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[Effects of electroacupuncture on T-lymphocytes, spleen index, thymus index and lymphopoiesis levels in strenuous exercise-induced stress rats].
Zhen Ci Yan Jiu. 2012 Apr; 37(2):136-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on levels of T-lymphocytes, spleen index, thymus index and lymphopoiesis in strenuous exercise-induced immunosuppression rats, so as to study its mechanisms underlying improving immune function of strenuous training athletes.

METHODS

A total of 43 SD rats were randomly allocated to control (n = 10), model (n = 17) and EA (n = 16) groups. Immunosuppression model was established by forcing the rat to swim for 150 min in a water tank, once daily, 6 times a week and for 8 weeks. EA (2 Hz, < 1 mA) was applied to bilateral "Zusanli" (ST 36), "Baihui" (GV 20) and "Guanyuan" (CV 4) for 20 min, once daily for 7 weeks. Spleen weight and thymus weight were detected after killing the anesthetized rats for calculating spleen index and thymus index, separately. Peripheral blood T cell subsets were measured by using flow cytometer, and lymphopoiesis was detected by using 3H-thymidine deoxyribonucleotide (3H-TdR) incorporation.

RESULTS

In comparison with the control group, spleen index, thymus index, lymphopoiesis and CD3+ levels of the model group were decreased significantly (P < 0.01, P < 0.05). Compared with the model group, peripheral blood CD3+, CD4+ and NKC levels, and thymus and spleen indexes were increased considerably in EA group (P < 0.01, P < 0.05). No significant differences were found between the control and model groups in CD4+, CD8+ and CD4+ /CD8+ and NKC levels, and between the model and EA groups in CD8+ and CD4+/CD8+ levels (P > 0.05).

CONCLUSION

EA intervention can effectively upregulate peripheral blood CD3+, CD4+ and NKC, and thymus and spleen indexes in strenuous training rats, which may contribute to its effect in relieving strenuous exercise-induced immunosuppression.

Authors+Show Affiliations

College of Nursing, Guangzhou University of Chinese Medicine, Guangzhoug 510405, China. Luymwang@163.comNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22764600

Citation

Lu, Yong-Mei, and Hong Zhang. "[Effects of Electroacupuncture On T-lymphocytes, Spleen Index, Thymus Index and Lymphopoiesis Levels in Strenuous Exercise-induced Stress Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 2, 2012, pp. 136-9.
Lu YM, Zhang H. [Effects of electroacupuncture on T-lymphocytes, spleen index, thymus index and lymphopoiesis levels in strenuous exercise-induced stress rats]. Zhen Ci Yan Jiu. 2012;37(2):136-9.
Lu, Y. M., & Zhang, H. (2012). [Effects of electroacupuncture on T-lymphocytes, spleen index, thymus index and lymphopoiesis levels in strenuous exercise-induced stress rats]. Zhen Ci Yan Jiu = Acupuncture Research, 37(2), 136-9.
Lu YM, Zhang H. [Effects of Electroacupuncture On T-lymphocytes, Spleen Index, Thymus Index and Lymphopoiesis Levels in Strenuous Exercise-induced Stress Rats]. Zhen Ci Yan Jiu. 2012;37(2):136-9. PubMed PMID: 22764600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on T-lymphocytes, spleen index, thymus index and lymphopoiesis levels in strenuous exercise-induced stress rats]. AU - Lu,Yong-Mei, AU - Zhang,Hong, PY - 2012/7/7/entrez PY - 2012/7/7/pubmed PY - 2012/8/29/medline SP - 136 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 2 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on levels of T-lymphocytes, spleen index, thymus index and lymphopoiesis in strenuous exercise-induced immunosuppression rats, so as to study its mechanisms underlying improving immune function of strenuous training athletes. METHODS: A total of 43 SD rats were randomly allocated to control (n = 10), model (n = 17) and EA (n = 16) groups. Immunosuppression model was established by forcing the rat to swim for 150 min in a water tank, once daily, 6 times a week and for 8 weeks. EA (2 Hz, < 1 mA) was applied to bilateral "Zusanli" (ST 36), "Baihui" (GV 20) and "Guanyuan" (CV 4) for 20 min, once daily for 7 weeks. Spleen weight and thymus weight were detected after killing the anesthetized rats for calculating spleen index and thymus index, separately. Peripheral blood T cell subsets were measured by using flow cytometer, and lymphopoiesis was detected by using 3H-thymidine deoxyribonucleotide (3H-TdR) incorporation. RESULTS: In comparison with the control group, spleen index, thymus index, lymphopoiesis and CD3+ levels of the model group were decreased significantly (P < 0.01, P < 0.05). Compared with the model group, peripheral blood CD3+, CD4+ and NKC levels, and thymus and spleen indexes were increased considerably in EA group (P < 0.01, P < 0.05). No significant differences were found between the control and model groups in CD4+, CD8+ and CD4+ /CD8+ and NKC levels, and between the model and EA groups in CD8+ and CD4+/CD8+ levels (P > 0.05). CONCLUSION: EA intervention can effectively upregulate peripheral blood CD3+, CD4+ and NKC, and thymus and spleen indexes in strenuous training rats, which may contribute to its effect in relieving strenuous exercise-induced immunosuppression. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/22764600/[Effects_of_electroacupuncture_on_T_lymphocytes_spleen_index_thymus_index_and_lymphopoiesis_levels_in_strenuous_exercise_induced_stress_rats]_ DB - PRIME DP - Unbound Medicine ER -