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[Quickly investigating the absorption ingredients of Gentianae Radix et Rhizoma by SEMAC].
Zhongguo Zhong Yao Za Zhi. 2012 Apr; 37(7):969-73.ZZ

Abstract

OBJECTIVE

To establish a method for quickly investigating the absorption ingredients which could be used as the index of quality control of Gentianae Radix et Rhizoma.

METHOD

The absorption ingredients of Gentianae Radix et Rhizoma were investigated by using the model of in vitro everted intestinal sac (VEIS). The intestinal sac liquors of jejunum and ileum were collected at 6 intervals (15, 30, 45, 60, 90, 120 min) and gentiopicroside, loganin acid, swertiamarin and sweroside were detected by HPLC as the representative marker. The accumulative absorption quantity of gentiopicroside, loganin acid, swertiamarin and sweroside were calculated, respectively.

RESULT

Six components could be detected in intestinal sac. In different concentrations of Gentianae Radix et Rhizoma, gentiopicroside and swertiamarin in various intestinal sections were the linear absorption (R2 > 0.9), conformed to zero order absorption rate. In jejunum the constant of absorption rate (Ka) of gentiopicroside and swertiamarin increased with the raised dosage of Gentianae Radix et Rhizoma (P < 0.05), which indicated a passive absorption manner, and the value of Ka of high and middle dosage of those in ileum were higher than that of low dosage, and the difference of Ka between high and middle dosage were not significant, which indicated a positive absorption manner. The Ka of high and middle dosage of sweroside in ileum and jejunum were higher than that of low dosage (P < 0.05), and the difference of Ka between high and middle dosage were not significant, which indicated a positive absorption manner. The Ka of loganin acid in jejunum and ileum increased along with the raised dosage of Gentianae Radix et Rhizoma (P < 0.05), which indicated a passive absorption manner.

CONCLUSION

SEMAC could be used as a tool to investigate the absorption ingredients of Gentianae Radix et Rhizoma. Drug in intestine sac was selective, and the absorption part of intestine was also different.

Authors+Show Affiliations

Song B
Shaanxi University of Chinese Medicine, Xi'an 712046, China.
Zhu J
No affiliation info available
Wang W
No affiliation info available
Hu G
No affiliation info available
Song X
No affiliation info available
Wang Z
No affiliation info available

MeSH

AbsorptionAnimalsChromatography, High Pressure LiquidDrugs, Chinese HerbalGentianaIleumIridoid GlucosidesJejunumMalePyronesRatsRats, Wistar

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

22792800

Citation

Song, Bei, et al. "[Quickly Investigating the Absorption Ingredients of Gentianae Radix Et Rhizoma By SEMAC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 37, no. 7, 2012, pp. 969-73.
Song B, Zhu J, Wang W, et al. [Quickly investigating the absorption ingredients of Gentianae Radix et Rhizoma by SEMAC]. Zhongguo Zhong Yao Za Zhi. 2012;37(7):969-73.
Song, B., Zhu, J., Wang, W., Hu, G., Song, X., & Wang, Z. (2012). [Quickly investigating the absorption ingredients of Gentianae Radix et Rhizoma by SEMAC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 37(7), 969-73.
Song B, et al. [Quickly Investigating the Absorption Ingredients of Gentianae Radix Et Rhizoma By SEMAC]. Zhongguo Zhong Yao Za Zhi. 2012;37(7):969-73. PubMed PMID: 22792800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Quickly investigating the absorption ingredients of Gentianae Radix et Rhizoma by SEMAC]. AU - Song,Bei, AU - Zhu,Jingjing, AU - Wang,Weihao, AU - Hu,Guang, AU - Song,Xiaomei, AU - Wang,Zhimin, PY - 2012/7/17/entrez PY - 2012/7/17/pubmed PY - 2012/8/29/medline SP - 969 EP - 73 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 37 IS - 7 N2 - OBJECTIVE: To establish a method for quickly investigating the absorption ingredients which could be used as the index of quality control of Gentianae Radix et Rhizoma. METHOD: The absorption ingredients of Gentianae Radix et Rhizoma were investigated by using the model of in vitro everted intestinal sac (VEIS). The intestinal sac liquors of jejunum and ileum were collected at 6 intervals (15, 30, 45, 60, 90, 120 min) and gentiopicroside, loganin acid, swertiamarin and sweroside were detected by HPLC as the representative marker. The accumulative absorption quantity of gentiopicroside, loganin acid, swertiamarin and sweroside were calculated, respectively. RESULT: Six components could be detected in intestinal sac. In different concentrations of Gentianae Radix et Rhizoma, gentiopicroside and swertiamarin in various intestinal sections were the linear absorption (R2 > 0.9), conformed to zero order absorption rate. In jejunum the constant of absorption rate (Ka) of gentiopicroside and swertiamarin increased with the raised dosage of Gentianae Radix et Rhizoma (P < 0.05), which indicated a passive absorption manner, and the value of Ka of high and middle dosage of those in ileum were higher than that of low dosage, and the difference of Ka between high and middle dosage were not significant, which indicated a positive absorption manner. The Ka of high and middle dosage of sweroside in ileum and jejunum were higher than that of low dosage (P < 0.05), and the difference of Ka between high and middle dosage were not significant, which indicated a positive absorption manner. The Ka of loganin acid in jejunum and ileum increased along with the raised dosage of Gentianae Radix et Rhizoma (P < 0.05), which indicated a passive absorption manner. CONCLUSION: SEMAC could be used as a tool to investigate the absorption ingredients of Gentianae Radix et Rhizoma. Drug in intestine sac was selective, and the absorption part of intestine was also different. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/22792800/[Quickly_investigating_the_absorption_ingredients_of_Gentianae_Radix_et_Rhizoma_by_SEMAC]_ L2 - https://antibodies.cancer.gov/detail/CPTC-RRM2-2 DB - PRIME DP - Unbound Medicine ER -