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Reduced satellite cell numbers with spinal cord injury and aging in humans.
Med Sci Sports Exerc. 2012 Dec; 44(12):2322-30.MS

Abstract

INTRODUCTION

Both sarcopenia and spinal cord injury (SCI) are characterized by the loss of skeletal muscle mass and function. Despite obvious similarities in atrophy between both models, differences in muscle fiber size and satellite cell content may exist on a muscle fiber type-specific level.

METHODS

In the present study, we compared skeletal muscle fiber characteristics between wheelchair-dependent young males with SCI (n = 8, 32 ± 4 yr), healthy elderly males (n = 8, 75 ± 2 yr), and young controls (n = 8, 31 ± 3 yr). Muscle biopsies were collected to determine skeletal muscle fiber type composition, fiber size, and satellite cell content.

RESULTS

Severe atrophy and a shift toward approximately 90% Type II muscle fibers were observed in muscle obtained from males with SCI. Muscle fiber size was substantially smaller in both the SCI (Types I and II fibers) and elderly subjects (Type II fibers) when compared with the controls. Satellite cell content was substantially lower in the wheelchair-dependent SCI subjects in both the Types I and II muscle fibers (0.049 ± 0.019 and 0.050 ± 0.005 satellite cells per fiber, respectively) when compared with the young controls (0.104 ± 0.011 and 0.117 ± 0.009 satellite cells per fiber, respectively). In the elderly, the number of satellite cells was lower in the Type II muscle fibers only (0.042 ± 0.005 vs 0.117 ± 0.009 satellite cells per fiber in the elderly vs young controls, respectively).

CONCLUSION

This is the first study to show that muscle fiber atrophy as observed with SCI (Types I and II fibers) and aging (Type II fibers) is accompanied by a muscle fiber type-specific reduction in satellite cell content in humans.

Authors+Show Affiliations

NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Movement Sciences, Maastricht University Medical Centre+, Maastricht, The Netherlands. lex.verdijk@maastrichtuniversity.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22776875

Citation

Verdijk, Lex B., et al. "Reduced Satellite Cell Numbers With Spinal Cord Injury and Aging in Humans." Medicine and Science in Sports and Exercise, vol. 44, no. 12, 2012, pp. 2322-30.
Verdijk LB, Dirks ML, Snijders T, et al. Reduced satellite cell numbers with spinal cord injury and aging in humans. Med Sci Sports Exerc. 2012;44(12):2322-30.
Verdijk, L. B., Dirks, M. L., Snijders, T., Prompers, J. J., Beelen, M., Jonkers, R. A., Thijssen, D. H., Hopman, M. T., & Van Loon, L. J. (2012). Reduced satellite cell numbers with spinal cord injury and aging in humans. Medicine and Science in Sports and Exercise, 44(12), 2322-30. https://doi.org/10.1249/MSS.0b013e3182667c2e
Verdijk LB, et al. Reduced Satellite Cell Numbers With Spinal Cord Injury and Aging in Humans. Med Sci Sports Exerc. 2012;44(12):2322-30. PubMed PMID: 22776875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced satellite cell numbers with spinal cord injury and aging in humans. AU - Verdijk,Lex B, AU - Dirks,Marlou L, AU - Snijders,Tim, AU - Prompers,Jeanine J, AU - Beelen,Milou, AU - Jonkers,Richard A M, AU - Thijssen,Dick H J, AU - Hopman,Maria T E, AU - Van Loon,Luc J C, PY - 2012/7/11/entrez PY - 2012/7/11/pubmed PY - 2013/5/11/medline SP - 2322 EP - 30 JF - Medicine and science in sports and exercise JO - Med Sci Sports Exerc VL - 44 IS - 12 N2 - INTRODUCTION: Both sarcopenia and spinal cord injury (SCI) are characterized by the loss of skeletal muscle mass and function. Despite obvious similarities in atrophy between both models, differences in muscle fiber size and satellite cell content may exist on a muscle fiber type-specific level. METHODS: In the present study, we compared skeletal muscle fiber characteristics between wheelchair-dependent young males with SCI (n = 8, 32 ± 4 yr), healthy elderly males (n = 8, 75 ± 2 yr), and young controls (n = 8, 31 ± 3 yr). Muscle biopsies were collected to determine skeletal muscle fiber type composition, fiber size, and satellite cell content. RESULTS: Severe atrophy and a shift toward approximately 90% Type II muscle fibers were observed in muscle obtained from males with SCI. Muscle fiber size was substantially smaller in both the SCI (Types I and II fibers) and elderly subjects (Type II fibers) when compared with the controls. Satellite cell content was substantially lower in the wheelchair-dependent SCI subjects in both the Types I and II muscle fibers (0.049 ± 0.019 and 0.050 ± 0.005 satellite cells per fiber, respectively) when compared with the young controls (0.104 ± 0.011 and 0.117 ± 0.009 satellite cells per fiber, respectively). In the elderly, the number of satellite cells was lower in the Type II muscle fibers only (0.042 ± 0.005 vs 0.117 ± 0.009 satellite cells per fiber in the elderly vs young controls, respectively). CONCLUSION: This is the first study to show that muscle fiber atrophy as observed with SCI (Types I and II fibers) and aging (Type II fibers) is accompanied by a muscle fiber type-specific reduction in satellite cell content in humans. SN - 1530-0315 UR - https://www.unboundmedicine.com/medline/citation/22776875/Reduced_satellite_cell_numbers_with_spinal_cord_injury_and_aging_in_humans_ L2 - https://doi.org/10.1249/MSS.0b013e3182667c2e DB - PRIME DP - Unbound Medicine ER -