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Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in cocultures of dissociated DRG neurons and skeletal muscle cells in administration of NGF or NT-3.
Folia Histochem Cytobiol. 2012 Jul 05; 50(2):312-8.FH

Abstract

Both neurotrophins (NTs) and target skeletal muscle (SKM) cells are essential for the maintenance of the function of neurons and nerve-muscle communication. However, much less is known about the association of target SKM cells with distinct NTs on the expression of mRNAs for preprotachykinin (PPT), calcitonin-gene related peptide (CGRP), neurofilament 200 (NF-200), and microtubule associated protein 2 (MAP-2) in dorsal root ganglion (DRG) sensory neurons. In the present study, a neuromuscular coculture model of dissociated dorsal root ganglion (DRG) neurons and SKM cells was established. The morphology of DRG neurons and SKM cells in coculture was observed with an inverted phase contrast microscope. The effects of nerve growth factor (NGF) or neurotrophin-3 (NT-3) on the expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 was analyzed by real time-PCR assay. The morphology of DRG neuronal cell bodies and SKM cells in neuromuscular coculture at different conditions was similar. The neurons presented evidence of dense neurite outgrowth in the presence of distinct NTs in neuromuscular cocultures. NGF and NT-3 increased mRNA levels of PPT, CGRP, and NF-200, but not MAP-2, in neuromuscular cocultures. These results offer new clues towards a better understanding of the association of target SKM cells with distinct NTs on the expression of mRNAs for PPT, CGRP, NF-200 and MAP-2, and implicate the association of target SKM cells and NTs with DRG sensory neuronal phenotypes.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, Jinan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22763971

Citation

Zhang, Weiwei, et al. "Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in Cocultures of Dissociated DRG Neurons and Skeletal Muscle Cells in Administration of NGF or NT-3." Folia Histochemica Et Cytobiologica, vol. 50, no. 2, 2012, pp. 312-8.
Zhang W, Li H, Xing Z, et al. Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in cocultures of dissociated DRG neurons and skeletal muscle cells in administration of NGF or NT-3. Folia Histochem Cytobiol. 2012;50(2):312-8.
Zhang, W., Li, H., Xing, Z., Yuan, H., Kindy, M. S., & Li, Z. (2012). Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in cocultures of dissociated DRG neurons and skeletal muscle cells in administration of NGF or NT-3. Folia Histochemica Et Cytobiologica, 50(2), 312-8.
Zhang W, et al. Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in Cocultures of Dissociated DRG Neurons and Skeletal Muscle Cells in Administration of NGF or NT-3. Folia Histochem Cytobiol. 2012 Jul 5;50(2):312-8. PubMed PMID: 22763971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in cocultures of dissociated DRG neurons and skeletal muscle cells in administration of NGF or NT-3. AU - Zhang,Weiwei, AU - Li,Hao, AU - Xing,Ziying, AU - Yuan,Hongtu, AU - Kindy,Mark S, AU - Li,Zhenzhong, Y1 - 2012/07/05/ PY - 2012/02/03/received PY - 2012/02/03/accepted PY - 2012/7/6/entrez PY - 2012/7/6/pubmed PY - 2013/3/27/medline SP - 312 EP - 8 JF - Folia histochemica et cytobiologica JO - Folia Histochem. Cytobiol. VL - 50 IS - 2 N2 - Both neurotrophins (NTs) and target skeletal muscle (SKM) cells are essential for the maintenance of the function of neurons and nerve-muscle communication. However, much less is known about the association of target SKM cells with distinct NTs on the expression of mRNAs for preprotachykinin (PPT), calcitonin-gene related peptide (CGRP), neurofilament 200 (NF-200), and microtubule associated protein 2 (MAP-2) in dorsal root ganglion (DRG) sensory neurons. In the present study, a neuromuscular coculture model of dissociated dorsal root ganglion (DRG) neurons and SKM cells was established. The morphology of DRG neurons and SKM cells in coculture was observed with an inverted phase contrast microscope. The effects of nerve growth factor (NGF) or neurotrophin-3 (NT-3) on the expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 was analyzed by real time-PCR assay. The morphology of DRG neuronal cell bodies and SKM cells in neuromuscular coculture at different conditions was similar. The neurons presented evidence of dense neurite outgrowth in the presence of distinct NTs in neuromuscular cocultures. NGF and NT-3 increased mRNA levels of PPT, CGRP, and NF-200, but not MAP-2, in neuromuscular cocultures. These results offer new clues towards a better understanding of the association of target SKM cells with distinct NTs on the expression of mRNAs for PPT, CGRP, NF-200 and MAP-2, and implicate the association of target SKM cells and NTs with DRG sensory neuronal phenotypes. SN - 1897-5631 UR - https://www.unboundmedicine.com/medline/citation/22763971/Expression_of_mRNAs_for_PPT_CGRP_NF_200_and_MAP_2_in_cocultures_of_dissociated_DRG_neurons_and_skeletal_muscle_cells_in_administration_of_NGF_or_NT_3_ L2 - http://czasopisma.viamedica.pl/fhc/article/view/15786 DB - PRIME DP - Unbound Medicine ER -