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Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by L-NAME in rats.
Life Sci. 2012 Sep 04; 91(5-6):159-65.LS

Abstract

AIMS

Inhibition of nitric oxide synthase with N-omega-nitro-l-arginine methyl ester (l-NAME) has been employed as an experimental model of human preeclampsia. This study determined the protective effect of silibinin, a flavonoid with anti-inflammatory and hepatoprotective properties on the deleterious effects observed in experimentally induced preeclampsia in rats.

MAIN METHODS

Pregnant Wistar rats were treated during gestation (days 10-20) with l-NAME (70-80mg/kg/day) in drinking water or with l-NAME plus silibinin (100mg/kg/day, orally) starting at day 0, day 7 or day 14 of pregnancy. Systolic blood pressure was recorded from gestation days 0 to 21. A control group of pregnant non-treated rats was analyzed similarly. On day 21 the rats were euthanized and the following parameters were evaluated: proteinuria, platelet count, liver histopathology and reproductive outcome. Tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1β), IL-6, IL-10 and interferon-gamma (IFN-γ) were determined in liver homogenate by enzyme immunoassay.

KEY FINDINGS

In comparison with the l-NAME group the silibinin treatment reduced the values of systolic blood pressure, proteinuria, TNF-α, IL-1β and IFN-γ in liver, normalized the platelet count and improved fetal outcomes. Histopathological lesions in liver of the l-NAME group showed intense mononuclear inflammatory infiltrate and thickening of muscle tunica of arterial vessel, mainly in the periportal area. Silibinin treatment induced attenuation of periportal inflammatory infiltrate, showing an association between inflammatory infiltrate and TNF-α, IL-1β and IFN-γ levels in liver homogenate.

SIGNIFICANCE

Silibinin administration to l-NAME-treated rats displays anti-inflammatory and immunomodulatory actions that may contribute to its hepatoprotective effects and improve reproductive outcomes in experimental preeclampsia.

Authors+Show Affiliations

Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University, Botucatu, São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22781706

Citation

Souza, Camila Oliveira de, et al. "Hepatoprotective and Anti-inflammatory Effects of Silibinin On Experimental Preeclampsia Induced By L-NAME in Rats." Life Sciences, vol. 91, no. 5-6, 2012, pp. 159-65.
Souza CO, Peraçoli MT, Weel IC, et al. Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by L-NAME in rats. Life Sci. 2012;91(5-6):159-65.
Souza, C. O., Peraçoli, M. T., Weel, I. C., Bannwart, C. F., Romão, M., Nakaira-Takahagi, E., Medeiros, L. T., Silva, M. G., & Peraçoli, J. C. (2012). Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by L-NAME in rats. Life Sciences, 91(5-6), 159-65. https://doi.org/10.1016/j.lfs.2012.06.036
Souza CO, et al. Hepatoprotective and Anti-inflammatory Effects of Silibinin On Experimental Preeclampsia Induced By L-NAME in Rats. Life Sci. 2012 Sep 4;91(5-6):159-65. PubMed PMID: 22781706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by L-NAME in rats. AU - Souza,Camila Oliveira de, AU - Peraçoli,Maria Terezinha Serrão, AU - Weel,Ingrid Cristina, AU - Bannwart,Camila Ferreira, AU - Romão,Mariana, AU - Nakaira-Takahagi,Erika, AU - Medeiros,Leonardo Teixeira Lopes de, AU - Silva,Márcia Guimarães da, AU - Peraçoli,José Carlos, Y1 - 2012/07/07/ PY - 2011/09/01/received PY - 2012/05/31/revised PY - 2012/06/25/accepted PY - 2012/7/12/entrez PY - 2012/7/12/pubmed PY - 2012/10/23/medline SP - 159 EP - 65 JF - Life sciences JO - Life Sci VL - 91 IS - 5-6 N2 - AIMS: Inhibition of nitric oxide synthase with N-omega-nitro-l-arginine methyl ester (l-NAME) has been employed as an experimental model of human preeclampsia. This study determined the protective effect of silibinin, a flavonoid with anti-inflammatory and hepatoprotective properties on the deleterious effects observed in experimentally induced preeclampsia in rats. MAIN METHODS: Pregnant Wistar rats were treated during gestation (days 10-20) with l-NAME (70-80mg/kg/day) in drinking water or with l-NAME plus silibinin (100mg/kg/day, orally) starting at day 0, day 7 or day 14 of pregnancy. Systolic blood pressure was recorded from gestation days 0 to 21. A control group of pregnant non-treated rats was analyzed similarly. On day 21 the rats were euthanized and the following parameters were evaluated: proteinuria, platelet count, liver histopathology and reproductive outcome. Tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1β), IL-6, IL-10 and interferon-gamma (IFN-γ) were determined in liver homogenate by enzyme immunoassay. KEY FINDINGS: In comparison with the l-NAME group the silibinin treatment reduced the values of systolic blood pressure, proteinuria, TNF-α, IL-1β and IFN-γ in liver, normalized the platelet count and improved fetal outcomes. Histopathological lesions in liver of the l-NAME group showed intense mononuclear inflammatory infiltrate and thickening of muscle tunica of arterial vessel, mainly in the periportal area. Silibinin treatment induced attenuation of periportal inflammatory infiltrate, showing an association between inflammatory infiltrate and TNF-α, IL-1β and IFN-γ levels in liver homogenate. SIGNIFICANCE: Silibinin administration to l-NAME-treated rats displays anti-inflammatory and immunomodulatory actions that may contribute to its hepatoprotective effects and improve reproductive outcomes in experimental preeclampsia. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/22781706/Hepatoprotective_and_anti_inflammatory_effects_of_silibinin_on_experimental_preeclampsia_induced_by_L_NAME_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(12)00344-X DB - PRIME DP - Unbound Medicine ER -