Tags

Type your tag names separated by a space and hit enter

A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore development.
PLoS One. 2012; 7(6):e40246.Plos

Abstract

PsSAK1, a mitogen-activated protein (MAP) kinase from Phytophthora sojae, plays an important role in host infection and zoospore viability. However, the downstream mechanism of PsSAK1 remains unclear. In this study, the 3'-tag digital gene expression (DGE) profiling method was applied to sequence the global transcriptional sequence of PsSAK1-silenced mutants during the cysts stage and 1.5 h after inoculation onto susceptible soybean leaf tissues. Compared with the gene expression levels of the recipient P. sojae strain, several candidates of Myb family were differentially expressed (up or down) in response to the loss of PsSAK1, including of a R2R3-type Myb transcription factor, PsMYB1. qRT-PCR indicated that the transcriptional level of PsMYB1 decreased due to PsSAK1 silencing. The transcriptional level of PsMYB1 increased during sporulating hyphae, in germinated cysts, and early infection. Silencing of PsMYB1 results in three phenotypes: a) no cleavage of the cytoplasm into uninucleate zoospores or release of normal zoospores, b) direct germination of sporangia, and c) afunction in zoospore-mediated plant infection. Our data indicate that the PsMYB1 transcription factor functions downstream of MAP kinase PsSAK1 and is required for zoospore development of P. sojae.

Authors+Show Affiliations

Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22768262

Citation

Zhang, Meng, et al. "A Myb Transcription Factor of Phytophthora Sojae, Regulated By MAP Kinase PsSAK1, Is Required for Zoospore Development." PloS One, vol. 7, no. 6, 2012, pp. e40246.
Zhang M, Lu J, Tao K, et al. A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore development. PLoS ONE. 2012;7(6):e40246.
Zhang, M., Lu, J., Tao, K., Ye, W., Li, A., Liu, X., Kong, L., Dong, S., Zheng, X., & Wang, Y. (2012). A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore development. PloS One, 7(6), e40246. https://doi.org/10.1371/journal.pone.0040246
Zhang M, et al. A Myb Transcription Factor of Phytophthora Sojae, Regulated By MAP Kinase PsSAK1, Is Required for Zoospore Development. PLoS ONE. 2012;7(6):e40246. PubMed PMID: 22768262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore development. AU - Zhang,Meng, AU - Lu,Jing, AU - Tao,Kai, AU - Ye,Wenwu, AU - Li,Aining, AU - Liu,Xiaoyun, AU - Kong,Liang, AU - Dong,Suomeng, AU - Zheng,Xiaobo, AU - Wang,Yuanchao, Y1 - 2012/06/29/ PY - 2012/04/10/received PY - 2012/06/03/accepted PY - 2012/7/7/entrez PY - 2012/7/7/pubmed PY - 2012/12/10/medline SP - e40246 EP - e40246 JF - PloS one JO - PLoS ONE VL - 7 IS - 6 N2 - PsSAK1, a mitogen-activated protein (MAP) kinase from Phytophthora sojae, plays an important role in host infection and zoospore viability. However, the downstream mechanism of PsSAK1 remains unclear. In this study, the 3'-tag digital gene expression (DGE) profiling method was applied to sequence the global transcriptional sequence of PsSAK1-silenced mutants during the cysts stage and 1.5 h after inoculation onto susceptible soybean leaf tissues. Compared with the gene expression levels of the recipient P. sojae strain, several candidates of Myb family were differentially expressed (up or down) in response to the loss of PsSAK1, including of a R2R3-type Myb transcription factor, PsMYB1. qRT-PCR indicated that the transcriptional level of PsMYB1 decreased due to PsSAK1 silencing. The transcriptional level of PsMYB1 increased during sporulating hyphae, in germinated cysts, and early infection. Silencing of PsMYB1 results in three phenotypes: a) no cleavage of the cytoplasm into uninucleate zoospores or release of normal zoospores, b) direct germination of sporangia, and c) afunction in zoospore-mediated plant infection. Our data indicate that the PsMYB1 transcription factor functions downstream of MAP kinase PsSAK1 and is required for zoospore development of P. sojae. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22768262/A_Myb_transcription_factor_of_Phytophthora_sojae_regulated_by_MAP_kinase_PsSAK1_is_required_for_zoospore_development_ L2 - http://dx.plos.org/10.1371/journal.pone.0040246 DB - PRIME DP - Unbound Medicine ER -