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GFAJ-1 is an arsenate-resistant, phosphate-dependent organism.
Science. 2012 Jul 27; 337(6093):467-70.Sci

Abstract

The bacterial isolate GFAJ-1 has been proposed to substitute arsenic for phosphorus to sustain growth. We have shown that GFAJ-1 is able to grow at low phosphate concentrations (1.7 μM), even in the presence of high concentrations of arsenate (40 mM), but lacks the ability to grow in phosphorus-depleted (<0.3 μM), arsenate-containing medium. High-resolution mass spectrometry analyses revealed that phosphorylated central metabolites and phosphorylated nucleic acids predominated. A few arsenylated compounds, including C6 sugar arsenates, were detected in extracts of GFAJ-1, when GFAJ-1 was incubated with arsenate, but further experiments showed they formed abiotically. Inductively coupled plasma mass spectrometry confirmed the presence of phosphorus in nucleic acid extracts, while arsenic could not be detected and was below 1 per mil relative to phosphorus. Taken together, we conclude that GFAJ-1 is an arsenate-resistant, but still a phosphate-dependent, bacterium.

Authors+Show Affiliations

Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland. toerb@ethz.chNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22773139

Citation

Erb, Tobias J., et al. "GFAJ-1 Is an Arsenate-resistant, Phosphate-dependent Organism." Science (New York, N.Y.), vol. 337, no. 6093, 2012, pp. 467-70.
Erb TJ, Kiefer P, Hattendorf B, et al. GFAJ-1 is an arsenate-resistant, phosphate-dependent organism. Science. 2012;337(6093):467-70.
Erb, T. J., Kiefer, P., Hattendorf, B., Günther, D., & Vorholt, J. A. (2012). GFAJ-1 is an arsenate-resistant, phosphate-dependent organism. Science (New York, N.Y.), 337(6093), 467-70. https://doi.org/10.1126/science.1218455
Erb TJ, et al. GFAJ-1 Is an Arsenate-resistant, Phosphate-dependent Organism. Science. 2012 Jul 27;337(6093):467-70. PubMed PMID: 22773139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GFAJ-1 is an arsenate-resistant, phosphate-dependent organism. AU - Erb,Tobias J, AU - Kiefer,Patrick, AU - Hattendorf,Bodo, AU - Günther,Detlef, AU - Vorholt,Julia A, Y1 - 2012/07/08/ PY - 2012/7/10/entrez PY - 2012/7/10/pubmed PY - 2012/8/9/medline SP - 467 EP - 70 JF - Science (New York, N.Y.) JO - Science VL - 337 IS - 6093 N2 - The bacterial isolate GFAJ-1 has been proposed to substitute arsenic for phosphorus to sustain growth. We have shown that GFAJ-1 is able to grow at low phosphate concentrations (1.7 μM), even in the presence of high concentrations of arsenate (40 mM), but lacks the ability to grow in phosphorus-depleted (<0.3 μM), arsenate-containing medium. High-resolution mass spectrometry analyses revealed that phosphorylated central metabolites and phosphorylated nucleic acids predominated. A few arsenylated compounds, including C6 sugar arsenates, were detected in extracts of GFAJ-1, when GFAJ-1 was incubated with arsenate, but further experiments showed they formed abiotically. Inductively coupled plasma mass spectrometry confirmed the presence of phosphorus in nucleic acid extracts, while arsenic could not be detected and was below 1 per mil relative to phosphorus. Taken together, we conclude that GFAJ-1 is an arsenate-resistant, but still a phosphate-dependent, bacterium. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/22773139/GFAJ_1_is_an_arsenate_resistant_phosphate_dependent_organism_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&amp;pmid=22773139 DB - PRIME DP - Unbound Medicine ER -