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Phenotypes associated with down-regulation of Sl-IAA27 support functional diversity among Aux/IAA family members in tomato.
Plant Cell Physiol. 2012 Sep; 53(9):1583-95.PC

Abstract

The phytohormone auxin is known to regulate several aspects of plant development, and Aux/IAA transcription factors play a pivotal role in auxin signaling. To extend our understanding of the multiple functions of Aux/IAAs further, the present study describes the functional characterization of Sl-IAA27, a member of the tomato Aux/IAA gene family. Sl-IAA27 displays a distinct behavior compared with most Aux/IAA genes regarding the regulation of its expression by auxin, and the Sl-IAA27-encoded protein harbors a unique motif of unknown function also present in Sl-IAA9 and remarkably conserved in monocot and dicot species. Tomato transgenic plants underexpressing the Sl-IAA27 gene revealed multiple phenotypes related to vegetative and reproductive growth. Silencing of Sl-IAA27 results in higher auxin sensitivity, altered root development and reduced Chl content in leaves. Both ovule and pollen display a dramatic loss of fertility in Sl-IAA27 down-regulated lines, and the internal anatomy of the flower and the fruit are modified, with an enlarged placenta in smaller fruits. In line with the reduced Chl content in Sl-IAA27 RNA interference (RNAi) leaves, genes involved in Chl synthesis display lower expression at the level of transcript accumulation. Even though Sl-IAA27 is closely related to Sl-IAA9 in terms of sequence homology and the encoded proteins share common structural features, the data indicate that the two genes regulate tomato fruit initiation and development in a distinct manner.

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Authors+Show Affiliations

Bassa C
Université de Toulouse, INP-ENSA Toulouse, Génomique et Biotechnologie des Fruits, Avenue de l'Agrobiopole BP 32607, Castanet-Tolosan F-31326, France.
Mila I
No affiliation info available
Bouzayen M
No affiliation info available
Audran-Delalande C
No affiliation info available

MeSH

Amino Acid SequenceChlorophyllDown-RegulationFertilizationFlowersFruitGene Expression Regulation, PlantGenes, PlantIndoleacetic AcidsLycopersicon esculentumMolecular Sequence DataPhenotypePlant ProteinsPlant RootsPlants, Genetically ModifiedPollinationRNA InterferenceSeedsSubcellular FractionsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22764281

Citation

Bassa, Carole, et al. "Phenotypes Associated With Down-regulation of Sl-IAA27 Support Functional Diversity Among Aux/IAA Family Members in Tomato." Plant & Cell Physiology, vol. 53, no. 9, 2012, pp. 1583-95.
Bassa C, Mila I, Bouzayen M, et al. Phenotypes associated with down-regulation of Sl-IAA27 support functional diversity among Aux/IAA family members in tomato. Plant Cell Physiol. 2012;53(9):1583-95.
Bassa, C., Mila, I., Bouzayen, M., & Audran-Delalande, C. (2012). Phenotypes associated with down-regulation of Sl-IAA27 support functional diversity among Aux/IAA family members in tomato. Plant & Cell Physiology, 53(9), 1583-95. https://doi.org/10.1093/pcp/pcs101
Bassa C, et al. Phenotypes Associated With Down-regulation of Sl-IAA27 Support Functional Diversity Among Aux/IAA Family Members in Tomato. Plant Cell Physiol. 2012;53(9):1583-95. PubMed PMID: 22764281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypes associated with down-regulation of Sl-IAA27 support functional diversity among Aux/IAA family members in tomato. AU - Bassa,Carole, AU - Mila,Isabelle, AU - Bouzayen,Mondher, AU - Audran-Delalande,Corinne, Y1 - 2012/07/03/ PY - 2012/7/6/entrez PY - 2012/7/6/pubmed PY - 2013/7/3/medline SP - 1583 EP - 95 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 53 IS - 9 N2 - The phytohormone auxin is known to regulate several aspects of plant development, and Aux/IAA transcription factors play a pivotal role in auxin signaling. To extend our understanding of the multiple functions of Aux/IAAs further, the present study describes the functional characterization of Sl-IAA27, a member of the tomato Aux/IAA gene family. Sl-IAA27 displays a distinct behavior compared with most Aux/IAA genes regarding the regulation of its expression by auxin, and the Sl-IAA27-encoded protein harbors a unique motif of unknown function also present in Sl-IAA9 and remarkably conserved in monocot and dicot species. Tomato transgenic plants underexpressing the Sl-IAA27 gene revealed multiple phenotypes related to vegetative and reproductive growth. Silencing of Sl-IAA27 results in higher auxin sensitivity, altered root development and reduced Chl content in leaves. Both ovule and pollen display a dramatic loss of fertility in Sl-IAA27 down-regulated lines, and the internal anatomy of the flower and the fruit are modified, with an enlarged placenta in smaller fruits. In line with the reduced Chl content in Sl-IAA27 RNA interference (RNAi) leaves, genes involved in Chl synthesis display lower expression at the level of transcript accumulation. Even though Sl-IAA27 is closely related to Sl-IAA9 in terms of sequence homology and the encoded proteins share common structural features, the data indicate that the two genes regulate tomato fruit initiation and development in a distinct manner. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/22764281/Phenotypes_associated_with_down_regulation_of_Sl_IAA27_support_functional_diversity_among_Aux/IAA_family_members_in_tomato_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcs101 DB - PRIME DP - Unbound Medicine ER -