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Omega-3 fatty acids status in human subjects estimated using a food frequency questionnaire and plasma phospholipids levels.
Nutr J 2012; 11:46NJ

Abstract

BACKGROUND

Intakes of omega-3 (n-3) fatty acids (FA) are associated with several health benefits. The aim of this study was to verify whether intakes of n-3 FA estimated from a food frequency questionnaire (FFQ) correlate with n-3 FA levels measured in plasma phospholipids (PL).

METHODS

The study sample consisted of 200 French-Canadians men and women aged between 18 to 55 years. Dietary data were collected using a validated FFQ. Fasting blood samples were collected and the plasma PL FA profile was measured by gas chromatography.

RESULTS

Low intakes of n-3 long-chain FA together with low percentages of n-3 long-chain FA in plasma PL were found in French-Canadian population. Daily intakes of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) were similar between men and women. Yet, alpha-linolenic acid (ALA) and total n-3 FA intakes were significantly higher in men compared to women (ALA: 2.28 g and 1.69 g, p < 0.0001, total n-3 FA: 2.57 g and 1.99 g, p < 0.0001; respectively). In plasma PL, DPA and DHA percentages were significantly different between men and women (DPA: 1.03% and 0.88%, p < 0.0001, DHA: 3.00% and 3.43%, p = 0.0005; respectively). Moreover, DHA (men: r = 0.52, p < 0.0001; women: r = 0.57, p < 0.0001) and total n-3 FA (men: r = 0.47, p < 0.0001; women: r = 0.52, p < 0.0001) intakes were positively correlated to their respective plasma PL FA levels. In women, EPA (r = 0.44, p < 0.0001) and DPA (r = 0.23, p = 0.02) intakes were also correlated respectively with EPA and DPA plasma PL FA percentages.

CONCLUSION

Estimated n-3 long-chain FA intake among this young and well-educated French-Canadian population is lower than the recommendations. Further, FFQ data is comparable to plasma PL results to estimate DHA and total n-3 FA status in healthy individuals as well as to evaluate the EPA and DPA status in women. Overall, this FFQ could be used as a simple, low-cost tool in future studies to rank n-3 FA status of individuals.

Authors+Show Affiliations

Department of Food Science and Nutrition, Laval University, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22775977

Citation

Garneau, Véronique, et al. "Omega-3 Fatty Acids Status in Human Subjects Estimated Using a Food Frequency Questionnaire and Plasma Phospholipids Levels." Nutrition Journal, vol. 11, 2012, p. 46.
Garneau V, Rudkowska I, Paradis AM, et al. Omega-3 fatty acids status in human subjects estimated using a food frequency questionnaire and plasma phospholipids levels. Nutr J. 2012;11:46.
Garneau, V., Rudkowska, I., Paradis, A. M., Godin, G., Julien, P., Pérusse, L., & Vohl, M. C. (2012). Omega-3 fatty acids status in human subjects estimated using a food frequency questionnaire and plasma phospholipids levels. Nutrition Journal, 11, p. 46. doi:10.1186/1475-2891-11-46.
Garneau V, et al. Omega-3 Fatty Acids Status in Human Subjects Estimated Using a Food Frequency Questionnaire and Plasma Phospholipids Levels. Nutr J. 2012 Jul 9;11:46. PubMed PMID: 22775977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Omega-3 fatty acids status in human subjects estimated using a food frequency questionnaire and plasma phospholipids levels. AU - Garneau,Véronique, AU - Rudkowska,Iwona, AU - Paradis,Ann-Marie, AU - Godin,Gaston, AU - Julien,Pierre, AU - Pérusse,Louis, AU - Vohl,Marie-Claude, Y1 - 2012/07/09/ PY - 2011/11/17/received PY - 2012/07/09/accepted PY - 2012/7/11/entrez PY - 2012/7/11/pubmed PY - 2012/12/21/medline SP - 46 EP - 46 JF - Nutrition journal JO - Nutr J VL - 11 N2 - BACKGROUND: Intakes of omega-3 (n-3) fatty acids (FA) are associated with several health benefits. The aim of this study was to verify whether intakes of n-3 FA estimated from a food frequency questionnaire (FFQ) correlate with n-3 FA levels measured in plasma phospholipids (PL). METHODS: The study sample consisted of 200 French-Canadians men and women aged between 18 to 55 years. Dietary data were collected using a validated FFQ. Fasting blood samples were collected and the plasma PL FA profile was measured by gas chromatography. RESULTS: Low intakes of n-3 long-chain FA together with low percentages of n-3 long-chain FA in plasma PL were found in French-Canadian population. Daily intakes of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) were similar between men and women. Yet, alpha-linolenic acid (ALA) and total n-3 FA intakes were significantly higher in men compared to women (ALA: 2.28 g and 1.69 g, p < 0.0001, total n-3 FA: 2.57 g and 1.99 g, p < 0.0001; respectively). In plasma PL, DPA and DHA percentages were significantly different between men and women (DPA: 1.03% and 0.88%, p < 0.0001, DHA: 3.00% and 3.43%, p = 0.0005; respectively). Moreover, DHA (men: r = 0.52, p < 0.0001; women: r = 0.57, p < 0.0001) and total n-3 FA (men: r = 0.47, p < 0.0001; women: r = 0.52, p < 0.0001) intakes were positively correlated to their respective plasma PL FA levels. In women, EPA (r = 0.44, p < 0.0001) and DPA (r = 0.23, p = 0.02) intakes were also correlated respectively with EPA and DPA plasma PL FA percentages. CONCLUSION: Estimated n-3 long-chain FA intake among this young and well-educated French-Canadian population is lower than the recommendations. Further, FFQ data is comparable to plasma PL results to estimate DHA and total n-3 FA status in healthy individuals as well as to evaluate the EPA and DPA status in women. Overall, this FFQ could be used as a simple, low-cost tool in future studies to rank n-3 FA status of individuals. SN - 1475-2891 UR - https://www.unboundmedicine.com/medline/citation/22775977/Omega_3_fatty_acids_status_in_human_subjects_estimated_using_a_food_frequency_questionnaire_and_plasma_phospholipids_levels_ L2 - https://nutritionj.biomedcentral.com/articles/10.1186/1475-2891-11-46 DB - PRIME DP - Unbound Medicine ER -