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Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate).
Bioprocess Biosyst Eng 2013; 36(2):185-92BB

Abstract

This paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. The main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. The analysis of the results was based on the response surface methodology and revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. The use of DOM yielded an 11.80-fold purification factor and 66.38 % biological activity recovery using poly(ethylene glycol) (PEG) with a molar mass of 4,000, 10.86 % PEG concentration (m/m) and 36.21 % saturation of ammonium sulphate.

Authors+Show Affiliations

Departamento de Engenharia de Sistemas Químicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas, Cidade Universitária "Zeferino Vaz", São Paulo, Brazil. dfcoelho@feq.unicamp.brNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22763776

Citation

Coelho, D F., et al. "Bromelain Purification Through Unconventional Aqueous Two-phase System (PEG/ammonium Sulphate)." Bioprocess and Biosystems Engineering, vol. 36, no. 2, 2013, pp. 185-92.
Coelho DF, Silveira E, Pessoa Junior A, et al. Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate). Bioprocess Biosyst Eng. 2013;36(2):185-92.
Coelho, D. F., Silveira, E., Pessoa Junior, A., & Tambourgi, E. B. (2013). Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate). Bioprocess and Biosystems Engineering, 36(2), pp. 185-92. doi:10.1007/s00449-012-0774-5.
Coelho DF, et al. Bromelain Purification Through Unconventional Aqueous Two-phase System (PEG/ammonium Sulphate). Bioprocess Biosyst Eng. 2013;36(2):185-92. PubMed PMID: 22763776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate). AU - Coelho,D F, AU - Silveira,E, AU - Pessoa Junior,A, AU - Tambourgi,E B, Y1 - 2012/07/05/ PY - 2011/12/05/received PY - 2012/06/12/accepted PY - 2012/7/6/entrez PY - 2012/7/6/pubmed PY - 2013/7/5/medline SP - 185 EP - 92 JF - Bioprocess and biosystems engineering JO - Bioprocess Biosyst Eng VL - 36 IS - 2 N2 - This paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. The main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. The analysis of the results was based on the response surface methodology and revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. The use of DOM yielded an 11.80-fold purification factor and 66.38 % biological activity recovery using poly(ethylene glycol) (PEG) with a molar mass of 4,000, 10.86 % PEG concentration (m/m) and 36.21 % saturation of ammonium sulphate. SN - 1615-7605 UR - https://www.unboundmedicine.com/medline/citation/22763776/Bromelain_purification_through_unconventional_aqueous_two_phase_system__PEG/ammonium_sulphate__ L2 - https://dx.doi.org/10.1007/s00449-012-0774-5 DB - PRIME DP - Unbound Medicine ER -