fatty acid composition of control and experimental groups was analysed by gas chromatography after extraction of lipids and fatty acids methylation. The efficiency of cell PUFA supplementation was achieved by showing 2 to 4 fold increases in cell PUFA incorporation. Whatever the supplementation used, the cell saturated fatty acids (SFA) were decreased by 50% following the three supplementations used (p<0.001) as compared to control group. These decreases in SFA were compensated in part by increasing monounsaturated fatty acid levels. All these changes were observed with constant of cell ω6/ω3 ratio whatever the supplementations used. These data suggest that the supplements, with long chain polyunsaturated fatty acids or their precursors, lead to important regulation in the lipidome (desaturases and elongases) associated to preserved ω6/ω3 ratios. The fatty acids remodeling may represent an interesting new mechanism by which renal FA homoestasis could occurred.

Authors+Show Affiliations

INSERM U927, Faculté de Médecine, Université de Poitiers & CHU Miléterie, Poitiers, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22762527

Citation

Fares, M, et al. "Ω6/ω3 Polyunsaturated Fatty Acid Supplementations in Renal Cell Model Lead to a Particular Regulation Through Lipidome for Preserved Ω6/ω3 Ratios." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 58 Suppl, 2012, pp. OL1715-9.
Fares M, Armand M, Francois C, et al. Ω6/ω3 Polyunsaturated fatty acid supplementations in renal cell model lead to a particular regulation through lipidome for preserved ω6/ω3 ratios. Cell Mol Biol (Noisy-le-grand). 2012;58 Suppl:OL1715-9.
Fares, M., Armand, M., Francois, C., & Maixent, J. M. (2012). Ω6/ω3 Polyunsaturated fatty acid supplementations in renal cell model lead to a particular regulation through lipidome for preserved ω6/ω3 ratios. Cellular and Molecular Biology (Noisy-le-Grand, France), 58 Suppl, OL1715-9.
Fares M, et al. Ω6/ω3 Polyunsaturated Fatty Acid Supplementations in Renal Cell Model Lead to a Particular Regulation Through Lipidome for Preserved Ω6/ω3 Ratios. Cell Mol Biol (Noisy-le-grand). 2012 Jun 30;58 Suppl:OL1715-9. PubMed PMID: 22762527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ω6/ω3 Polyunsaturated fatty acid supplementations in renal cell model lead to a particular regulation through lipidome for preserved ω6/ω3 ratios. AU - Fares,M, AU - Armand,M, AU - Francois,C, AU - Maixent,J M, Y1 - 2012/06/30/ PY - 2012/03/20/received PY - 2012/06/13/accepted PY - 2012/7/6/entrez PY - 2012/7/13/pubmed PY - 2013/1/30/medline SP - OL1715 EP - 9 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 58 Suppl N2 - Polyunsaturated fatty acids (PUFA) supplementations modify cell lipid composition leading to a change in cell function. However, the effect of PUFA supplementations in renal model cell on the kidney epithelial cells membrane fatty acid profile is not known. Therefore, the purpose of this study was to determine the effects of PUFAs with different ω6/ω3 ratios supplementations in the kidney epithelial cells and the type of supplementation that can be used as cellular protection during kidney transplantation. For that, we used as model the LLCPK1 cell and determined their membrane fatty acid (FA) composition after supplementation with three different commercial food supplements. These supplements consist of S1: docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) with ω6/ω3 ratio = 0.1, S2: DHA, EPA, linoleic acid (LA) and γ-linoleic acid (GLA) with ω6/ω3 ratio = 2.5, or S3: α-linolenic acid (ALA) and LA with ω6/ω3 ratio near 1. Cells were incubated for 24 hr with 30 μM of ω3 fatty acids from each supplement. Fatty acid composition of control and experimental groups was analysed by gas chromatography after extraction of lipids and fatty acids methylation. The efficiency of cell PUFA supplementation was achieved by showing 2 to 4 fold increases in cell PUFA incorporation. Whatever the supplementation used, the cell saturated fatty acids (SFA) were decreased by 50% following the three supplementations used (p<0.001) as compared to control group. These decreases in SFA were compensated in part by increasing monounsaturated fatty acid levels. All these changes were observed with constant of cell ω6/ω3 ratio whatever the supplementations used. These data suggest that the supplements, with long chain polyunsaturated fatty acids or their precursors, lead to important regulation in the lipidome (desaturases and elongases) associated to preserved ω6/ω3 ratios. The fatty acids remodeling may represent an interesting new mechanism by which renal FA homoestasis could occurred. SN - 1165-158X UR - https://www.unboundmedicine.com/medline/citation/22762527/ω6/ω3_Polyunsaturated_fatty_acid_supplementations_in_renal_cell_model_lead_to_a_particular_regulation_through_lipidome_for_preserved_ω6/ω3_ratios_ L2 - https://antibodies.cancer.gov/detail/CPTC-FANCA-1 DB - PRIME DP - Unbound Medicine ER -