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Syzygium cumini ameliorates insulin resistance and β-cell dysfunction via modulation of PPAR, dyslipidemia, oxidative stress, and TNF-α in type 2 diabetic rats.
J Pharmacol Sci. 2012; 119(3):205-13.JP

Abstract

Syzygium cumini (SC) is well known for its anti-diabetic potential, but the mechanism underlying its amelioration of type 2 diabetes is still elusive. Therefore, for the first time, we investigated whether SC aqueous seed extract (100, 200, or 400 mg/kg) exerts any beneficial effects on insulin resistance (IR), serum lipid profile, antioxidant status, and/or pancreatic β-cell damage in high-fat diet / streptozotocin-induced (HFD-STZ) diabetic rats. Wistar albino rats were fed with HFD (55% of calories as fat) during the experiment to induce IR and on the 10th day were injected with STZ (40 mg/kg, i.p.) to develop type 2 diabetes. Subsequently, after confirmation of hyperglycemia on the 14th day (fasting glucose level > 13.89 mM), diabetic rats were treated with SC for the next 21 days. Diabetic rats showed increased serum glucose, insulin, IR, TNF-α, dyslipidemia, and pancreatic thiobarbituric acid-reactive substances with a concomitant decrease in β-cell function and pancreatic superoxide dismutase, catalase, and glutathione peroxidase antioxidant enzyme activities. Microscopic examination of their pancreas revealed pathological changes in islets and β-cells. These alterations reverted to near-normal levels after treatment with SC at 400 mg/kg. Moreover, hepatic tissue demonstrated increased PPARγ and PPARα protein expressions. Thus, our study demonstrated the beneficial effect of SC seed extract on IR and β-cell dysfunction in HFD-STZ-induced type 2 diabetic rats.

Authors+Show Affiliations

Cardiovascular and Diabetes Research Laboratory, Department of Pharmacology, All India Institute of Medical Sciences, New Delhi-110029, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22786584

Citation

Sharma, Ashok Kumar, et al. "Syzygium Cumini Ameliorates Insulin Resistance and Β-cell Dysfunction Via Modulation of PPAR, Dyslipidemia, Oxidative Stress, and TNF-α in Type 2 Diabetic Rats." Journal of Pharmacological Sciences, vol. 119, no. 3, 2012, pp. 205-13.
Sharma AK, Bharti S, Kumar R, et al. Syzygium cumini ameliorates insulin resistance and β-cell dysfunction via modulation of PPAR, dyslipidemia, oxidative stress, and TNF-α in type 2 diabetic rats. J Pharmacol Sci. 2012;119(3):205-13.
Sharma, A. K., Bharti, S., Kumar, R., Krishnamurthy, B., Bhatia, J., Kumari, S., & Arya, D. S. (2012). Syzygium cumini ameliorates insulin resistance and β-cell dysfunction via modulation of PPAR, dyslipidemia, oxidative stress, and TNF-α in type 2 diabetic rats. Journal of Pharmacological Sciences, 119(3), 205-13.
Sharma AK, et al. Syzygium Cumini Ameliorates Insulin Resistance and Β-cell Dysfunction Via Modulation of PPAR, Dyslipidemia, Oxidative Stress, and TNF-α in Type 2 Diabetic Rats. J Pharmacol Sci. 2012;119(3):205-13. PubMed PMID: 22786584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Syzygium cumini ameliorates insulin resistance and β-cell dysfunction via modulation of PPAR, dyslipidemia, oxidative stress, and TNF-α in type 2 diabetic rats. AU - Sharma,Ashok Kumar, AU - Bharti,Saurabh, AU - Kumar,Rajiv, AU - Krishnamurthy,Bhaskar, AU - Bhatia,Jagriti, AU - Kumari,Santosh, AU - Arya,Dharamvir Singh, Y1 - 2012/06/21/ PY - 2012/7/13/entrez PY - 2012/7/13/pubmed PY - 2013/1/31/medline SP - 205 EP - 13 JF - Journal of pharmacological sciences JO - J Pharmacol Sci VL - 119 IS - 3 N2 - Syzygium cumini (SC) is well known for its anti-diabetic potential, but the mechanism underlying its amelioration of type 2 diabetes is still elusive. Therefore, for the first time, we investigated whether SC aqueous seed extract (100, 200, or 400 mg/kg) exerts any beneficial effects on insulin resistance (IR), serum lipid profile, antioxidant status, and/or pancreatic β-cell damage in high-fat diet / streptozotocin-induced (HFD-STZ) diabetic rats. Wistar albino rats were fed with HFD (55% of calories as fat) during the experiment to induce IR and on the 10th day were injected with STZ (40 mg/kg, i.p.) to develop type 2 diabetes. Subsequently, after confirmation of hyperglycemia on the 14th day (fasting glucose level > 13.89 mM), diabetic rats were treated with SC for the next 21 days. Diabetic rats showed increased serum glucose, insulin, IR, TNF-α, dyslipidemia, and pancreatic thiobarbituric acid-reactive substances with a concomitant decrease in β-cell function and pancreatic superoxide dismutase, catalase, and glutathione peroxidase antioxidant enzyme activities. Microscopic examination of their pancreas revealed pathological changes in islets and β-cells. These alterations reverted to near-normal levels after treatment with SC at 400 mg/kg. Moreover, hepatic tissue demonstrated increased PPARγ and PPARα protein expressions. Thus, our study demonstrated the beneficial effect of SC seed extract on IR and β-cell dysfunction in HFD-STZ-induced type 2 diabetic rats. SN - 1347-8648 UR - https://www.unboundmedicine.com/medline/citation/22786584/Syzygium_cumini_ameliorates_insulin_resistance_and_β_cell_dysfunction_via_modulation_of_PPAR_dyslipidemia_oxidative_stress_and_TNF_α_in_type_2_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/DN/JST.JSTAGE/jphs/11184FP DB - PRIME DP - Unbound Medicine ER -