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[Study on the oxidative stress and inflammation in trophoblast cells stimulated by different chain length fatty acids].
Zhonghua Fu Chan Ke Za Zhi. 2012 Apr; 47(4):268-73.ZF

Abstract

OBJECTIVE

To investigate the oxidative stress and inflammation in trophoblast cells stimulated by different chain length fatty acids.

METHODS

Serum-free trophoblast cells cultured in vitro were divided into five groups, which were incubated with DMEM medium without free fatty acid (F-FFA), short chain fatty acids (SC-FFA), medium chain fatty acids (MC-FFA), long chain fatty acids (LC-FFA), very long chain fatty acids (VLC-FFA). Then cells in each group were stimulated by DMEM medium, reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor (apocynin) and p38 mitogen-activated protein kinases (p38MAPK) inhibitor (SB203580) and were subdivided as each FFA plus-DMEM group, plus-NADPH-I and plus-p38MAPK-I groups. Expressions of mRNA and protein of p38MAPK and cyclooxygenase 2 (COX-2) in trophoblast cells were detected by real-time PCR and western blot.

RESULTS

(1) The mRNA expression of p38MAPK in LC-FFA + DMEM, VLC-FFA + DMEM, LC-FFA + NADPH-I, LC-FFA + p38MAPK-I, VLC-FFA + NADPH-I, VLC-FFA + p38MAPK-I group were 4.56 ± 0.28, 22.65 ± 2.40, 0.87 ± 0.06, 1.02 ± 0.15, 19.87 ± 1.93, 10.22 ± 0.75 separately, and the protein expressions were 0.79 ± 0.02, 0.93 ± 0.10, 0.43 ± 0.06, 0.44 ± 0.19, 0.79 ± 0.10, 0.81 ± 0.14. Compared with other groups, the mRNA and protein expressions of p38MAPK in LC-FFA + DMEM, VLC-FFA + DMEM group were increased (P < 0.05). Compared with LC-FFA + DMEM group, mRNA and protein expressions of p38MAPK in LC-FFA + NADPH-I and LC-FFA + p38MAPK-I group were significantly decreased (P < 0.05). Compared with VLC-FFA + DMEM group, mRNA and protein expressions of p38MAPK had no difference in VLC-FFA + NADPH-I group (P > 0.05), mRNA expression of p38MAPK in VLC-FFA + p38MAPK-I group was significantly decreased (P < 0.05), but there was no difference in protein expression (P > 0.05). (2) The mRNA expression of COX-2 in LC-FFA + DMEM, VLC-FFA + DMEM, LC-FFA + NADPH-I, LC-FFA + p38MAPK-I, VLC-FFA + NADPH-I, VLC-FFA + p38MAPK-I group were 3.97 ± 0.03, 39.08 ± 0.63, 0.99 ± 0.13, 0.98 ± 0.18, 20.93 ± 3.70, 13.46 ± 2.31 separately, and the protein expressions were 1.32 ± 0.20, 1.33 ± 0.25, 0.59 ± 0.13, 0.58 ± 0.30, 0.88 ± 0.18, 0.91 ± 0.24. Compared with other groups, mRNA and protein expressions of COX-2 in LC-FFA + DMEM and VLC-FFA + DMEM group were significantly increased (P < 0.05). Compared with LC-FFA + DMEM group, mRNA and protein expressions of COX-2 in LC-FFA + NADPH-I and LC-FFA + p38MAPK-I group were decreased (P < 0.05). Compared with VLC-FFA + DMEM group, mRNA and protein expressions of COX-2 in VLC-FFA + NADPH-I and VLC-FFA + p38MAPK-I group were all decreased (P < 0.05). (3) The correlation analysis showed that there were significantly positive correlations between the mRNA and protein expressions of p38MAPK and COX-2 in LC-FFA group (P < 0.05). There were significantly positive correlations in protein expression (P < 0.05), but no correlation in the mRNA expression between p38MAPK and COX-2 in the F-FFA, SC-FFA, MC-FFA, VLC-FFA groups (P > 0.05).

CONCLUSIONS

The oxidative stress and inflammation may exist in trophoblast cells which were stimulated by LC-FFA and VLC-FFA. p38MAPK signal transduction pathway may contributed in this process.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22781113

