Tags

Type your tag names separated by a space and hit enter

Effect of semax and its C-terminal fragment Pro-Gly-Pro on the expression of VEGF family genes and their receptors in experimental focal ischemia of the rat brain.
J Mol Neurosci. 2013 Feb; 49(2):328-33.JM

Abstract

The synthetic peptide Semax (Met-Glu-His-Phe-Pro-Gly-Pro) is used successfully in acute stroke therapy. In spite of numerous studies on the subject, many aspects of the neuroprotective effects of the peptide remain unknown. We studied the action of Semax and its C-terminal tripeptide Pro-Gly-Pro on the expression of the VEGF gene family (Vegf-a, Vegf-b, Vegf-c, Vegf-d, and Plgf) and their receptors (Vegfr-1, Vegfr-2, and Vegfr-3) in the frontoparietal cortex region of the rat brain at 3, 24, and 72 h after permanent left middle cerebral artery occlusion (pMCAO). The relative mRNA level of the genes studied was assessed using real-time reverse transcription-PCR. The Vegf-b and Vegf-d genes were most affected by the peptides, which resulted in their most noticeable activation at 3 h after pMCAO. The level of Vegf-d transcripts decreased considerably, whereas the mRNA level of the Vegf-b gene was significantly increased after 72 h of treatment with each of the peptides. In addition, the effects of the peptides on the expression of the Vegf-b and Vegf-d genes were the opposite of the action of ischemia. It is suggested that the identified effects of the peptides diminish the effects of ischemia, thus participating in the positive therapeutic effect of Semax on ischemic stroke.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Medvedeva EV
Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182 Kurchatov sq. 2, Moscow, Russia. medvedevaekaterina@yandex.ru
Dmitrieva VG
No affiliation info available
Povarova OV
No affiliation info available
Limborska SA
No affiliation info available
Skvortsova VI
No affiliation info available
Myasoedov NF
No affiliation info available
Dergunova LV
No affiliation info available

MeSH

Adrenocorticotropic HormoneAnimalsFrontal LobeInfarction, Middle Cerebral ArteryMaleNeuroprotective AgentsOligopeptidesParietal LobePeptide FragmentsProlineRNA, MessengerRatsRats, WistarReceptors, Vascular Endothelial Growth FactorTranscription, GeneticVascular Endothelial Growth Factor A

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22772900

Citation

Medvedeva, Ekaterina V., et al. "Effect of Semax and Its C-terminal Fragment Pro-Gly-Pro On the Expression of VEGF Family Genes and Their Receptors in Experimental Focal Ischemia of the Rat Brain." Journal of Molecular Neuroscience : MN, vol. 49, no. 2, 2013, pp. 328-33.
Medvedeva EV, Dmitrieva VG, Povarova OV, et al. Effect of semax and its C-terminal fragment Pro-Gly-Pro on the expression of VEGF family genes and their receptors in experimental focal ischemia of the rat brain. J Mol Neurosci. 2013;49(2):328-33.
Medvedeva, E. V., Dmitrieva, V. G., Povarova, O. V., Limborska, S. A., Skvortsova, V. I., Myasoedov, N. F., & Dergunova, L. V. (2013). Effect of semax and its C-terminal fragment Pro-Gly-Pro on the expression of VEGF family genes and their receptors in experimental focal ischemia of the rat brain. Journal of Molecular Neuroscience : MN, 49(2), 328-33. https://doi.org/10.1007/s12031-012-9853-y
Medvedeva EV, et al. Effect of Semax and Its C-terminal Fragment Pro-Gly-Pro On the Expression of VEGF Family Genes and Their Receptors in Experimental Focal Ischemia of the Rat Brain. J Mol Neurosci. 2013;49(2):328-33. PubMed PMID: 22772900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of semax and its C-terminal fragment Pro-Gly-Pro on the expression of VEGF family genes and their receptors in experimental focal ischemia of the rat brain. AU - Medvedeva,Ekaterina V, AU - Dmitrieva,Veronika G, AU - Povarova,Oksana V, AU - Limborska,Svetlana A, AU - Skvortsova,Veronika I, AU - Myasoedov,Nikolay F, AU - Dergunova,Lyudmila V, Y1 - 2012/07/08/ PY - 2012/04/22/received PY - 2012/06/26/accepted PY - 2012/7/10/entrez PY - 2012/7/10/pubmed PY - 2013/6/29/medline SP - 328 EP - 33 JF - Journal of molecular neuroscience : MN JO - J Mol Neurosci VL - 49 IS - 2 N2 - The synthetic peptide Semax (Met-Glu-His-Phe-Pro-Gly-Pro) is used successfully in acute stroke therapy. In spite of numerous studies on the subject, many aspects of the neuroprotective effects of the peptide remain unknown. We studied the action of Semax and its C-terminal tripeptide Pro-Gly-Pro on the expression of the VEGF gene family (Vegf-a, Vegf-b, Vegf-c, Vegf-d, and Plgf) and their receptors (Vegfr-1, Vegfr-2, and Vegfr-3) in the frontoparietal cortex region of the rat brain at 3, 24, and 72 h after permanent left middle cerebral artery occlusion (pMCAO). The relative mRNA level of the genes studied was assessed using real-time reverse transcription-PCR. The Vegf-b and Vegf-d genes were most affected by the peptides, which resulted in their most noticeable activation at 3 h after pMCAO. The level of Vegf-d transcripts decreased considerably, whereas the mRNA level of the Vegf-b gene was significantly increased after 72 h of treatment with each of the peptides. In addition, the effects of the peptides on the expression of the Vegf-b and Vegf-d genes were the opposite of the action of ischemia. It is suggested that the identified effects of the peptides diminish the effects of ischemia, thus participating in the positive therapeutic effect of Semax on ischemic stroke. SN - 1559-1166 UR - https://www.unboundmedicine.com/medline/citation/22772900/Effect_of_semax_and_its_C_terminal_fragment_Pro_Gly_Pro_on_the_expression_of_VEGF_family_genes_and_their_receptors_in_experimental_focal_ischemia_of_the_rat_brain_ L2 - https://dx.doi.org/10.1007/s12031-012-9853-y DB - PRIME DP - Unbound Medicine ER -