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Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells.
Science. 2012 Jul 27; 337(6093):470-3.Sci

Abstract

A strain of Halomonas bacteria, GFAJ-1, has been claimed to be able to use arsenate as a nutrient when phosphate is limiting and to specifically incorporate arsenic into its DNA in place of phosphorus. However, we have found that arsenate does not contribute to growth of GFAJ-1 when phosphate is limiting and that DNA purified from cells grown with limiting phosphate and abundant arsenate does not exhibit the spontaneous hydrolysis expected of arsenate ester bonds. Furthermore, mass spectrometry showed that this DNA contains only trace amounts of free arsenate and no detectable covalently bound arsenate.

Authors+Show Affiliations

Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22773140

Citation

Reaves, Marshall Louis, et al. "Absence of Detectable Arsenate in DNA From Arsenate-grown GFAJ-1 Cells." Science (New York, N.Y.), vol. 337, no. 6093, 2012, pp. 470-3.
Reaves ML, Sinha S, Rabinowitz JD, et al. Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells. Science. 2012;337(6093):470-3.
Reaves, M. L., Sinha, S., Rabinowitz, J. D., Kruglyak, L., & Redfield, R. J. (2012). Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells. Science (New York, N.Y.), 337(6093), 470-3. https://doi.org/10.1126/science.1219861
Reaves ML, et al. Absence of Detectable Arsenate in DNA From Arsenate-grown GFAJ-1 Cells. Science. 2012 Jul 27;337(6093):470-3. PubMed PMID: 22773140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells. AU - Reaves,Marshall Louis, AU - Sinha,Sunita, AU - Rabinowitz,Joshua D, AU - Kruglyak,Leonid, AU - Redfield,Rosemary J, Y1 - 2012/07/08/ PY - 2012/7/10/entrez PY - 2012/7/10/pubmed PY - 2012/8/9/medline SP - 470 EP - 3 JF - Science (New York, N.Y.) JO - Science VL - 337 IS - 6093 N2 - A strain of Halomonas bacteria, GFAJ-1, has been claimed to be able to use arsenate as a nutrient when phosphate is limiting and to specifically incorporate arsenic into its DNA in place of phosphorus. However, we have found that arsenate does not contribute to growth of GFAJ-1 when phosphate is limiting and that DNA purified from cells grown with limiting phosphate and abundant arsenate does not exhibit the spontaneous hydrolysis expected of arsenate ester bonds. Furthermore, mass spectrometry showed that this DNA contains only trace amounts of free arsenate and no detectable covalently bound arsenate. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/22773140/Absence_of_detectable_arsenate_in_DNA_from_arsenate_grown_GFAJ_1_cells_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=22773140 DB - PRIME DP - Unbound Medicine ER -