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Function of chloride intracellular channel 1 in gastric cancer cells.
World J Gastroenterol. 2012 Jun 28; 18(24):3070-80.WJ

Abstract

AIM

To investigate the effect of chloride intracellular channel 1 (CLIC1) on the cell proliferation, apoptosis, migration and invasion of gastric cancer cells.

METHODS

CLIC1 expression was evaluated in human gastric cancer cell lines SGC-7901 and MGC-803 by real time polymerase chain reaction (RT-PCR). Four segments of small interference RNA (siRNA) targeting CLIC1 mRNA and a no-sense control segment were designed by bioinformatics technology. CLIC1 siRNA was selected using Lipofectamine 2000 and transfected transiently into human gastric cancer SGC-7901 and MGC-803 cells. The transfected efficiency was observed under fluorescence microscope. After transfection, mRNA expression of CLIC1 was detected with RT-PCR and Western blotting was used to detect the protein expression. Proliferation was examined by methyl thiazolyl tetrazolium and apoptosis was detected with flow cytometry. Polycarbonate membrane transwell chamber and Matrigel were used for the detection of the changes of invasion and migration of the two cell lines.

RESULTS

In gastric cancer cell lines SGC-7901 and MGC-803, CLIC1 was obviously expressed and CLIC1 siRNA could effectively suppress the expression of CLIC1 protein and mRNA. Proliferation of cells transfected with CLIC1 siRNA3 was enhanced notably, and the highest proliferation rate was 23.3% (P = 0.002) in SGC-7901 and 35.55% (P = 0.001) in MGC-803 cells at 48 h. The G2/M phase proportion increased, while G0/G1 and S phase proportions decreased. The apoptotic rate of the CLIC1 siRNA3 group obviously decreased in both SGC-7901 cells (62.24%, P = 0.000) and MGC-803 cells (52.67%, P = 0.004). Down-regulation of CLIC1 led to the inhibition of invasion and migration by 54.31% (P = 0.000) and 33.62% (P = 0.001) in SGC-7901 and 40.74% (P = 0.000) and 29.26% (P = 0.002) in MGC-803. However, there was no significant difference between the mock group cells and the negative control group cells.

CONCLUSION

High CLIC1 expression can efficiently inhibit proliferation and enhance apoptosis, migration and invasion of gastric cancer cells in vitro. CLIC1 might be a promising target for the treatment of gastric cancer.

Authors+Show Affiliations

Department of Gastrointestinal Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22791942

Citation

Ma, Peng-Fei, et al. "Function of Chloride Intracellular Channel 1 in Gastric Cancer Cells." World Journal of Gastroenterology, vol. 18, no. 24, 2012, pp. 3070-80.
Ma PF, Chen JQ, Wang Z, et al. Function of chloride intracellular channel 1 in gastric cancer cells. World J Gastroenterol. 2012;18(24):3070-80.
Ma, P. F., Chen, J. Q., Wang, Z., Liu, J. L., & Li, B. P. (2012). Function of chloride intracellular channel 1 in gastric cancer cells. World Journal of Gastroenterology, 18(24), 3070-80. https://doi.org/10.3748/wjg.v18.i24.3070
Ma PF, et al. Function of Chloride Intracellular Channel 1 in Gastric Cancer Cells. World J Gastroenterol. 2012 Jun 28;18(24):3070-80. PubMed PMID: 22791942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Function of chloride intracellular channel 1 in gastric cancer cells. AU - Ma,Peng-Fei, AU - Chen,Jun-Qiang, AU - Wang,Zhen, AU - Liu,Jin-Lu, AU - Li,Bo-Pei, PY - 2011/12/29/received PY - 2012/02/28/revised PY - 2012/04/09/accepted PY - 2012/7/14/entrez PY - 2012/7/14/pubmed PY - 2012/12/10/medline KW - Apoptosis KW - Chloride intracellular channel 1 KW - Gastric carcinoma KW - Invasion KW - Migration KW - Small interference RNA SP - 3070 EP - 80 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 18 IS - 24 N2 - AIM: To investigate the effect of chloride intracellular channel 1 (CLIC1) on the cell proliferation, apoptosis, migration and invasion of gastric cancer cells. METHODS: CLIC1 expression was evaluated in human gastric cancer cell lines SGC-7901 and MGC-803 by real time polymerase chain reaction (RT-PCR). Four segments of small interference RNA (siRNA) targeting CLIC1 mRNA and a no-sense control segment were designed by bioinformatics technology. CLIC1 siRNA was selected using Lipofectamine 2000 and transfected transiently into human gastric cancer SGC-7901 and MGC-803 cells. The transfected efficiency was observed under fluorescence microscope. After transfection, mRNA expression of CLIC1 was detected with RT-PCR and Western blotting was used to detect the protein expression. Proliferation was examined by methyl thiazolyl tetrazolium and apoptosis was detected with flow cytometry. Polycarbonate membrane transwell chamber and Matrigel were used for the detection of the changes of invasion and migration of the two cell lines. RESULTS: In gastric cancer cell lines SGC-7901 and MGC-803, CLIC1 was obviously expressed and CLIC1 siRNA could effectively suppress the expression of CLIC1 protein and mRNA. Proliferation of cells transfected with CLIC1 siRNA3 was enhanced notably, and the highest proliferation rate was 23.3% (P = 0.002) in SGC-7901 and 35.55% (P = 0.001) in MGC-803 cells at 48 h. The G2/M phase proportion increased, while G0/G1 and S phase proportions decreased. The apoptotic rate of the CLIC1 siRNA3 group obviously decreased in both SGC-7901 cells (62.24%, P = 0.000) and MGC-803 cells (52.67%, P = 0.004). Down-regulation of CLIC1 led to the inhibition of invasion and migration by 54.31% (P = 0.000) and 33.62% (P = 0.001) in SGC-7901 and 40.74% (P = 0.000) and 29.26% (P = 0.002) in MGC-803. However, there was no significant difference between the mock group cells and the negative control group cells. CONCLUSION: High CLIC1 expression can efficiently inhibit proliferation and enhance apoptosis, migration and invasion of gastric cancer cells in vitro. CLIC1 might be a promising target for the treatment of gastric cancer. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/22791942/Function_of_chloride_intracellular_channel_1_in_gastric_cancer_cells_ L2 - https://www.wjgnet.com/1007-9327/full/v18/i24/3070.htm DB - PRIME DP - Unbound Medicine ER -