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Neurogenic vascular responses in male mouse mesenteric vascular beds.
J Pharmacol Sci. 2012; 119(3):260-70.JP

Abstract

Rat mesenteric arteries were maintained by both adrenergic vasoconstrictor nerves and calcitonin gene-related peptide (CGRP) vasodilator nerves. However, functions of these nerves in a pathophysiological state have not fully been analyzed. The use of disease models developed genetically in mice is expected to clarify neural function of perivascular nerves. Thus, we investigated basic mouse vascular responses. Mesenteric vascular beds isolated from male C57BL/6 mouse were perfused with Krebs solution and perfusion pressure was measured. Periarterial nerve stimulation (PNS, 8 - 24 Hz) induced frequency-dependent vasoconstriction, which increased flow rate-dependently. PNS-induced vasoconstriction was abolished by tetrodotoxin (neurotoxin) and guanethidine (adrenergic neuron blocker) and blunted by prazosin (α(1)-adrenoceptor antagonist). Injection of norepinephrine caused vasoconstriction, which was abolished by prazosin. In preparations with active tone, PNS (1 - 8 Hz) induced frequency-dependent vasodilation, which was inhibited by tetrodotoxin, capsaicin (CGRP depletor), and CGRP8-37 (CGRP-receptor antagonist). Injections of CGRP, acetylcholine, and sodium nitroprusside induced vasodilations. Vasodilator response to CGRP was inhibited by CGRP8-37. Immunohistochemical study showed innervation of tyrosine hydroxylase- and CGRP-immunopositive fibers in mesenteric arteries and veins. These results suggest that male mouse mesenteric vascular beds are useful for studying neural regulation of mesenteric arteries, which are innervated by adrenergic and CGRPergic nerves regulating vascular tone.

Authors+Show Affiliations

Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22785022

Citation

Fujiwara, Hiroki, et al. "Neurogenic Vascular Responses in Male Mouse Mesenteric Vascular Beds." Journal of Pharmacological Sciences, vol. 119, no. 3, 2012, pp. 260-70.
Fujiwara H, Hashikawa-Hobara N, Wake Y, et al. Neurogenic vascular responses in male mouse mesenteric vascular beds. J Pharmacol Sci. 2012;119(3):260-70.
Fujiwara, H., Hashikawa-Hobara, N., Wake, Y., Takatori, S., Goda, M., Higuchi, H., Zamami, Y., Tangsucharit, P., & Kawasaki, H. (2012). Neurogenic vascular responses in male mouse mesenteric vascular beds. Journal of Pharmacological Sciences, 119(3), 260-70.
Fujiwara H, et al. Neurogenic Vascular Responses in Male Mouse Mesenteric Vascular Beds. J Pharmacol Sci. 2012;119(3):260-70. PubMed PMID: 22785022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurogenic vascular responses in male mouse mesenteric vascular beds. AU - Fujiwara,Hiroki, AU - Hashikawa-Hobara,Narumi, AU - Wake,Yoshihiro, AU - Takatori,Shingo, AU - Goda,Mitsuhiro, AU - Higuchi,Hiroshi, AU - Zamami,Yoshito, AU - Tangsucharit,Panot, AU - Kawasaki,Hiromu, Y1 - 2012/06/28/ PY - 2012/7/13/entrez PY - 2012/7/13/pubmed PY - 2013/1/31/medline SP - 260 EP - 70 JF - Journal of pharmacological sciences JO - J Pharmacol Sci VL - 119 IS - 3 N2 - Rat mesenteric arteries were maintained by both adrenergic vasoconstrictor nerves and calcitonin gene-related peptide (CGRP) vasodilator nerves. However, functions of these nerves in a pathophysiological state have not fully been analyzed. The use of disease models developed genetically in mice is expected to clarify neural function of perivascular nerves. Thus, we investigated basic mouse vascular responses. Mesenteric vascular beds isolated from male C57BL/6 mouse were perfused with Krebs solution and perfusion pressure was measured. Periarterial nerve stimulation (PNS, 8 - 24 Hz) induced frequency-dependent vasoconstriction, which increased flow rate-dependently. PNS-induced vasoconstriction was abolished by tetrodotoxin (neurotoxin) and guanethidine (adrenergic neuron blocker) and blunted by prazosin (α(1)-adrenoceptor antagonist). Injection of norepinephrine caused vasoconstriction, which was abolished by prazosin. In preparations with active tone, PNS (1 - 8 Hz) induced frequency-dependent vasodilation, which was inhibited by tetrodotoxin, capsaicin (CGRP depletor), and CGRP8-37 (CGRP-receptor antagonist). Injections of CGRP, acetylcholine, and sodium nitroprusside induced vasodilations. Vasodilator response to CGRP was inhibited by CGRP8-37. Immunohistochemical study showed innervation of tyrosine hydroxylase- and CGRP-immunopositive fibers in mesenteric arteries and veins. These results suggest that male mouse mesenteric vascular beds are useful for studying neural regulation of mesenteric arteries, which are innervated by adrenergic and CGRPergic nerves regulating vascular tone. SN - 1347-8648 UR - https://www.unboundmedicine.com/medline/citation/22785022/Neurogenic_vascular_responses_in_male_mouse_mesenteric_vascular_beds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/DN/JST.JSTAGE/jphs/12014FP DB - PRIME DP - Unbound Medicine ER -