Citation

Sun, Xiao-le, et al. "[Study On the Oxidative Stress and Inflammation in Trophoblast Cells Stimulated By Different Chain Length Fatty Acids]." Zhonghua Fu Chan Ke Za Zhi, vol. 47, no. 4, 2012, pp. 268-73.
Sun XL, Yang Z, Wang XY, et al. [Study on the oxidative stress and inflammation in trophoblast cells stimulated by different chain length fatty acids]. Zhonghua Fu Chan Ke Za Zhi. 2012;47(4):268-73.
Sun, X. L., Yang, Z., Wang, X. Y., & Wang, J. L. (2012). [Study on the oxidative stress and inflammation in trophoblast cells stimulated by different chain length fatty acids]. Zhonghua Fu Chan Ke Za Zhi, 47(4), 268-73.
Sun XL, et al. [Study On the Oxidative Stress and Inflammation in Trophoblast Cells Stimulated By Different Chain Length Fatty Acids]. Zhonghua Fu Chan Ke Za Zhi. 2012;47(4):268-73. PubMed PMID: 22781113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on the oxidative stress and inflammation in trophoblast cells stimulated by different chain length fatty acids]. AU - Sun,Xiao-le, AU - Yang,Zi, AU - Wang,Xiao-Ye, AU - Wang,Jia-Lüe, PY - 2012/7/12/entrez PY - 2012/7/12/pubmed PY - 2013/2/16/medline SP - 268 EP - 73 JF - Zhonghua fu chan ke za zhi JO - Zhonghua Fu Chan Ke Za Zhi VL - 47 IS - 4 N2 - OBJECTIVE: To investigate the oxidative stress and inflammation in trophoblast cells stimulated by different chain length fatty acids. METHODS: Serum-free trophoblast cells cultured in vitro were divided into five groups, which were incubated with DMEM medium without free fatty acid (F-FFA), short chain fatty acids (SC-FFA), medium chain fatty acids (MC-FFA), long chain fatty acids (LC-FFA), very long chain fatty acids (VLC-FFA). Then cells in each group were stimulated by DMEM medium, reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor (apocynin) and p38 mitogen-activated protein kinases (p38MAPK) inhibitor (SB203580) and were subdivided as each FFA plus-DMEM group, plus-NADPH-I and plus-p38MAPK-I groups. Expressions of mRNA and protein of p38MAPK and cyclooxygenase 2 (COX-2) in trophoblast cells were detected by real-time PCR and western blot. RESULTS: (1) The mRNA expression of p38MAPK in LC-FFA + DMEM, VLC-FFA + DMEM, LC-FFA + NADPH-I, LC-FFA + p38MAPK-I, VLC-FFA + NADPH-I, VLC-FFA + p38MAPK-I group were 4.56 ± 0.28, 22.65 ± 2.40, 0.87 ± 0.06, 1.02 ± 0.15, 19.87 ± 1.93, 10.22 ± 0.75 separately, and the protein expressions were 0.79 ± 0.02, 0.93 ± 0.10, 0.43 ± 0.06, 0.44 ± 0.19, 0.79 ± 0.10, 0.81 ± 0.14. Compared with other groups, the mRNA and protein expressions of p38MAPK in LC-FFA + DMEM, VLC-FFA + DMEM group were increased (P < 0.05). Compared with LC-FFA + DMEM group, mRNA and protein expressions of p38MAPK in LC-FFA + NADPH-I and LC-FFA + p38MAPK-I group were significantly decreased (P < 0.05). Compared with VLC-FFA + DMEM group, mRNA and protein expressions of p38MAPK had no difference in VLC-FFA + NADPH-I group (P > 0.05), mRNA expression of p38MAPK in VLC-FFA + p38MAPK-I group was significantly decreased (P < 0.05), but there was no difference in protein expression (P > 0.05). (2) The mRNA expression of COX-2 in LC-FFA + DMEM, VLC-FFA + DMEM, LC-FFA + NADPH-I, LC-FFA + p38MAPK-I, VLC-FFA + NADPH-I, VLC-FFA + p38MAPK-I group were 3.97 ± 0.03, 39.08 ± 0.63, 0.99 ± 0.13, 0.98 ± 0.18, 20.93 ± 3.70, 13.46 ± 2.31 separately, and the protein expressions were 1.32 ± 0.20, 1.33 ± 0.25, 0.59 ± 0.13, 0.58 ± 0.30, 0.88 ± 0.18, 0.91 ± 0.24. Compared with other groups, mRNA and protein expressions of COX-2 in LC-FFA + DMEM and VLC-FFA + DMEM group were significantly increased (P < 0.05). Compared with LC-FFA + DMEM group, mRNA and protein expressions of COX-2 in LC-FFA + NADPH-I and LC-FFA + p38MAPK-I group were decreased (P < 0.05). Compared with VLC-FFA + DMEM group, mRNA and protein expressions of COX-2 in VLC-FFA + NADPH-I and VLC-FFA + p38MAPK-I group were all decreased (P < 0.05). (3) The correlation analysis showed that there were significantly positive correlations between the mRNA and protein expressions of p38MAPK and COX-2 in LC-FFA group (P < 0.05). There were significantly positive correlations in protein expression (P < 0.05), but no correlation in the mRNA expression between p38MAPK and COX-2 in the F-FFA, SC-FFA, MC-FFA, VLC-FFA groups (P > 0.05). CONCLUSIONS: The oxidative stress and inflammation may exist in trophoblast cells which were stimulated by LC-FFA and VLC-FFA. p38MAPK signal transduction pathway may contributed in this process. SN - 0529-567X UR - https://www.unboundmedicine.com/medline/citation/22781113/[Study_on_the_oxidative_stress_and_inflammation_in_trophoblast_cells_stimulated_by_different_chain_length_fatty_acids]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0529-567X&amp;year=2012&amp;vol=47&amp;issue=4&amp;fpage=268 DB - PRIME DP - Unbound Medicine ER -